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Upregulation of mRNA Encoding the M5 Muscarinic Acetylcholine Receptor in Human T- and B-Lymphocytes During Immunological Responses

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Abstract

Lymphocytes possess an independent, non-neuronal cholinergic system. Moreover, both T- and B-lymphocytes express multiple muscarinic acetylcholine receptors (mAChR). To obtain a better understanding of the regulatory mechanisms governing mAChR gene expression in the lymphocytic cholinergic system, we examined the effects of lymphocyte activation on expression of mAChR mRNA. Stimulation of T- and B-lymphocytes, respectively, with T-cell activator phytohemagglutinin and B-cell activatorStaphylococcus aureus Cowan I upregulated M5 mAChR mRNA expression in the CEM human leukemic T-cell line and in the Daudi B-cell line, which served as models of lymphocytes. In striking contrast, M3 and M4 mAChR mRNA expression was not affected in either cell line. Nonetheless, stimulating lymphocytes with phorbol 12-myristate 13-acetate, a protein kinase C activator, plus ionomycin, a calcium ionophore, upregulated expression of both M3 and M5 mAChR mRNA. This represents the first demonstration that immunological stimulation leads to M5 mAChR gene expression in lymphocytes.

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Authors and Affiliations

  1. Department of Pharmacology, Kyoritsu College of Pharmacy, 1–5–30 Shibakoen, Minato-ku, Tokyo, 105–8512, Japan

    Takeshi Fujii, Yoshihiro Watanabe, Tomoyuki Inoue & Koichiro Kawashima

Authors
  1. Takeshi Fujii

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  2. Yoshihiro Watanabe

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  3. Tomoyuki Inoue

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  4. Koichiro Kawashima

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Correspondence toKoichiro Kawashima.

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