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Point mutations close to the AUG initiator codon affect the efficiency of translation of rat preproinsulinin vivo
Naturevolume 308, pages241–246 (1984)Cite this article
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Abstract
To determine whether sequence context influences the ability of an AUG triplet to be recognized as an initiator codon by eukaryotic ribosomes, single nucleotide changes were introduced near the translational start site in a cloned preproinsulin gene. Maximum synthesis of preproinsulin occurred when a purine, preferably adenosine, was located three nucleotides upstream from the initiator codon. Adenosine is found most frequently in that position in natural mRNAs.
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Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, 15260, USA
Marilyn Kozak
- Marilyn Kozak
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Kozak, M. Point mutations close to the AUG initiator codon affect the efficiency of translation of rat preproinsulinin vivo.Nature308, 241–246 (1984). https://doi.org/10.1038/308241a0
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