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Summary
Temperature-sensitive mutants of anEscherichia coli K-12 strain PA3092 have been isolated following mutagenesis with nitrosoguanidine, and their ribosomal proteins analyzed by two-dimensional gel electrophoresis This method was found to be very efficient in obtaining mutants with various structural alterations in ribosomal proteins. Thus a total of some 160 mutants with alterations in 41 different ribosomal proteins have so far been isolated. By characterizing these mutants, we could isolate, not only those mutants with alterations in the structural genes for various ribosomal proteins, but also those with impairments in the modification of proteins S5, S18 and L12. Furthermore, a mutant has been obtained which apparently lacks the protein S20 (L26) with a concomitant reduction to a great extent of the polypeptide synthetic activity of the small subunit. The usefulness of these mutants in establishing the genetic architecture of the genes coding for the ribosomal proteins and their modifiers is discussed.
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Gray, P.N., Bellemare, G., Monier, R., Garrett, R.A., Stöffler, G.: Identification of the nucleotide sequences involved in the interaction betweenEscherichia coli 5S RNA and specific 50S subunit proteins. J. molec. Biol.77, 133–152 (1973)
Hamel, E., Koka, M., Nakamoto, T.: Requirement of anEscherichia coli 50S ribosomal protein component for effective interaction of the ribosome with T and G factors and with guanosine triphosphate. J. biol. Chem.247, 805–814 (1972)
Hardy, S.J.S., Kurland, C.G., Voynow, P., Mora, G.: The ribosomal proteins ofEscherichia coli. I. Purification of the 30S ribosomal proteins. Biochemistry8, 2897–2905 (1969)
Isono, K., Cumberlidge, A.G., Isono, S., Hirota, Y.: Further temperature-sensitive mutants ofEscherichea coli with altered ribosomal proteins. Molec. gen. Genet.152, 239–243 (1977)
Isono, K., Krauss, J., Hirota, Y.: Isolation and characterization of temperature-sensitive mutants ofEscherichia coli with altered ribosomal proteins. Molec. gen. Genet.149, 297–302 (1976)
Lindahl, L., Jaskunas, S.R., Dennis, P.P., Nomura, M.: Cluster of genes inEscherichia coli for ribosomal proteins, ribosomal RNA and RNA polymerase subunits. Proc. nat. Acad. Sci. (Wash.)72, 2743–2747 (1975)
Nomura, M., Held, W.: Reconstitution of ribosomes: Studies of ribosome structure, function and assembly, pp. 193–223. In: Ribosomes (M. Nomura, A. Tissières, and P. Lengyel, eds.). Cold Spring Harbor, New York: Cold Spring Harbor Laboratory 1974
Nomura, M., Morgan, E.A., Jaskunas, S.R.: Genetics of bacterial ribosomes. Ann. Rev. Genet.11, 297–347 (1977)
Pettersson, I., Hardy, S.J.S., Liljas, A.: The ribosomal protein L8 is a complex of L7/L12 and L10. FEBS Lett.64, 135–138 (1976)
Subramanian, A.R.: Copies of proteins L7 and L12 and heterogeneity of the large subunit ofEscherichia coli ribosome. J. molec. Biol.95, 1–8 (1975)
Takata, R.: Genetic studies of the ribosomal proteins inEscherichia coli XI. Mapping of the genes for L21, L27, S15 and S21 by using hybrid bacteria and over-production of these proteins in the merodiploid strains. Molec. gen. Genet.160, 151–155 (1978)
Watson, R.J., Parker, J., Fiil, N.P., Flaks, J.G., Friesen, J.D.: New chromosomal location for structural genes of ribosomal proteins. Proc. nat. Acad. Sci. (Wash.)72, 2765–2769 (1975)
Weber, H.J.: Stoichiometric measurements of 30S and 50S ribosomal proteins from Escherichia coli. Molec. gen. Genet.119, 233–248 (1972)
Wittmann, H.G., Stöffler, G., Hindennach, I., Kurland, C.G., Randall-Hazelbauer, L., Birge, E.A., Nomura, M., Kaltschmidt, E., Mizushima, S., Traut, R.R., Bickle, T.A.: Correlation of 30S ribosomal proteins ofEscherichia coli isolated in different laboratories. Molec. gen. Genet.111, 327–333 (1971)
Yaguchi, M.: Primary structure of protein S18 from the smallEscherichia coli ribosomal subunit. FEBS Lett.59, 217–220 (1975)
Zimmermann, R.A.: RNA-protein interactions in the ribosome, pp. 225–269. In: Ribosomes. (M. Nomura, A. Tissières, and P. Lengyel, eds.). Cold Spring Harbor, New York: Cold Spring Harbor Laboratory 1974
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Abt. Wittmann, Max-Planck-Institut für Molekulare Genetik, Ihnestr 63-73, D-1000, Berlin 33
Setsuko Isono & Katsumi Isono
National Institute of Genetics, Yata, Mishima, Japan
Yukinori Hirota
- Setsuko Isono
Search author on:PubMed Google Scholar
- Katsumi Isono
Search author on:PubMed Google Scholar
- Yukinori Hirota
Search author on:PubMed Google Scholar
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Communicated by E. Bautz
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Isono, S., Isono, K. & Hirota, Y. Mutations affecting the structural genes and the genes coding for modifying enzymes for ribosomal proteins inEscherichia coli.Molec. Gen. Genet.165, 15–20 (1978). https://doi.org/10.1007/BF00270371
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