- Shanshan Jiang1,
- Gulcin Yildiz2,
- Junzhou Ding1,
- Juan Andrade1,
- Taha M. Rababahb3,
- Ali Almajwalc4,
- Mahmoud M. Abulmeatyc4 &
- …
- Hao Feng1 nAff5
2485Accesses
81Citations
Abstract
Pea protein is one of the few hypoallergenic vegetable proteins without genetic modification issues. Pea protein isolate (PPI) is a natural emulsifier with high nutritional value. Nevertheless, PPI’s applications in foods are limited by its relatively poor solubility and functional properties. This study was performed to explore the use of a pH-shifting and sonication combined treatment to modify the functional properties of PPI and use the soluble pea protein nanoaggregates produced by the method as base materials to form nanoemulsion and nanocomplex. The ability of these nanocarriers to protect cholecalciferol (vitamin D3) against UV radiation was examined. The stability of nanostructures during storage, antioxidant capacity, and in vitro digestion was evaluated. Generally, the modified PPI-prepared nanoemulsions over-performed the nanocomplexes in all experiments and both showed good protection of vitamin D3 against UV radiation. The stability of the pea protein nanostructures was confirmed in 30-day storage. An improved antioxidant capacity was observed in the ultrasound-treated pea proteins. The modified PPI-prepared nanoemulsions exhibited a significantly higher recovery of vitamin D3 in micelles through in vitro digestion. Therefore, the modified PPI-prepared nanoemulsion may be used as a good natural carrier for protection and delivery of health-promoting compounds in foods.
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References
Abbasi, A., Emam-Djomeh, Z., Mousavi, M. A. E., & Davoodi, D. (2014). Stability of vitamin D3 encapsulated in nanoparticles of whey protein isolate.Food Chemistry, 143, 379–383.https://doi.org/10.1016/j.foodchem.2013.08.018.
Almouazen, E., Bourgeois, S., Jordheim, L. P., Fessi, H., & Briancon, S. (2013). Nano-encapsulation of vitamin D3 active metabolites for application in chemotherapy: formulation study and in vitro evaluation.Pharmaceutical Research, 30(4), 1137–1146.https://doi.org/10.1007/s11095-012-0949-4.
Apostolakis, M., Armeni, E., Bakas, P., & Lambrinoudaki, I. (2018). Vitamin D and cardiovascular disease.Maturitas, 115(May), 1–22.https://doi.org/10.1016/j.maturitas.2018.05.010.
Diarrassouba, F., Garrait, G., Remondetto, G., Alvarez, P., Beyssac, E., & Subirade, M. (2015). Improved bioavailability of vitamin D3 using a β-lactoglobulin-based coagulum.Food Chemistry, 172, 361–367.https://doi.org/10.1016/j.foodchem.2014.09.054.
Donsi, F., Senatore, B., Huang, Q., & Ferrari, G. (2010). Development of novel pea protein-based nanoemulsions for delivery of nutraceuticals.Journal of Agricultural and Food Chemistry, 58(19), 10653–10660.https://doi.org/10.1021/jf101804g.
Donsì, F., Annunziata, M., Vincensi, M., & Ferrari, G. (2012). Design of nanoemulsion-based delivery systems of natural antimicrobials: effect of the emulsifier.Journal of Biotechnology, 159(4), 342–350.https://doi.org/10.1016/j.jbiotec.2011.07.001.
Estébanez, N., Gómez-Acebo, I., Palazuelos, C., Llorca, J., & Dierssen-Sotos, T. (2018). Vitamin D exposure and risk of breast cancer: a meta-analysis.Scientific Reports, 8(1), 1–13.https://doi.org/10.1038/s41598-018-27297-1.
Garewal, M., Zhang, L., & Ren, G. (2013). Optimized negative-staining protocol for examining lipid-protein interactions by electron microscopy. InMethods in molecular biology (Vol. 974, pp. 111–118). New York: Springer Science+Business Media.https://doi.org/10.1007/978-1-62703-275-9.
Garrett, D. A., Failla, M. L., & Sarama, R. J. (1999). Development of an in vitro digestion method to assess carotenoid bioavailability from meals.Journal of Agricultural and Food Chemistry, 47(10), 4301–4309.https://doi.org/10.1021/jf9903298.
Hedrén, E., Diaz, V., & Svanberg, U. (2002). Estimation of carotenoid accessibility from carrots determined by an in vitro digestion method.European Journal of Clinical Nutrition, 56(5), 425–430.https://doi.org/10.1038/sj.ejcn.1601329.
