Movatterモバイル変換


[0]ホーム

URL:


Skip to main content
Springer Nature Link
Log in

Mutations affecting the structural genes and the genes coding for modifying enzymes for ribosomal proteins inEscherichia coli

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

Temperature-sensitive mutants of anEscherichia coli K-12 strain PA3092 have been isolated following mutagenesis with nitrosoguanidine, and their ribosomal proteins analyzed by two-dimensional gel electrophoresis This method was found to be very efficient in obtaining mutants with various structural alterations in ribosomal proteins. Thus a total of some 160 mutants with alterations in 41 different ribosomal proteins have so far been isolated. By characterizing these mutants, we could isolate, not only those mutants with alterations in the structural genes for various ribosomal proteins, but also those with impairments in the modification of proteins S5, S18 and L12. Furthermore, a mutant has been obtained which apparently lacks the protein S20 (L26) with a concomitant reduction to a great extent of the polypeptide synthetic activity of the small subunit. The usefulness of these mutants in establishing the genetic architecture of the genes coding for the ribosomal proteins and their modifiers is discussed.

This is a preview of subscription content,log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+
from ¥17,985 /Month
  • Starting from 10 chapters or articles per month
  • Access and download chapters and articles from more than 300k books and 2,500 journals
  • Cancel anytime
View plans

Buy Now

Price includes VAT (Japan)

Instant access to the full article PDF.

Similar content being viewed by others

Explore related subjects

Discover the latest articles, books and news in related subjects, suggested using machine learning.

References

  • Gray, P.N., Bellemare, G., Monier, R., Garrett, R.A., Stöffler, G.: Identification of the nucleotide sequences involved in the interaction betweenEscherichia coli 5S RNA and specific 50S subunit proteins. J. molec. Biol.77, 133–152 (1973)

    Google Scholar 

  • Hamel, E., Koka, M., Nakamoto, T.: Requirement of anEscherichia coli 50S ribosomal protein component for effective interaction of the ribosome with T and G factors and with guanosine triphosphate. J. biol. Chem.247, 805–814 (1972)

    Google Scholar 

  • Hardy, S.J.S., Kurland, C.G., Voynow, P., Mora, G.: The ribosomal proteins ofEscherichia coli. I. Purification of the 30S ribosomal proteins. Biochemistry8, 2897–2905 (1969)

    Google Scholar 

  • Isono, K., Cumberlidge, A.G., Isono, S., Hirota, Y.: Further temperature-sensitive mutants ofEscherichea coli with altered ribosomal proteins. Molec. gen. Genet.152, 239–243 (1977)

    Google Scholar 

  • Isono, K., Krauss, J., Hirota, Y.: Isolation and characterization of temperature-sensitive mutants ofEscherichia coli with altered ribosomal proteins. Molec. gen. Genet.149, 297–302 (1976)

    Google Scholar 

  • Lindahl, L., Jaskunas, S.R., Dennis, P.P., Nomura, M.: Cluster of genes inEscherichia coli for ribosomal proteins, ribosomal RNA and RNA polymerase subunits. Proc. nat. Acad. Sci. (Wash.)72, 2743–2747 (1975)

    Google Scholar 

  • Nomura, M., Held, W.: Reconstitution of ribosomes: Studies of ribosome structure, function and assembly, pp. 193–223. In: Ribosomes (M. Nomura, A. Tissières, and P. Lengyel, eds.). Cold Spring Harbor, New York: Cold Spring Harbor Laboratory 1974

    Google Scholar 

  • Nomura, M., Morgan, E.A., Jaskunas, S.R.: Genetics of bacterial ribosomes. Ann. Rev. Genet.11, 297–347 (1977)

    Google Scholar 

  • Pettersson, I., Hardy, S.J.S., Liljas, A.: The ribosomal protein L8 is a complex of L7/L12 and L10. FEBS Lett.64, 135–138 (1976)

    Google Scholar 

  • Subramanian, A.R.: Copies of proteins L7 and L12 and heterogeneity of the large subunit ofEscherichia coli ribosome. J. molec. Biol.95, 1–8 (1975)

    Google Scholar 

  • Takata, R.: Genetic studies of the ribosomal proteins inEscherichia coli XI. Mapping of the genes for L21, L27, S15 and S21 by using hybrid bacteria and over-production of these proteins in the merodiploid strains. Molec. gen. Genet.160, 151–155 (1978)

    Google Scholar 

  • Watson, R.J., Parker, J., Fiil, N.P., Flaks, J.G., Friesen, J.D.: New chromosomal location for structural genes of ribosomal proteins. Proc. nat. Acad. Sci. (Wash.)72, 2765–2769 (1975)

    Google Scholar 

  • Weber, H.J.: Stoichiometric measurements of 30S and 50S ribosomal proteins from Escherichia coli. Molec. gen. Genet.119, 233–248 (1972)

    Google Scholar 

  • Wittmann, H.G., Stöffler, G., Hindennach, I., Kurland, C.G., Randall-Hazelbauer, L., Birge, E.A., Nomura, M., Kaltschmidt, E., Mizushima, S., Traut, R.R., Bickle, T.A.: Correlation of 30S ribosomal proteins ofEscherichia coli isolated in different laboratories. Molec. gen. Genet.111, 327–333 (1971)

    Google Scholar 

  • Yaguchi, M.: Primary structure of protein S18 from the smallEscherichia coli ribosomal subunit. FEBS Lett.59, 217–220 (1975)

    Google Scholar 

  • Zimmermann, R.A.: RNA-protein interactions in the ribosome, pp. 225–269. In: Ribosomes. (M. Nomura, A. Tissières, and P. Lengyel, eds.). Cold Spring Harbor, New York: Cold Spring Harbor Laboratory 1974

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Abt. Wittmann, Max-Planck-Institut für Molekulare Genetik, Ihnestr 63-73, D-1000, Berlin 33

    Setsuko Isono & Katsumi Isono

  2. National Institute of Genetics, Yata, Mishima, Japan

    Yukinori Hirota

Authors
  1. Setsuko Isono
  2. Katsumi Isono
  3. Yukinori Hirota

Additional information

Communicated by E. Bautz

Rights and permissions

About this article

Cite this article

Isono, S., Isono, K. & Hirota, Y. Mutations affecting the structural genes and the genes coding for modifying enzymes for ribosomal proteins inEscherichia coli.Molec. Gen. Genet.165, 15–20 (1978). https://doi.org/10.1007/BF00270371

Download citation

Keywords

Access this article

Subscribe and save

Springer+
from ¥17,985 /Month
  • Starting from 10 chapters or articles per month
  • Access and download chapters and articles from more than 300k books and 2,500 journals
  • Cancel anytime
View plans

Buy Now

Price includes VAT (Japan)

Instant access to the full article PDF.

Advertisement


[8]ページ先頭

©2009-2025 Movatter.jp