Movatterモバイル変換

[0]ホーム
URL:

Skip to main content
Springer Nature Link
Log in

Organization of a repetitive human 1.8 kb KpnI sequence localized in the heterochromatin of chromosome 15

  • Published:
Chromosoma Aims and scope Submit manuscript

Abstract

We have isolated a repetitive 1.8 kb Kpnl DNA sequence which is amplified in the homogeneously staining regions of a human melanoma cell line. Under low stringency conditions this sequence (D15Z1) hybridized in situ to the centromeric heterochromatin of chromosomes 1, 9, 15p, 16, and distal Yq as well as to the the short arms of the other acrocentric chromosomes. Under conditions of high stringency, labelling was predominantly on the short arm of chromosome 15. D15Z1 was shown to be present at approximately 3,000 copies per haploid genome and organized in long tandem arrays showing restriction site heterogeneity. Sequences homologous to D15Z1 were highly enriched in the less dense shoulder region of a Ag+—Cs2SO4 gradient. Analysis of D15Z1 indicated that this sequence is composed of tandemly arranged imperfect repeats of the consensus 5′ AATGG 3′ similar to previously identified satellite III sequences. Digestion of D15Z1 with HinfI resulted in a series of restriction fragments making up a subset of the HinfI ladder components of satellites III and IV. These data suggest that D15Z1 represents a chromosome 15 specific domain of human satellites III or IV and that it makes up the major fraction of the heterochromatin of this chromosome. Possible relationships between this sequence and the cytochemical staining properties of human chromosomes with distamycin A/DAPI, D280/170, and antiserum to 5-methylcytosine are discussed.

This is a preview of subscription content,log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
¥17,985 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price includes VAT (Japan)

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Adams JW, Kaufman RE, Kretschmer PJ, Harrison M, Wienhuis AW (1980) A family of long reiterated DNA sequences, one copy of which is next to the human beta globin gene. Nucleic Acids Res 8:6113–6128

    PubMed  Google Scholar 

  • Beauchamp RS, Mitchell AR, Buckland RA, Bostock CJ (1979) Specific arrangements of human satellite III DNA sequences in human chromosomes. Chromosoma 71:153–166

    PubMed  Google Scholar 

  • Blumenfeld M (1973) The evolution of satellite DNA in Drosophila virilis. Cold Spring Harbor Symp Quant Biol 38:423–427

    Google Scholar 

  • Bostock CJ, Gosden JR, Mitchell AR (1978) Localization of a male-specific DNA fragment to a sub-region of the human Y chromosome. Nature 272:324–328

    PubMed  Google Scholar 

  • Brutlag DL (1980) Molecular arrangement and evolution of heterochromatic DNA. Annu Rev Genet 14:121–144

    PubMed  Google Scholar 

  • Clewell DB, Helinski DR (1969) Super-coiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an open circular DNA form. Proc Natl Acad Sci USA 62:1159–1166

    PubMed  Google Scholar 

  • Cooke HJ, Hindley J (1979) Cloning of human satellite III: different components are on different chromosomes. Nucleic Acids Res 6:3177–3197

    PubMed  Google Scholar 

  • Corneo G, Ginelli E, Polli E (1967) A satellite DNA from human tissue. J Mol Biol 23:619–622

    PubMed  Google Scholar 

  • Corneo G, Ginelli E, Polli E (1970) Repeated sequences in human DNA. J Mol Biol 48:319–327

    PubMed  Google Scholar 

  • Corneo G, Ginelli E, Polli E (1971) Renaturation properties and localization in heterochromatin of human satellite DNAs. Biochim Biophys Acta 247:528–534

    PubMed  Google Scholar 

  • Corneo G, Zardi L, Polli E (1972) Evolution of human satellite DNAs on a methylated albumin kieselguhr chromatographic column: Isolation of satellite DNA IV. Biochim Biophys Acta 269:201–204

    PubMed  Google Scholar 

  • Corneo G, Nelli LC, Meazza D, Ginelli E (1980) Repeated nucleotide sequences in human main band DNA. Biochim Biophys Acta 607:438–444

