Movatterモバイル変換

[0]ホーム
URL:

Skip to main content
Springer Nature Link
Log in

Expression of interleukin-22 in decidua of patients with early pregnancy and unexplained recurrent pregnancy loss

  • Reproductive Physiology and Disease
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

Researchers have hypothesized that an imbalance of immune cells in the uterine decidua and a dysfunction in cytokines they produce may contribute to recurrent pregnancy loss (RPL). The objective of this study was to determine if IL-22, IL-23 and IL-17 are expressed abnormally in the decidua of patients with RPL compared to those women with a normal pregnancy. We also sought to confirm that uterine natural killer (uNK) cells are lower in the decidua of patients with RPL, as well as identify IL-22 expression by uNK cells.

Methods

After meeting strict inclusion criteria, maternal decidua of nine patients with unexplained RPL and a confirmed euploid fetal loss, and 11 gestational age-matched patients undergoing elective pregnancy termination were included in our analysis. Quantitative real time-polymerase chain reaction (qRT-PCR) was performed to quantify RNA expression, Western blot was performed to quantify protein expression and immunohistochemistry (IHC) was performed to identify IL-22 and uNK cells.

Results

We found that women with unexplained RPL and a euploid fetal loss had significantly less gene and protein expression of IL-22 in the decidua. Additionally, we found that IL-22 is primarily expressed by uNK cells in the decidua.

Conclusions

In conclusion, our results suggest that lower levels of IL-22 in the uterine decidua in patients with unexplained RPL may contribute to a disruption of decidual homeostasis and ultimately lead to early pregnancy loss.

This is a preview of subscription content,log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
¥17,985 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price includes VAT (Japan)

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Jacobs PA, Hassold T. Chromosome abnormalities: origin and etiology in abortions and live births. In: Vogel F, Sperling K, editors. Human genetics. Berlin: Springer; 1987. p. 233–44.

    Chapter  Google Scholar 

  2. Warburton D, Fraser FC. Spontaneous abortion risk in man: data from reproductive histories collected in a medical genetics unit. Am J Hum Genet. 1964;116:1–25.

    Google Scholar 

  3. Stirrat GM. Recurrent miscarriage. Lancet. 1990;336:673–5.

    Article CAS PubMed  Google Scholar 

  4. Suguira-Ogasawara M, Ozaki Y, Katano K, Suzumori N, Kitaori T, Mizutani E. Abnormal embryonic karyotype is the most frequent cause of miscarriage. Hum Reprod. 2012;8:2297–303.

    Article  Google Scholar 

  5. Quack KC, Vassiliadou N, Pudney J, Anderson DJ, Hill JA. Leukocyte activation in the decidua of chromosomally normal and abnormal fetuses from women with recurrent abortion. Hum Reprod. 2001;16:949–55.

    Article CAS PubMed  Google Scholar 

  6. Wang S, Li YP, Ding B, Zhao YR, Chen ZJ, Xu CY, et al. Recurrent miscarriage is associated with a decline of decidual natural killer cells expressing killer cell immunoglobulin-like receptors specific for human leukocyte antigen C. J Obstet Gynaecol Res. 2014;40:1288–95.

    Article CAS PubMed  Google Scholar 

  7. Yamamoto T, Takahashi Y, Kase N, Mori H. Decidual natural killer cells in recurrent spontaneous abortion with normal chromosomal content. Am J Reprod Immunol. 1999;41:337–42.

    Article CAS PubMed  Google Scholar 

  8. Liu YS, Wu L, Tong XH, Wu LM, He GP, Zhou GX, et al. Study on relationship between Th17 cells and unexplained recurrent spontaneous abortion. Am J Reprod Immunol. 2011;65:503–11.

    Article CAS PubMed  Google Scholar 

  9. Wang WJ, Hao CF, Lin Y, Yin GJ, Bao SH, Qiu LH, et al. Increased prevalence of T helper 17 (Th17) cells in peripheral blood and decidua in unexplained recurrent spontaneous abortion patients. J Reprod Immunol. 2010;84:164–70.

    Article CAS PubMed  Google Scholar 

  10. Sabat R, Ouyang W, Wolk K. Therapeutic opportunites of the IL-22-IL-22R1 system. Nat Rev. 2014;13:21–38.

    CAS  Google Scholar 

  11. Wolk K, Kunz S, Witte E, Friedrich M, Asadullah K, Sabat R. IL-22 increases the innate immunity of tissues. Immunity. 2004;21:241–51.

