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Abstract
Variation in mitochondrial DNA (mtDNA) was used together with comparative cytogenetics to examine the evolutionary history and taxonomic status of an African hystricomorphous rodent, the springharePedetes capensis. The mtDNA phylogeographic structure showed that the majority of the southern African populations (P. c. capensis) are characterized by unique but closely related maternal lineages. Based on restriction endonuclease fragment analysis, the east African populations (P. c. surdaster) appear more structured and are distinguished from those in southern Africa by an average sequence divergence of 5.52% (±1.4%). This marked divergence is concordant with results of the cytogenetic study. Specimens from southern Africa have 2n = 38, and those from east Africa 2n = 40. The change in diploid number is due to a single centric fusion. It is suggested that the closure of theBrachystegia or “miombo” woodland (20,000–10,000 B.P.), which delimits contemporary springhare ranges, may have been too recent to account for the accumulated genetic differences that distinguish these taxa. While rifting and associated habitat changes in east Africa can be invoked to explain genetic structure in this region, the southern African springhare populations, which have a high incidence of locality-specific haplotypes, show a shallow phylogeographic structure, in keeping with a relatively recent range expansion from smaller source populations. Given the magnitude of genetic, morphological, and ethological differences between the two geographic isolates, we believe that there is strong support for the elevation of the east African and southern African springhare populations to full species status, thus supporting earlier taxonomic treatments of this rodent.
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REFERENCES
Amos, B., and Hoelzel, A. R. (1991). Long-term preservation of whale skin for DNA analysis.Rep. Int. Whaling Comm. (Spec. Issue)13: 99–104.
Avise, J. C. (1989). Gene trees and organismal histories: A phylogenetic approach to population biology.Evolution43: 1192–1208.
Avise, J. C. (1994).Molecular Markers, Natural History and Evolution, Chapman and Hall, London and New York.
Avise, J. C., and Ball, R. M. (1990). Principles of genealogical concordance in species concepts and biological taxonomy.Oxford Surv. Evol. Biol.7: 45–67.
Avise, J. C., Giblin-Davidson, C., Laerm, J., Patton, J. C., and Lansman, R. A. (1979a). Mitochondrial DNA clones and matriarchal phylogeny within and among geographic populations of the pocket gopher,Geomys pinetis.Proc. Natl. Acad. Sci. U.S.A.76: 6694–6698.
Avise, J. C., Lansman, R. A., and Shade, R. O. (1979b). The use of restriction endonucleases to measure mitochondrial DNA sequence relatedness in natural populations. I. Population structure and evolution in the genusPeromyscus.Genetics92: 279–295.
Avise, J. C., Bowen, B. W., and Lamb, T. (1989). DNA fingerprints from hypervariable mitochondrial genotypes.Mol. Biol. Evol.6: 258–269.
Ball, R. M., Freeman, S., James, F. C., Bermingham, E., and Avise, J. C. (1988). Phylogeographic population structure of red-winged blackbirds assessed by mitochondrial DNA.Proc. Natl. Acad. Sci. U.S.A.85: 1558–1562.
Brown, L. H., Urban, E. K., and Newman, K. (1982).The Birds of Africa, Vol. 1, Academic Press, London.
Brown, W. M. (1980). Polymorphism in mitochondrial DNA of humans.Proc. Natl. Acad. Sci. U.S.A.77: 3605–3609.
Brown, W. M. (1983). Evolution of animal mitochondrial DNA. In:Evolution of Genes and Proteins, M. Nei and R. K. Koehn, eds., pp. 63–88, Sinauer, Sunderland.
Bush, G. L., Case, S. M., Wilson, A. C., and Patton, J. L. (1977). Rapid speciation and chromosomal evolution in mammals.Proc. Natl. Acad. Sci. U.S.A.74: 3942–3946.
Chesser, R. K. (1983). Isolation by distance: relationship to the management of genetic resources. In:Genetics and Conservation, C. M. Schonewald-Cox, S. M. Chambers, B. MacBryde, and L. Thomas, eds., pp. 66–77, Benjamin/Cummings, London.
Coe, M. J., and Skinner, J. D. (1993). Connections, disjunctions and endemism in the eastern and southern African mammal faunas.Trans. R. Soc. S. Afr.48: 233–255.
Crandall, K. A., and Templeton, A. R. (1993). Empirical tests of some predictions from coalescent theroy with applications to intraspecific phylogeny reconstruction.Genetics134: 959–969.
Crandall, K. A., Templeton, A. R., and Sing, C. F. (1994). Intraspecific phylogenetics: problems and solutions. In:Models in Phylogeny Reconstruction, R. W. Scotland, D. J. Siebert, and D. M. Williams, eds., pp. 273–279, Claredon Press, Oxford.
Cronin, M. A., Amstrup, S. C., and Garner, G. W. (1991). Interspecific and intraspecific mitochondrial DNA variation in North American bears(Ursus).Can. J. Zool.69: 2985–2992.