    PubMed  Google Scholar 

  • Deininger PL, Jolly DJ, Rubin CM, Friesman T, Schmid CW (1981) Base sequence studies of 300 nucleotide renatured repeated human DNA clones. J Mol Biol 151:17–33

    PubMed  Google Scholar 

  • Doskocil J, Sorm F (1962) Distribution of 5-methylcytosine in pyrimidine sequences in deoxyribonucleic acids. Biochim Biophys Acta 55:953–959

    PubMed  Google Scholar 

  • Frommer M, Prosser J, Ikachuk D, Reisner AH, Vincent PC (1982) Simple repeated sequences in human satellite DNA. Nucleic Acids Res 10:547–563

    PubMed  Google Scholar 

  • Gall JG, Atherton DD (1974) Satellite DNA sequences in Drosophila virilis. J Mol Biol 85:633–664

    PubMed  Google Scholar 

  • Gillespie D, Adams JW, Costanzi C, Caranfa MJ (1982) New orientations of ancestral “long interspersed repeated sequences” (LINES) in human DNA. Gene 20:409–414

    PubMed  Google Scholar 

  • Gosden JR, Mitchell AR, Buckland RA, Clayton RP, Evans HJ (1975) The location of four human satellite DNAs on human chromosomes. Exp Cell Res 92:148–158

    PubMed  Google Scholar 

  • Gosden JR, Lawrie SS, Cooke HJ (1981b) A cloned repetitive DNA sequence in human chromosome heteromorphisms. Cytogenet Cell Genet 29:32–39

    PubMed  Google Scholar 

  • Gosden JR, Lawrie SS, Gosden CM (1981a) Satellite DNA sequences in human acrocentric chromosomes: information from translocations and heteromorphisms. Am J Hum Genet 33:243–251

    PubMed  Google Scholar 

  • Harper ME, Saunders GF (1981) Localization of single copy DNA sequences on G-banded human chromosomes by in situ hybridization. Chromosoma 83:431–439

    PubMed  Google Scholar 

  • Holden JJA, Reimer DL, Higgins MJ, Roder JC, White BN (1985) Amplified sequences from chromosome 15, including centromeres, nucleolar organizing regions, and centromeric heterochromatin, in homogeneously staining regions in the human melanoma cell line, MeWo. Cancer Genet Cytogenet 14:131–146

    PubMed  Google Scholar 

  • Jeanpierre M, Weil D, Creau-Goldberg N, Gallano P, Turleau C, Kaplan JC (1984) Organization of two subfamilies of a human tandemly repeated DNA is chromosome specific. Cytogenet Cell Genet 37:496

    Google Scholar 

  • Lubit BW, Duc Pham T, Miller OJ, Erlanger BF (1976) Localization of 5-methylcytosine in human metaphase chromosomes by immunoelectron microscopy. Cell 9:503–509

    PubMed  Google Scholar 

  • Maio JJ, Brown FL, McKenna WG, Musich PR (1981) Toward a molecular paleontology of primate genomes. Chromosoma 83:127–144

    PubMed  Google Scholar 

  • Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, NY

    Google Scholar 

  • Manuelidis L (1978) Chromosomal localization of complex and simple repeated human DNAs. Chromosoma 66:23–32

    PubMed  Google Scholar 

  • Miklos GLG, John B (1979) Heterochromatin and satellite DNA in man: properties and prospects. Am J Hum Genet 31:264–280

    PubMed  Google Scholar 

  • Miller OJ, Schnedl W, Allen J, Erlanger BF (1974) 5-methylcytosine localized in mammalian constitutive heterochromatin. Nature 251:636–637

    PubMed  Google Scholar 

  • Mitchell AR, Beauchamp RS, Bostock CJ (1979) A study of sequence homologies in four satellite DNAs of man. J Mol Biol 135:127–149

    PubMed  Google Scholar 

  • Prosser J, Reisner AH, Bradley ML, Ho K, Vincent PC (1981) Buoyant density and hybridization analysis of human DNA sequences, including three satellite DNAs. Biochim Biophys Acta 656:93–102

