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Cytokine expression profile in human pancreatic carcinoma cells and in surgical specimens: implications for survival

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Abstract

Cytokine shedding by tumor cells into the local microenvironment modulates host immune response, tumor growth, and metastasis. The study aimed to verify the hypothesis that the immunological microenvironment of pancreatic carcinoma exists in a prevalently immunosuppressive state, influencing survival. We analyzed expression profiles of pro-inflammatory (IL-1β, IL-2, IL-6, IL-8, IL-12 p40, IL-18 and IFN-γ) and anti-inflammatory (IL-10, IL-11, IL-13 and TGF-β isoforms) cytokines. The study was performed both in vitro, in five pancreatic carcinoma cell lines (real time RT-PCR), and in specimens from 65 patients, comparing tumoral versus non-tumoral pancreatic tissues (real time RT-PCR and immunohistochemistry). Furthermore, cytokines were measured in supernatants and sera (from patients and controls) by ELISA. All cell lines expressed IL-8, IL-18, TGF-β1, TGF-β2 and TGF-β3, but not IFN-γ and IL-2 transcripts. Expression of IL-1β, IL-6, IL-10, IL-11, IL-13 and IL-12 mRNA was variable. All the above cytokines were detected as soluble proteins in supernatants, except IL-13. Tumor tissues overexpressed IL-1β, IL-6, IL-8, IL-10, IL-11, IL-12 p40, IL-18, IFN-γ, TGF-β1, TGF-β2 and TGF-β3 at the mRNA level and IL-1β, IL-18, TGF-β2 and TGF-β3 also at the protein level. Conversely, non-tumor tissues had stronger RNA and protein expression of IL-13. Survival was significantly longer in patients with high IL-1β and IL-11 and moderate IL-12 expression. Serum IL-8, IL-10, IL-12, IL-18, TGF-β1 and TGF-β2 were higher in patients than in controls, as opposed to IL-1β and IL-13. Patients with low circulating levels of IL-6, IL-18 and TGF-β2 survived longer. Pancreatic cancer is characterized by peculiar cytokine expression patterns, associated with different survival probabilities.

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Acknowledgments

This work was supported by a grant from MIUR (Rome, Italy) (ex-60%) to GE, and in part by a grant from the Piedmontese Regional Government (Regione Piemonte) to GB. ET and AB are the recipients of an award from the Piedmontese Regional Government.

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Authors and Affiliations

  1. Department of Clinical Physiopathology, Università di Torino, Via Genova, 3, 10126, Torino, Italy

    Graziella Bellone, Carlo Smirne, Elena Tonel, Anna Carbone, Alessandra Buffolino & Giorgio Emanuelli

  2. Department of Medical-Surgical Disciplines, University of Turin, Turin, Italy

    Antonio Robecchi

  3. Medical Sciences, University of Eastern Piedmont, Novara, Italy

    Carlo Smirne & Mario Pirisi

  4. Gastroenterology and Clinical Nutrition, S. Giovanni Battista Hospital, Turin, Italy

    Anna Carbone, Luca Dughera & Giorgio Emanuelli

  5. Histopathology, Hammersmith Hospital, London, UK

    Francesco Angelo Mauri

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  1. Graziella Bellone

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  2. Carlo Smirne

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  3. Francesco Angelo Mauri

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  4. Elena Tonel

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  5. Anna Carbone

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  6. Alessandra Buffolino

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  8. Antonio Robecchi

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  9. Mario Pirisi

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  10. Giorgio Emanuelli

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Correspondence toGraziella Bellone.

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Bellone, G., Smirne, C., Mauri, F.A.et al. Cytokine expression profile in human pancreatic carcinoma cells and in surgical specimens: implications for survival.Cancer Immunol Immunother55, 684–698 (2006). https://doi.org/10.1007/s00262-005-0047-0

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