Da Silva, M. N. F., and Patton, J. L. (1993). Amazonian phylogeography: mtDNA sequence variation in arboreal echimyid rodents (Caviomorpha).Mol. Phyl. Evol.2: 243–255.
Davies, C. (1982).The Recent and Fossil Affinities of the Genus Pedetes (Mammalia: Rodentia), Doctor of Philosophy thesis, St. Peter's College, Oxford.
Deacon, H. J., and Thackeray, J. F. (1983). Late Pleistocene environmental changes and implications for archaeological records in southern Africa. In:Proceedings of SASQUA International Symposium, J. C. Vogel, ed., pp. 375–390, AA Balkema, Rotterdam.
De Graaff, G. (1981). Sciuromorpha. In:The Rodents of Southern Africa, pp. 39–43, Butterworths Press, Durban.
Dieterlen, F. (1993). Family Pedetidae. In:Mammal Species of the World: A Taxonomic and Geographic Reference, 2nd ed., D. E. Wilson and D. M. Reeder, eds., p. 759, Smithsonian Institution Press, Washington, DC.
Dowling, T. E., Moritz, C., and Palmer, J. D. (1990). Nucleic acids II: Restriction site analysis. In:Molecular Systematics, D. M. Hillis and C. Moritz, eds., pp. 250–317, Sinauer, Sunderland, MA.
Eisenberg, J. F. (1981).The Mammalian Radiations: An Analysis of Trends in Evolution, Adaptation, and Behaviour, Athlone Press, London.
Ellerman, J. R., Morrison-Scott, T. C. S., and Hayman, R. W. (1953). Family Pedetidae. In:Southern African Mammals 1758 to 1951: A Reclassification, pp. 251–252, British Museum, London.
Feinberg, A. P., and Vogelstein, B. (1983). A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.Anal. Biochem.132: 6–13.
Freitag, S., and Robinson, T. J. (1993). Phylogeographic patterns in the mitochondrial DNA of the ostrich (Struthio camelus).Auk110: 614–622.
Hamilton, A. C. (1976). The significance of patterns of distribution shown by forest plants and animals in tropical Africa for the reconstruction of palaeoenvironments: A review.Palaeoecol. Africa9: 63–97.
Hamilton, A. C. (1982).Environmental History of East Africa. A Study of the Quaternary, Academic Press, London.
Hayes, J. P., and Harrison, R. G. (1992). Variation in mitochondrial DNA and the biogeographic history of woodrats (Neotoma) of the eastern United States.Syst. Biol.41: 331–344.
Hillis, D. M., and Huelsenbeck, J. P. (1992). Signal, noise, and reliability in molecular phylogenetic analysis.J. Hered.13: 189–195.
Hillis, D. M., and Moritz, C. (eds.) (1990).Molecular Systematics, Sinauer Associates, Sunderland.
Hollister, N. (1919). East African mammals in the United States National Museum. II Rodentia, Lagomorpha and Tubulidentata.Bull. U.S. Nat. Mus99: 1–184.
Honeycutt, R. L., Edwards, S. V., Nelson, K., and Nevo, E. (1987). Mitochondrial DNA variation and the phylogeny of African mole rats (Rodentia: Bathyergidae).Syst. Zool.36: 280–292.
Hsu, T. C., and Benirschke, K. (1977).An Atlas of Mammalian Chromosomes, Vol. 10, Folio 456, Springer-Verlag, New York.
Jaarola, M., and Tegelström, H. (1995). Colonization history of north European field voles (Microtus agrestis) revealed by mitochondrial DNA.Mol. Ecol.4: 299–310.
King, M. (1993).Species Evolution: The Role of Chromosome Change, Cambridge University Press, Cambridge.
Kingdon, J. (1971).East African Mammals: An Atlas of Evolution in Africa, Vol. I, Academic Press, London.
Kingdon, J. (1990).Island Africa: The Evolution of Africa's Rare Animals and Plants, Collins, London.
Lansman, R. A., Shade, R. O., Shapira, J. F., and Avise, J. C. (1981). The use of restriction endonucleases to measure mitochondrial DNA sequence relatedness in natural populations: III techniques and potential applications.J. Mol. Evol.17: 214–226.
Lansman, R., A. Avise, J. C., Aquadro, C. F., Shapira, J. F., and Daniel, S. W. (1983). Extensive genetic variation in mitochondrial DNA's among geographic populations of the deer mouse,Peromyscus maniculatus.Evolution37: 1–16.
Luckett, W. P., and Hartenberger, J.-L. (eds.) (1985).Evolutionary Relationships Among Rodents: A Multidisciplinary Analysis. Plenum Press, New York.
MacNeil, D., and Strobeck, C. (1987). Evolutionary relationships among colonies of Columbian ground squirrels as shown by mitochondrial DNA.Evolution41: 873–881.
