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Abstract
Rotaviruses are segmented double-stranded RNA viruses with a high frequency of gene reassortment, and they are a leading cause of global diarrheal deaths in children less than 5 years old. Two-thirds of rotavirus-associated deaths occur in low-income countries. Currently, the available vaccines in developing countries have lower efficacy in children than those in developed countries. Due to added safety concerns and the high cost of current vaccines, there is a need to develop cost-effective next-generation vaccines with improved safety and efficacy. The reverse genetics system (RGS) is a powerful tool for investigating viral protein functions and developing novel vaccines. Recently, an entirely plasmid-based RGS has been developed for several rotaviruses, and this technological advancement has significantly facilitated novel rotavirus research. Here, we review the recently developed RGS platform and discuss its application in studying infection biology, gene reassortment, and development of vaccines against rotavirus disease.
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Acknowledgements
We thank all of the members in our laboratory for reading the manuscript and suggesting corrections.
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This work was supported in part by the Agricultural Experiment Station of the University of Kentucky and the William Robert Mills Endowment Fund.
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Department of Veterinary Science, M. H. Gluck Equine Research Center, University of Kentucky, Lexington, KY, 40546, USA
Tirth Uprety, Dan Wang & Feng Li
- Tirth Uprety
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Correspondence toFeng Li.
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Uprety, T., Wang, D. & Li, F. Recent advances in rotavirus reverse genetics and its utilization in basic research and vaccine development.Arch Virol166, 2369–2386 (2021). https://doi.org/10.1007/s00705-021-05142-7
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