Movatterモバイル変換


[0]ホーム

URL:


Skip to main content

Advertisement

Springer Nature Link
Log in

Heterogeneity of rDNA distribution and genome size inSilene spp.

  • Published:
Chromosome Research Aims and scope Submit manuscript

Abstract

GenusSilene L. (Caryophyllaceae) contains about 700 species divided into 44 sections. According to recent taxonomic classification this genus also includes taxa previously classified in generaLychnis andMelandrium. In this work, fourSilene species belonging to different sections were studied:S. latifolia (syn.Melandrium album, Section Elisanthe),S. vulgaris (Inflatae),S. pendula (Erectorefractae), andS. chalcedonica (syn.Lychnis chalcedonica, Lychnidiformes). Flow cytometric analysis revealed a genome size of 2.25 and 2.35 pg/2C forS. vulgaris andS. pendula and of 5.73 and 6.59 pg/2C forS. latifolia andS. chalcedonica. All four species have the same chromosome number including the pair of sex chromosomes of the dioeciousS. latifolia (2n=2x=24). Double target fluorescencein-situ hybridization revealed the chromosomal locations of 25S rDNA and 5S rDNA. A marked variation in number and localization of rDNA loci but no correlation between the numbers of rDNA clusters and genome size was found. FISH and genome size data indicate that nuclear genomes ofSilene species are highly diversified as a result of numerous DNA amplifications and translocations.

This is a preview of subscription content,log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+
from ¥17,985 /Month
  • Starting from 10 chapters or articles per month
  • Access and download chapters and articles from more than 300k books and 2,500 journals
  • Cancel anytime
View plans

Buy Now

Price includes VAT (Japan)

Instant access to the full article PDF.

Similar content being viewed by others

Explore related subjects

Discover the latest articles, books and news in related subjects, suggested using machine learning.

References

  • Bennett MD, Cox AV, Leitch IJ (1998) Angiosperm DNA C-values database. http://www.rbgkew.org.uk/eval/databasel.html

  • Castilho A, Heslop-Harrison JS (1995) Physical mapping of 5S and 18S–25S rDNA and repetitive DNA sequences inAegilopsumbellulata.Genome38: 91-96

    Google Scholar 

  • Charlesworth D, Guttman DS (1999) The evolution of dioecy and plant sex chromosome systems. In: Ainsworth CC, ed.Sex Determination in Plants. Oxford: Bios Scientific Publishers, pp 25-49.

    Google Scholar 

  • Chowdhuri PK (1957) Studies in the genusSilene.Notes Roy Bot Gard Edinburgh22: 221-278.

    Google Scholar 

  • Ciupercescu DD, Veuskens J, Mouras A, Ye D, Briquet M, Negrutiu I (1990) KaryotypingMelandrium album, a dioecious plant with heteromorphic sex chromosomes.Genome33: 556-562.

    Google Scholar 

  • Degraeve N (1980) Étude de diverses particularités caryotypiques des genresSilene, Lychnis etMelandrium.Boletim Sociedade Broteriana53: 595-643.

    Google Scholar 

  • Desfeux C, Lejeune B (1996) Systematics of EuromediterraneanSilene (Caryophyllaceae): evidence from a phylogenetic analysis using ITS sequences.CR Acad Sci Paris319: 351-358.

    Google Scholar 

  • Desfeux C, Maurice S, Henry J-P, Lejeune B, Gouyon P-II (1996) Evolution of reproductive systems in the genusSilene.Proc Roy Soc London B263: 409-414.

    Google Scholar 

  • Doležel J, Sgorbati S, Lucretti S (1992) Comparison of three DNA fluorochromes for flow cytometric estimation of nuclear DNA content in plants.Physiol Plant85: 625-631.

    Google Scholar 

  • Erdmann VA, Wolters J (1986) Collection of published 5S, 5.8S and 4.5S ribosomal RNA sequences.Nucleic Acids Res15: R1-R59.