    PubMed  Google Scholar 

  • Rigby PWJ, Dieckmann M, Rhodes C, Berg P (1977) Labelling deoxribonucleic acid to a high sepcific activity in vitro by nick translation with DNA polymerase. J Mol Biol 113:237–251

    PubMed  Google Scholar 

  • Schmid M, Grunert D, Haaf T, Engel W (1983) A direct demonstration of somatically paired heterochromatin of human chromosomes. Cytogenet Cell Genet 36:554–561

    PubMed  Google Scholar 

  • Schnedl W, Abraham R, Geber G, Schweizer D (1981) Preferential fluorescent staining of heterochromatic regions in human chromosomes 9, 15 and the Y by D287/170. Hum Genet 59:10–13

    PubMed  Google Scholar 

  • Schweizer D, Ambros P, Andrle M (1978) Modification of DAPI banding on human chromosomes by prestaining with DNA-binding oligopeptide antibiotic, distamycin A. Exp Cell Res 111:327–332

    PubMed  Google Scholar 

  • Shafit-Zagardo B, Maio JJ, Brown FL (1982a) KpnI families of long, interspersed repetitive DNAs in human and other primate genomes. Nucleic Acids Res 10:3175–3193

    PubMed  Google Scholar 

  • Shafit-Zagardo B, Brown FL, Maio JJ, Adams JW (1982b) KpnI families of long, interspersed repetitive DNAs associated with the human beta-globin gene cluster. Gene 20:397–407

    PubMed  Google Scholar 

  • Simmons MC, Maxwell J, Halliotis T, Higgins MJ, Roder JC, White BN, Holden JJA (1984) Amplified KpnI repetitive DNA sequences in homogeneously staining regions of a human melanoma cell line. J Natl Cancer Inst 72:801–808

    PubMed  Google Scholar 

  • Singer M (1982) Highly repeated sequences in mammalian genomes: Int Rev Cytol 76:67–117

    PubMed  Google Scholar 

  • Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517

    PubMed  Google Scholar 

  • Wang HS, Riddell DC, Donald LJ, Cameron EC, Tonogai J, Holden JJA, Higgins MJ, Shtromas I, White BN, Hamerton JL (1984) Mapping of a 1.8 kb KpnI sequence to the short arm of chromosome 15. Cytogenet Cell Genet 37:601–602

    Google Scholar 

  • White BA, Bancroft FC (1982) Cytoplasmic dot hybridization: simple analysis of relative mRNA levels in multiple small cell or tissue samples. J Biol Chem 257:8569–8572

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biology, Queen's University, K7L 3N6, Kingston, Ontario, Canada

    Michael J. Higgins, Inna Shtromas, Jeanette J. A. Holden & Bradley N. White

  2. Division of Human Genetics, Department of Paediatrics, The University of Manitoba, R3E OW3, Winnipeg, Manitoba, Canada

    Hungshu Wang

  3. Department of Microbiology and Immunology, Queen's University, K7L 3N6, Kingston, Ontario, Canada

    Tina Haliotis & John C. Roder

  4. Department of Paediatrics, Division of Medical Genetics, Queen's University, K7L 3N6, Kingston, Ontario, Canada

    Jeanette J. A. Holden & Bradley N. White

  5. Cytogenetics Laboratory, Kingston General Hospital, K7L 2V7, Kingston, Ontario, Canada

    Jeanette J. A. Holden

Authors
  1. Michael J. Higgins

    You can also search for this author inPubMed Google Scholar

  2. Hungshu Wang

    You can also search for this author inPubMed Google Scholar

  3. Inna Shtromas

    You can also search for this author inPubMed Google Scholar

  4. Tina Haliotis

    You can also search for this author inPubMed Google Scholar

  5. John C. Roder

    You can also search for this author inPubMed Google Scholar

  6. Jeanette J. A. Holden

    You can also search for this author inPubMed Google Scholar

  7. Bradley N. White

    You can also search for this author inPubMed Google Scholar

About this article

Access this article

Subscribe and save

Springer+ Basic
¥17,985 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price includes VAT (Japan)

Instant access to the full article PDF.

Advertisement

[8]ページ先頭
©2009-2025 Movatter.jp