    Article CAS PubMed  Google Scholar 

  12. Wang Y, Xu B, Li MQ, Li DJ, Jin LP. IL-22 secreted by decidual stromal cells and NK cells promotes the survival of human trophoblasts. Int J Clin Exp Pathol. 2013;6:1781–90.

    PubMed Central PubMed  Google Scholar 

  13. Croxford A, Mair F, Becher B. IL-23: one cytokine in control of autoimmunity. Eur J Immunol. 2012;42:2263–73.

    Article CAS PubMed  Google Scholar 

  14. Dumoutier L, Louahed J, Renauld JC. Cloning and characterization of IL-10-related T cell-derived inducible factor (IL-TIF), a novel cytokine structurally related to IL-10 and inducible by IL-9. J Immunol. 2000;164:1814–9.

    Article CAS PubMed  Google Scholar 

  15. Male V, Hughes T, McClory S, Colucci F, Caligiur M, Moffett A. Immature NK cells, capable of producing IL-22, are present in human uterine mucosa. J Immunol. 2010;185:3913–8.

    Article CAS PubMed Central PubMed  Google Scholar 

  16. The Practice Committee of the American Society of Reproductive Medicine. Evaluation and treatment of recurrent pregnancy loss: a committee opinion. Fertil Steril. 2012;98:1103–11.

    Article  Google Scholar 

  17. Cella M, Fuchs A, Vermi W, Facchetti F, Otero K, Lennerz JKM, et al. A human natural killer cell subset provides an innate source of IL-22 for mucosal immunity. Nature. 2009;457:722–5.

    Article CAS PubMed Central PubMed  Google Scholar 

  18. Hanash AM, Dudakov JA, Hua G, O’Connor MH, Young LF, Singer NV, et al. Interleukin-22 protects intestinal stem cells from immune-mediated tissue damage and regulates sensitivity to graft versus host disease. Immunity. 2012;37(2):339–50.

    Article CAS PubMed Central PubMed  Google Scholar 

  19. Zenewicz LA, Yancopoulos GD, Valenzuela DM, Murphy AJ, Stevens S, Flavell RA. Innate and adaptive interleukin-22 protects mice from inflammatory bowel disease. Immunity. 2008;29(6):947–57.

    Article CAS PubMed Central PubMed  Google Scholar 

Download references

Acknowledgments

This research was made possible through the support of the Ernest and Amelia Gallo Endowed Postdoctoral fellowship, the Child Health Research Institute, Lucile Packard Foundation for Children’s Health, as well as the Stanford CTSA (grant number UL1 TR000093).

Conflict of interest

There are no known conflicts of interest associated with this publication.

Author information

Authors and Affiliations

  1. Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Stanford University Medical Center, 900 Welch Rd, Suite 20, Palo Alto, CA, 94304, USA

    Candice O’Hern Perfetto, Xiujun Fan, Sabita Dahl, Sacha Krieg, Lynn Marie Westphal, Ruth Bunker Lathi & Nihar R. Nayak

Authors
  1. Candice O’Hern Perfetto

    You can also search for this author inPubMed Google Scholar

  2. Xiujun Fan

    You can also search for this author inPubMed Google Scholar

  3. Sabita Dahl

    You can also search for this author inPubMed Google Scholar

  4. Sacha Krieg

    You can also search for this author inPubMed Google Scholar

  5. Lynn Marie Westphal

    You can also search for this author inPubMed Google Scholar

  6. Ruth Bunker Lathi

    You can also search for this author inPubMed Google Scholar

  7. Nihar R. Nayak

    You can also search for this author inPubMed Google Scholar

Corresponding author

Correspondence toCandice O’Hern Perfetto.

Additional information

Capsule In patients with unexplained RPL and a euploid fetal loss, lower levels of IL-22 in the decidua may contribute to alterations in decidual homeostasis and play a role in early pregnancy loss.

Rights and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Perfetto, C.O., Fan, X., Dahl, S.et al. Expression of interleukin-22 in decidua of patients with early pregnancy and unexplained recurrent pregnancy loss.J Assist Reprod Genet32, 977–984 (2015). https://doi.org/10.1007/s10815-015-0481-7

Download citation

Keywords

Access this article

Subscribe and save

Springer+ Basic
¥17,985 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price includes VAT (Japan)

Instant access to the full article PDF.

Advertisement

[8]ページ先頭
©2009-2025 Movatter.jp