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Mycorrhization betweenCistus ladanifer L. andBoletus edulis Bull is enhanced by the mycorrhiza helper bacteriaPseudomonas fluorescens Migula

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Abstract

Boletus edulis Bull. is one of the most economically and gastronomically valuable fungi worldwide. Sporocarp production normally occurs when symbiotically associated with a number of tree species in stands over 40 years old, but it has also been reported in 3-year-oldCistus ladanifer L. shrubs. Efforts toward the domestication ofB. edulis have thus focused on successfully generatingC. ladanifer seedlings associated withB. edulis under controlled conditions. Microorganisms have an important role mediating mycorrhizal symbiosis, such as some bacteria species which enhance mycorrhiza formation (mycorrhiza helper bacteria). Thus, in this study, we explored the effect that mycorrhiza helper bacteria have on the efficiency and intensity of the ectomycorrhizal symbiosis betweenC. ladanifer andB. edulis. The aim of this work was to optimize an in vitro protocol for the mycorrhizal synthesis ofB. edulis withC. ladanifer by testing the effects of fungal culture time and coinoculation with the helper bacteriaPseudomonas fluorescens Migula. The results confirmed successful mycorrhizal synthesis betweenC. ladanifer andB. edulis. Coinoculation ofB. edulis withP. fluorescens doubled within-plant mycorrhization levels although it did not result in an increased number of seedlings colonized withB. edulis mycorrhizae.B. edulis mycelium culture time also increased mycorrhization levels but not the presence of mycorrhizae. These findings bring us closer to controlledB. edulis sporocarp production in plantations.

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Acknowledgments

This study was partially funded by the research project VA206U13 (Junta de Castilla y León). We would like to thank Dr. Valentin Pando (Department of Statistics, University of Valladolid) for the statistical support. We would also like to thank Alfonso Centeno (University of Valladolid), Fernando Fernández (Director of Ecology and Environmental Consultants Ireland Ltd.), and María Hernández Rodríguez (PhD Student, University of Valladolid) for helping to significantly improve the document.

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Authors and Affiliations

  1. Sustainable Forest Management Research Institute, Fire and Applied Mycology Laboratory, Departments of Agroforestry Sciences and Vegetal Production and Natural Resources, University of Valladolid (Palencia), Avda, Madrid 44, 34071, Palencia, Spain

    Olaya Mediavilla, Luis Santos-del-Blanco, Juan Andrés Oria-de-Rueda & Pablo Martín-Pinto

  2. IDForest-Biotecnología Forestal Aplicada, Calle Tren Ter, s/n. Parcela 238, 34200, Venta de Baños, Palencia, Spain

    Olaya Mediavilla & Jaime Olaizola

  3. Department of Ecology and Evolution, Univeristy of Lausanne, CH-1015, Lausanne, Switzerland

    Luis Santos-del-Blanco

Authors
  1. Olaya Mediavilla
  2. Jaime Olaizola
  3. Luis Santos-del-Blanco
  4. Juan Andrés Oria-de-Rueda
  5. Pablo Martín-Pinto

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Correspondence toPablo Martín-Pinto.

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Mediavilla, O., Olaizola, J., Santos-del-Blanco, L.et al. Mycorrhization betweenCistus ladanifer L. andBoletus edulis Bull is enhanced by the mycorrhiza helper bacteriaPseudomonas fluorescens Migula.Mycorrhiza26, 161–168 (2016). https://doi.org/10.1007/s00572-015-0657-0

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