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Isolation of theERG2 gene, encoding sterol Δ8Δ→7 isomerase, from the rice blast fungusMagnaporthe grisea and its expression in the maize smut pathogenUstilago maydis

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Abstract

TheMagnaporthe grisea ERG2 gene, encoding Δ8Δ→7 sterol isomerase, was isolated from a genomic library by heterologous hybridization to a fragment of theUstilago maydis ERG2 gene. The isolated gene contained a reading frame of 745 bp which encoded a protein of 221 amino acids. The coding region was interrupted by a single putative 79-bp-long intron. The deduced amino-acid sequence exhibited similarity to theERG2 gene products ofU. maydis and ofSaccharomyces cerevisiae, particularly in the central region of the proteins. The NH2-terminal of all three proteins contained a long stretch of amino acids that were strongly hydrophobic, suggesting that they may function by anchoring the protein to a membrane surface. TheM. grisea ERG2 gene complemented aU. maydis deletion mutant in which theERG2 gene had been removed using a one-step gene replacement procedure. The Δ8Δ→7 sterol isomerase produced by theM. grisea ERG2 gene exhibited a level of sensitivity to the sterol biosynthesis inhibitor, tridemorph, similar to that of the enzyme derived from theU. maydis ERG2 gene.

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Authors and Affiliations

  1. Department of Agricultural Sciences, University of Bristol, AFRC Institute of Arable Crops Research, Long Ashton Research Station, BS18 9AF, Bristol, UK

    John P. R. Keon, Caroline S. James, Samantha Court, Raymond S. Burden & John A. Hargreaves

  2. Department of Biological Sciences, University of the West of England, BS16 1QY, Bristol, UK

    Catharine Baden-Daintree

  3. School of Biological Sciences, University of Bath, BA2 7AY, Bath, UK

    Andrew M. Bailey

  4. Department of Biology, Purdue University, 46202, Indianapolis, IN, USA

    Martin Bard

Authors
  1. John P. R. Keon

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  2. Caroline S. James

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  3. Samantha Court

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  4. Catharine Baden-Daintree

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  5. Andrew M. Bailey

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  6. Raymond S. Burden

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  7. Martin Bard

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  8. John A. Hargreaves

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Communicated by C. J. Leaver

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Keon, J.P.R., James, C.S., Court, S.et al. Isolation of theERG2 gene, encoding sterol Δ8Δ→7 isomerase, from the rice blast fungusMagnaporthe grisea and its expression in the maize smut pathogenUstilago maydis.Curr Genet25, 531–537 (1994). https://doi.org/10.1007/BF00351674

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