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Abstract
GenusSilene L. (Caryophyllaceae) contains about 700 species divided into 44 sections. According to recent taxonomic classification this genus also includes taxa previously classified in generaLychnis andMelandrium. In this work, fourSilene species belonging to different sections were studied:S. latifolia (syn.Melandrium album, Section Elisanthe),S. vulgaris (Inflatae),S. pendula (Erectorefractae), andS. chalcedonica (syn.Lychnis chalcedonica, Lychnidiformes). Flow cytometric analysis revealed a genome size of 2.25 and 2.35 pg/2C forS. vulgaris andS. pendula and of 5.73 and 6.59 pg/2C forS. latifolia andS. chalcedonica. All four species have the same chromosome number including the pair of sex chromosomes of the dioeciousS. latifolia (2n=2x=24). Double target fluorescencein-situ hybridization revealed the chromosomal locations of 25S rDNA and 5S rDNA. A marked variation in number and localization of rDNA loci but no correlation between the numbers of rDNA clusters and genome size was found. FISH and genome size data indicate that nuclear genomes ofSilene species are highly diversified as a result of numerous DNA amplifications and translocations.
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Institute of Biophysics, Academy of Sciences of the Czech Republic, CZ-612 65, Brno, Czech Republic
Jiří Široký, Eduard Kejnovský & Boris Vyskot
Institute of Experimental Botany, Academy of Sciences of the Czech Republic, CZ-772 00, Olomouc, Czech Republic
Martin A. Lysák & Jaroslav Doležel
- Jiří Široký
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- Martin A. Lysák
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Široký, J., Lysák, M.A., Doležel, J.et al. Heterogeneity of rDNA distribution and genome size inSilene spp..Chromosome Res9, 387–393 (2001). https://doi.org/10.1023/A:1016783501674
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