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Introgression and genetic mapping of leaf rust and stripe rust resistance inAegilops triuncialis

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Abstract

The growing and cultivating resistant wheat crop varieties is important to meet the demands of the growing population and minimizing the yield losses due to foliar diseases. More important is the identification of novel resistance sources and transfer of resistance in ready to use form. In the current study, leaf rust (LR) and stripe rust (YR) resistant tetraploid nonprogenitors of wheatAegilops triuncialis (UtUtCtCt) acc pau 3462 was crossed and backcrossed susceptible cultivar WL711(NN) by inducing homeologous pairing using CSph1. Recurrent parent type plants were selected in subsequent generation with resistance to LR and YR and BC2F7 introgression line (2n=42) namedILtri have been developed. To understand the nature and inheritance of LR and YR resistance genes and to map their genomic location, F2 and F2:3 mapping populations were developed by crossingILtri with WL711(NN). In F2 and F2:3, the seedlings and adult plants segregated into 3R:1S and 1HR:2Seg:1HS ratios, respectively for both LR and YR, indicating inheritance of single dominant all stage resistance gene working against both the rusts. These genes were temporary designated asLrtri andYrtri and were inherited independently. Molecular mapping of 614 SSR markers mapped theLrtri at a distance of 11.2 cM from SSR markerXwmc606.

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Acknowledgements

The supply of the rust inoculum by Indian Institute of Wheat and Barley Research Station, Flowerdale, Shimla, India is gratefully acknowledged.

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Author notes
  1. SA, conducted experiment, did all phenotyping, molecular mapping, manuscript writing. SK and PC, conceived and designed research, developed introgression lines. RS, helped in molecular mapping. GSD, SK and SA produced final draft of manuscript. AS helped material development and final selection of introgression lines. JK, screening against yellow rust, maintenance of different races of brown rust and yellow rust.

Authors and Affiliations

  1. School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, 141 004, India

    Shiksha Arora, Satinder Kaur, Guriqbal Singh Dhillon, Rohtas Singh & Parveen Chhuneja

  2. Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana, 141 004, India

    Jaspal Kaur & Achla Sharma

Authors
  1. Shiksha Arora
  2. Satinder Kaur
  3. Guriqbal Singh Dhillon
  4. Rohtas Singh
  5. Jaspal Kaur
  6. Achla Sharma
  7. Parveen Chhuneja

Corresponding author

Correspondence toSatinder Kaur.

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Corresponding editor:H. A. Ranganath

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Arora, S., Kaur, S., Dhillon, G.S.et al. Introgression and genetic mapping of leaf rust and stripe rust resistance inAegilops triuncialis.J Genet100, 6 (2021). https://doi.org/10.1007/s12041-020-01253-3

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