Hu, H., Wu, J., Li-Chan, E. C. Y., Zhu, L., Zhang, F., Xu, X., Fan, G., Wang, L., Huang, X., & Pan, S. (2013). Effects of ultrasound on structural and physical properties of soy protein isolate (SPI) dispersions.Food Hydrocolloids, 30(2), 647–655.https://doi.org/10.1016/j.foodhyd.2012.08.001.
Humiski, L. M., & Aluko, R. E. (2007). Physicochemical and bitterness properties of enzymatic pea protein hydrolysates.Journal of Food Science, 72(8), S605–S611.https://doi.org/10.1111/j.1750-3841.2007.00475.x.
Hur, S. J., Lim, B. O., Decker, E. A., & McClements, D. J. (2011). In vitro human digestion models for food applications.Food Chemistry, 125(1), 1–12.https://doi.org/10.1016/j.foodchem.2010.08.036.
Jiang, J., Xiong, Y. L., & Chen, J. (2011). Role of β-conglycinin and glycinin subunits in the pH-shifting-induced structural and physicochemical changes of soy protein isolate.Journal of Food Science, 76(2), 293–302.https://doi.org/10.1111/j.1750-3841.2010.02035.x.
Jiang, J., Zhu, B., Liu, Y., & Xiong, Y. L. (2014). Interfacial structural role of pH-shifting processed pea protein in the oxidative stability of oil/water emulsions.Journal of Agricultural and Food Chemistry, 62(7), 1683–1691.https://doi.org/10.1021/jf405190h.
Jiang, S., Ding, J., Andrade, J., Rababah, T. M., Almajwal, A., Abulmeaty, M. M., Feng, H. (2017). Modifying the physicochemical properties of pea protein by pH-shifting and ultrasound combined treatments,Ultrasonics Sonochemistry, 38, 835–842.
Kaltsa, O., Gatsi, I., Yanniotis, S., & Mandala, I. (2014). Influence of ultrasonication parameters on physical characteristics of olive oil model emulsions containing xanthan.Food and Bioprocess Technology, 7(7), 2038–2049.https://doi.org/10.1007/s11947-014-1266-1.
Lee, H., Yildiz, G., dos Santos, L. C., Jiang, S., Andrade, J. E., Engeseth, N. J., & Feng, H. (2016). Soy protein nano-aggregates with improved functional properties prepared by sequential pH treatment and ultrasonication.Food Hydrocolloids, 55, 200–209.https://doi.org/10.1016/j.foodhyd.2015.11.022.
Levinson, Y., Israeli-Lev, G., & Livney, Y. D. (2014). Soybean β-conglycinin nanoparticles for delivery of hydrophobic nutraceuticals.Food Biophysics, 9(4), 332–340.https://doi.org/10.1007/s11483-014-9341-8.
Li, Y., Jiang, B., Zhang, T., Mu, W., & Liu, J. (2008). Antioxidant and free radical-scavenging activities of chickpea protein hydrolysate (CPH).Food Chemistry, 106(2), 444–450.https://doi.org/10.1016/j.foodchem.2007.04.067.
Liang, H. N., & Tang, C. H. (2013). PH-dependent emulsifying properties of pea [Pisum sativum (L.)] proteins.Food Hydrocolloids, 33(2), 309–319.https://doi.org/10.1016/j.foodhyd.2013.04.005.
Luo, Y., Teng, Z., & Wang, Q. (2012). Development of zein nanoparticles coated with carboxymethyl chitosan for encapsulation and controlled release of vitamin D3.Journal of Agricultural and Food Chemistry, 60(3), 836–843.https://doi.org/10.1021/jf204194z.
McClements, D. J., & Rao, J. (2011). Food-grade nanoemulsions: formulation, fabrication, properties, performance, biological fate, and potential toxicity.Critical Reviews in Food Science and Nutrition, 51(4), 285–330.https://doi.org/10.1080/10408398.2011.559558.
Nik, A. M., Corredig, M., & Wright, A. J. (2011). Release of lipophilic molecules during in vitro digestion of soy protein-stabilized emulsions.Molecular Nutrition and Food Research, 55(S2), S278–S289.https://doi.org/10.1002/mnfr.201000572.
Overduin, J., Guérin-Deremaux, L., Wils, D., & Lambers, T. T. (2015). NUTRALYS pea protein: characterization of in vitro gastric digestion and in vivo gastrointestinal peptide responses relevant to satiety.Food and Nutrition Research, 59(1), 25622.https://doi.org/10.3402/fnr.v59.25622.
