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Molecular cloning and expression of cDNA for human granulocyte colony-stimulating factor

Naturevolume 319, pages415–418 (1986)Cite this article

Abstract

Granulocyte colony-stimulating factor (G-CSF) is a member of the CSF family of hormone-like glycoproteins that regulate haematopoietic cell proliferation and differentiation1,2, and G-CSF almost exclusively stimulates the colony formation of granulocytes from committed precursor cells in semi-solid agar culture2. Recently, Nomuraet al.3 have established a human squamous carcinoma cell line (designated CHU-2) from a human oral cavity tumour which produces large quantities of CSF constitutively, and the CSF produced by CHU-2 cells has been purified to homogeneity from the conditioned medium. We have now determined the partial amino-acid sequence of the purified G-CSF protein, and by using oligonucleotides as probes, have isolated several clones containing G-CSF complementary DNA from the cDNA library prepared with messenger RNA from CHU-2 cells. The complete nucleotide sequences of two of these cDNAs were determined and the expression of the cDNA in monkey COS cells gave rise to a protein showing authentic G-CSF activity. Furthermore, Southern hybridization analysis of DNA from normal leukocytes and CHU-2 cells suggests that the human genome contains only one gene for G-CSF and that some rearrangement has occurred within one of the alleles of the G-CSF gene in CHU-2 cells.

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Author information

Author notes

  1. Tatsumi Yamazaki, Osami Yamamoto, Yuichi Hirata, Naoki Kubota, Masayoshi Oheda, Hitoshi Nomura and Masayoshi Ono: New Drug Research Laboratories, Chugai Pharmaceutical Co., 3-41-8 Takada, Toshima-ku, Tokyo 171, Japan

Authors and Affiliations

  1. Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo, 108, Japan

    Shigekazu Nagata, Masayuki Tsuchiya, Shigetaka Asano, Yoshito Kaziro, Tatsumi Yamazaki, Osami Yamamoto, Yuichi Hirata, Naoki Kubota, Masayoshi Oheda, Hitoshi Nomura & Masayoshi Ono

Authors
  1. Shigekazu Nagata
  2. Masayuki Tsuchiya
  3. Shigetaka Asano
  4. Yoshito Kaziro
  5. Tatsumi Yamazaki
  6. Osami Yamamoto
  7. Yuichi Hirata
  8. Naoki Kubota
  9. Masayoshi Oheda
  10. Hitoshi Nomura
  11. Masayoshi Ono

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Nagata, S., Tsuchiya, M., Asano, S.et al. Molecular cloning and expression of cDNA for human granulocyte colony-stimulating factor.Nature319, 415–418 (1986). https://doi.org/10.1038/319415a0

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