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Abstract
Sixteen polymorphic microsatellite markers were developed from a partial genomic library enriched using AG and AC repeats in mud snail (Bullacta exarata). Polymorphism of these loci was screened using 48 adult individuals ofB. exarata. The number of alleles per locus ranged from 2 to 13. The observed and expected heterozygosities ranged from 0.5106 to 0.8125 and from 0.4868 to 0.9208, respectively. One locus departed significantly from Hardy–Weinberg equilibrium after Bonferroni correction. No linkage disequilibrium was found between any pair of loci. These polymorphic microsatellite markers are expected to provide valuable genetic information in population structure and genetic diversity, which will certainly facilitate management strategies of the mud snail.
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Acknowledgments
This work was supported by funds from National Nonprofit Special Grant of China (nyhyzx07-047) and the National Program for Sci-Tech Basic Conditions Platform Construction (2005DKA30470), and by the earmarked fund for Modern Agro-industry Technology Research System, and in Part by E-Institute of Shanghai Municipal Education Commission (E03009).
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Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, 266003, Qingdao, China
Huixia Du, Wei Peng, Jingjie Hu & Zhenmin Bao
College of Marine Life Sciences, Ocean University of China, 266003, Qingdao, China
Luyang Sun
- Huixia Du
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- Luyang Sun
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- Wei Peng
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- Jingjie Hu
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- Zhenmin Bao
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Correspondence toJingjie Hu.
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Du, H., Sun, L., Peng, W.et al. Sixteen polymorphic microsatellite markers for the mud snail,Bullacta exarata (Philippi, 1848).Conservation Genet Resour2 (Suppl 1), 23–25 (2010). https://doi.org/10.1007/s12686-009-9137-1
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