    Google Scholar 

  • Fulneček J, Matyášek R, Kovařík A, Bezděk M (1998) Mapping of 5-methylcytosine residues inNicotiana tabacum 5S rRNA genes by genomic sequencing.Mol Gen Genet259: 133-141.

    Google Scholar 

  • Greuter W, Burdet HM, Long G (1984) Med-Checklist. A critical inventory of vascular plants of the circum-mediterranean countries, 1,Pteridophyta, Gymnospermae, Dicotyledones (Acanthaceae — Cneoraceae). Conservatoire et Jardin Botaniques. Genéve: Botanischer Garten und Botanisches Museum Berlin.

    Google Scholar 

  • Hladilová R, Široký J, Vyskot B (1998) A cytospin technique for spreading plant metaphases suitable for immunofluorescence studies.Biotechnic Histochem73: 150-156.

    Google Scholar 

  • Howell WM, Black DA (1980) Controlled silver-staining of nucleous organizer regions with a protective colloidal developer: a 1-step method.Experientia36: 1014-1015.

    Google Scholar 

  • Kiss T, Kis M, Solomosy F (1989) Nucleotide sequence of a 25S rRNA gene from tomato.Nucleic Acids Res17: 7179.

    Google Scholar 

  • Leitch AW (2000) Higher levels of organization in the interphase nucleus of cycling and differentiated cells.Microbiol Mol Biol Rev64: 138-152.

    Google Scholar 

  • Lysák MA, Doležel J (1998) Estimation of nuclear DNA content inSesleria (Poaceae).Caryologia52: 123-132.

    Google Scholar 

  • Oxelmann B, Lindén M (1995) Generic boundaries in the tribeSileneae (Caryophyllaceae) as inferred from nuclear rDNA sequences.Taxon44: 525-542.

    Google Scholar 

  • Pontes O, Cotrim H, Pais S, Viegas W (2000) Physical mapping, expression patterns and interphase organization of rDNAloci in Portuguese endemicSilene cintrana andSilene rothmaleri.Chromosome Res8: 313-317.

    Google Scholar 

  • Roose ML, Schwarzacher T, Heslop-Harrison JS (1998) The chromosomes ofCitrus andPorcinus species and hybrids: Identification of characteristic chromosomes and physical mapping of rDNA loci usingin situ hybridization and fluorochrome banding.J Hered89: 83-86.

    Google Scholar 

  • Sone T, Fujisawa M, Takenaka Met al. (1999) Bryophyte 5S rDNA was inserted into 45S rDNA repeat units after the divergence from higher land plants.Plant Mol Biol41: 679-685.

    Google Scholar 

  • Taketa S, Harrison GE, Heslop-Harrison JS (1999) Comparative physical mapping of the 5S and 18–25S rDNA in nine wildHordeum species and cytotypes.Theor Appl Genet98: 1-9.

    Google Scholar 

  • Vagera J, Paulíková D, Doležel J (1994) The development of male and female regenerants byin vitro androgenesis in dioccious plantMelandrium album.Ann Bot73: 455-459.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Biophysics, Academy of Sciences of the Czech Republic, CZ-612 65, Brno, Czech Republic

    Jiří Široký, Eduard Kejnovský & Boris Vyskot

  2. Institute of Experimental Botany, Academy of Sciences of the Czech Republic, CZ-772 00, Olomouc, Czech Republic

    Martin A. Lysák & Jaroslav Doležel

Authors
  1. Jiří Široký
  2. Martin A. Lysák
  3. Jaroslav Doležel
  4. Eduard Kejnovský
  5. Boris Vyskot

Rights and permissions

About this article

Access this article

Subscribe and save

Springer+
from ¥17,985 /Month
  • Starting from 10 chapters or articles per month
  • Access and download chapters and articles from more than 300k books and 2,500 journals
  • Cancel anytime
View plans

Buy Now

Price includes VAT (Japan)

Instant access to the full article PDF.

Advertisement


[8]ページ先頭

©2009-2026 Movatter.jp