Pownall, T. L., Udenigwe, C. C., & Aluko, R. E. (2010). Amino acid composition and antioxidant properties of pea seed (Pisum sativum L.) enzymatic protein hydrolysate fractions.Journal of Agricultural and Food Chemistry, 58(8), 4712–4718.https://doi.org/10.1021/jf904456r.
Pownall, T. L., Udenigwe, C. C., & Aluko, R. E. (2011). Effects of cationic property on the in vitro antioxidant activities of pea protein hydrolysate fractions.Food Research International, 44(4), 1069–1074.https://doi.org/10.1016/j.foodres.2011.03.017.
Stefanović, A. B., Jovanović, J. R., Dojčinović, M. B., Lević, S. M., Nedović, V. A., Bugarski, B. M., & Knežević-Jugović, Z. D. (2017). Effect of the controlled high-intensity ultrasound on improving functionality and structural changes of egg white proteins.Food and Bioprocess Technology, 10(7), 1224–1239.https://doi.org/10.1007/s11947-017-1884-5.
Tang, B. M., Eslick, G. D., Nowson, C., Smith, C., & Bensoussan, A. (2007). Use of calcium or calcium in combination with vitamin D supplementation to prevent fractures and bone loss in people aged 50 years and older: a meta-analysis.Lancet, 370(9588), 657–666.https://doi.org/10.1016/S0140-6736(07)61342-7.
Teng, Z., Luo, Y., & Wang, Q. (2013). Carboxymethyl chitosan-soy protein complex nanoparticles for the encapsulation and controlled release of vitamin D3.Food Chemistry, 141(1), 524–532.https://doi.org/10.1016/j.foodchem.2013.03.043.
Yang, Y., & McClements, D. J. (2013). Vitamin E bioaccessibility: Influence of carrier oil type on digestion and release of emulsified α-tocopherol acetate.Food Chemistry, 141(1), 473–481.https://doi.org/10.1016/j.foodchem.2013.03.033.
Yildiz, G., Andrade, J., Engeseth, N. E., & Feng, H. (2017). Functionalizing soy protein nano-aggregates with pH-shifting and mano-thermo-sonication.Journal of Colloid and Interface Science, 505, 836–846.https://doi.org/10.1016/j.jcis.2017.06.088.
Yildiz, G., Ding, J., Andrade, J., Engeseth, N. J., & Feng, H. (2018a). Effect of plant protein-polysaccharide complexes produced by mano-thermo-sonication and pH-shifting on the structure and stability of oil-in-water emulsions.Innovative Food Science and Emerging Technologies, 47, 317–325.https://doi.org/10.1016/j.ifset.2018.03.005.
Yildiz, G., Ding, J., Gaur, S., Andrade, J., Engeseth, N. E., & Feng, H. (2018b). Microencapsulation of docosahexaenoic acid (DHA) with four wall materials including pea protein-modified starch complex.International Journal of Biological Macromolecules, 114, 935–941.https://doi.org/10.1016/j.ijbiomac.2018.03.175.
Zhao, J., Wang, H., Zhang, Z., Zhou, X., Yao, J., Zhang, R., Liao, L., & Dong, J. (2019). Vitamin D deficiency as a risk factor for thyroid cancer: a meta-analysis of case-control studies.Nutrition, 57, 5–11.https://doi.org/10.1016/j.nut.2018.04.015.
Acknowledgments
The authors thank Dr. Leon Zhou at the Roquette America for providing pea protein samples.
Funding
This project was partially supported by the Kingdom of Saudi Arabia through the National Science, Technology and Innovation Plan grant 12-NAN2576-02 to AA, HF, TR, JS, and MA.
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Hao Feng
Present address: , Urbana, USA
Authors and Affiliations
Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA
Shanshan Jiang, Junzhou Ding, Juan Andrade & Hao Feng
Food Engineering Department, Faculty of Engineering, Igdir University, Igdir, Turkey
Gulcin Yildiz
Department of Nutrition and Food Technology, Jordan University of Science and Technology, Irbid, Jordan
Taha M. Rababahb
College of Applied Medical Sciences, King Saud University, Riyadh, Saudi Arabia
Ali Almajwalc & Mahmoud M. Abulmeatyc
- Shanshan Jiang
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Jiang, S., Yildiz, G., Ding, J.et al. Pea Protein Nanoemulsion and Nanocomplex as Carriers for Protection of Cholecalciferol (Vitamin D3).Food Bioprocess Technol12, 1031–1040 (2019). https://doi.org/10.1007/s11947-019-02276-0
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