Matthee, C. A., and Robinson, T. J. (1996). Mitochondrial DNA differentiation among geographical populations ofPronolagus rupestris, Smith's red rock rabbit (Mammalis: Lagomorpha).Heredity76: 514–523.
Matthee, C. A., and Robinson, T. J. (1997). Molecular phylogeny of the springhare,Pedetes capensis, based on mitochondrial DNA sequences.Mol. Biol. Evol.14: 20–29.
McKnight, M. L. (1995). Mitochondrial DNA phylogeography ofPerognathus amplus andPerognathus longimembris (Rodentia: Heteromyidae): A possible mammalian ring species.Evolution49: 816–826.
McMillan, W. O., and Bermingham, E. (1996). The phylogeographic pattern of mitochondrial DNA variation in the Dall's porpoisePhocoenoides dalli.Mol. Ecol.5: 47–61.
Meester, J. A. J., Rautenbach, I. L., Dippenaar, N. J., and Baker, C. M. (1986).Classification of Southern African Mammals, Trans. Mus. Monogr. 5, Pretoria.
Misonne, X. (1974). Rodentia. In:The Mammals of Africa: An Identification Manual, J. Meester and H. W. Setzer, eds., p. 8, Smithsonian Institution Press, Washington, DC.
Nei, M. (1987). Evolutionary changes of nucleotide sequences. In:Molecular Evolutionary Genetics, pp. 64–110, Columbia University Press, New York.
Nei, M., and Miller, J. C. (1990). A simple method for estimating average number of nucleotide substitutions within and between populations from restriction fragment data.Genetics125: 873–879.
Nei, M., and Tajima, F. (1981). DNA polymorphism detectable by restriction endonucleases.Genetics97: 145–163.
Phillips, C. A. (1994). Geographic distribution of mitochondrial DNA variants and historical biogeography of the spotted salamander.Ambystoma maculatum.Evolution48: 597–607.
Plante, Y., Boag, P.T., and White, B. N. (1989). Macrogeographic variation in mitochondrial DNA of meadow voles (Microtus pennsylvanicus).Can. J. Zool.67: 158–166.
Quennell, A. M. (1982).Rift Valleys, Afro-Arabian, Hutchinson Ross, Philadelphia.
Riddle, B. R., and Honeycutt, R. L. (1990). Historical biogeography in north American arid regions: An approach using mitochondrial DNA phylogeny in grasshopper mice (genusOnychomys).Evolution44: 1–15.
Roberts, A. (1951). Pedetidae. In:The Mammals of South Africa, R. Bigalke, V. Fitzsimons, and D. E. Malan, eds., pp. 350–352, Trustees of ‘The Mammals of South Africa’ book fund, Johannesburg.
Saitou, N., and Nei, M. (1987). The neighbor-joining method: A new method for reconstructing phylogenetic trees.Mol. Biol. Evol.4: 406–425.
Slatkin, M. (1987). Gene flow and the geographic structure of natural populations.Science236: 787–792.
Smithers, R. H. N. (1968). Family Pedetidae. In:A Check List and Atlas of the Mammals of Botswana, p. 30, Variprint, Salisbury.
Smithers, R. H. N. (1983).The Mammals of the Southern African Subregion, University of Pretoria, Pretoria.
Southern, E. M. (1975). Detection of specific sequences among DNA fragments separated by gel electrophoresis.J. Mol. Biol.98: 503–517.
Sumner, A. T. (1972). A simple technique for demonstrating centromeric heterochromatin.Exp. Cell Res.75: 304–306.
Swofford, D. L. (1993).PAUP: Phylogenetic Analysis Using Parsimony, Version 3.1.2d5, Illinois Natural History Survey, Champaign.
Thackeray, J. F. (1987). Late Quaternary environmental changes inferred from small mammalian fauna, southern Africa.Climatic Change10: 285–305.
Thomas, O. (1902).Pedetes. Ann. Mag. Nat. Hist.7: 440.
Verdcourt, B. (1969). The arid corridor between north-east and south-west areas of Africa.Palaeoecol. Africa4: 140–144.
Walker, E. P. (1975).Mammals of the World, 3rd. ed., J. L. Paradiso ed., p. 755, John Hopkins University Press, Baltimore.
Walton, C. (1984).Reader's Digest Atlas of Southern Africa, J. Bartholomew & Son, Edinburgh.
Wilson, A. C., Bush, G. L. Case, S. M., and King, M. (1975). Social structuring of mammalian populations and rate of chromosomal evolution.Proc. Natl. Acad. Sci. U.S.A.72: 5061–5065.
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Department of Zoology and Entomology, University of Pretoria, Pretoria, 0002, South Africa
C. A. Matthee & T. J. Robinson
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Matthee, C.A., Robinson, T.J. Mitochondrial DNA Phylogeography and Comparative Cytogenetics of the Springhare,Pedetes capensis (Mammalia: Rodentia).Journal of Mammalian Evolution4, 53–73 (1997). https://doi.org/10.1023/A:1027331727034
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