Part of the book series:Methods in Molecular Biology ((MIMB,volume 1014))
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Abstract
The potential of cell therapy for the regeneration of diseased and damaged tissues is now widely recognized. As a consequence there is a demand for the development of novel systems that can deliver cells to a particular location, maintaining viability, and then degrade at a predictable rate to release the cells into the surrounding tissues. Hydrogels have attracted much attention in this area, as the hydrogel structure provides an environment that is akin to that of the extracellular matrix. One widely investigated hydrogel is alginate, which has been used for cell encapsulation for more than 30 years. Alginate gels have the potential to be used as 3D cell culture systems and as prosthetic materials, both are applied to regeneration of the cornea. Here, we describe an alginate-based process that has been used for encapsulation of mammalian cells including corneal cells, with high levels of viability, and which allows subsequent retrieval of cell cultures for further characterization.
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Acknowledgments
The work reported in this book chapter was funded by the EU (FP6 NanoBioTact and FP7 NanoBioTouch). The authors would like to thank Michele Marigo (University of Birmingham) for the preparation of Fig.1.
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Authors and Affiliations
School of Chemical Engineering, University of Birmingham, Birmingham, UK
Nicola C. Hunt & Liam M. Grover
- Nicola C. Hunt
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- Liam M. Grover
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Editors and Affiliations
School of Pharmacy, University of Reading, Shinfield Road, Reading, RG6 6UB, United Kingdom
Bernice Wright
School of Pharmacy, University of Reading, Shinfield Road, Reading, RG6 6UB, United Kingdom
Che J Connon
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Hunt, N.C., Grover, L.M. (2013). Encapsulation and Culture of Mammalian Cells Including Corneal Cells in Alginate Hydrogels. In: Wright, B., Connon, C. (eds) Corneal Regenerative Medicine. Methods in Molecular Biology, vol 1014. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-432-6_14
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Publisher Name:Humana Press, Totowa, NJ
Print ISBN:978-1-62703-431-9
Online ISBN:978-1-62703-432-6
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