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Occurrence and molecular characterization ofCryptosporidium spp. in mammals and reptiles at the Lisbon Zoo

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Abstract

The presence ofCryptosporidium parasites in mammals and reptiles kept at the Lisbon Zoo was investigated. A total of 274 stool samples were collected from 100 mammals and 29 reptiles. The species and genotype of the isolates identified by light microscopy were determined by nested PCR and sequence analysis of a fragment of the small subunit rRNA gene.Cryptosporidium oocysts were found in one black wildebeest (Connochaetesgnou), one Prairie bison (Bisonbisonbison) and in one Indian star tortoise (Geocheloneelegans). The PCR and sequence analysis of these three isolates showed that those excreted by the Prairie bison wereCryptosporidium mouse genotype, those from the black wildebeest were from a newCryptosporidium genotype and those infecting the Indian star tortoise wereCryptosporidium tortoise genotype. The present work reports a newCryptosporidium genotype in a black wildebeest and the first finding of theCryptosporidium mouse genotype in a ruminant.

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References

  • Alves M, Matos O, Fonseca IP, Delgado E, Lourenço AM, Antunes F (2001) Multilocus genotyping ofCryptosporidium isolates from human HIV-infected and animal hosts. J Euk Microbiol (Suppl):17S-18S

    Google Scholar 

  • Alves M, Xiao L, Sulaiman I, Lal AA, Matos O, Antunes F (2003) Subgenotype analysis ofCryptosporidium isolates from humans, cattle, and zoo ruminants in Portugal. J Clin Microbiol 41:2744–2747

    Article CAS PubMed  Google Scholar 

  • Bajer A, Cacciò S, Bednarska M, Behnke JM, Pieniazek NJ, Sinski E (2003) Preliminary molecular characterization ofCryptosporidium parvum isolates of wildlife rodents from Poland. J Parasitol 89:1053–1055

    CAS PubMed  Google Scholar 

  • Delgado E, Fonseca IP, Fazendeiro I, Matos O, Antunes F, Cunha MB (2003)Cryptosporidium spp. in ruminants at the Lisbon Zoo. J Zoo Wildl Med 34:352–356

    PubMed  Google Scholar 

  • Gómez MS, Torres J, Gracenea M, Fernandez-Morán J, Gonzalez-Moreno O (2000) Further report onCryptosporidium in Barcelona Zoo. Parasitol Res 86:318–323

    Article PubMed  Google Scholar 

  • Graczyk TK, Cranfield MR (2000)Cryptosporidium serpentis oocysts and microsporidian spores in feces of captive snakes. J Parasitol 86:413–414

    Article CAS PubMed  Google Scholar 

  • Graczyk TK, Balazs GH, Work T, Aguirre AA, Ellis DM, Murakawa SKK, Morris R (1997)Cryptosporidium sp. infections in green turtles,Chelonia mydas, as a potential source of marine waterborne occysts in the Hawaiian Islands. Appl Environ Microbiol 63:2925–2927

    CAS  Google Scholar 

  • Majewska AC, Kasprzak W, Werner A (1997) Prevalence ofCryptosporidium in mammals housed in Poznań Zoological Garden, Poland. Acta Parasitol 42:195–198

    Google Scholar 

  • Morgan UM, Xiao L, Fayer R, Lal AA, Thompson RCA (1999a) Variation inCryptosporidium: towards a taxonomic revision of the genus. Int J Parasitol 29:1733–1751

    Article CAS  Google Scholar 

  • Morgan UM, Strudee AP, Singleton G, Gómez MS, Gracenea M, Torres J, Hamilton SG, Woodside DP, Thompson RCA (1999b) TheCryptosporidium “mouse” genotype is conserved across geographic areas. J Clin Microbiol 37:1302–1305

    CAS  Google Scholar 

  • Mtambo MMA, Sebatwale JB, Kambarage DM, Muhairwa AP, Maeda GE, Kusiluka LJM, Kazwala RR (1997) Prevalence ofCryptosporidium spp. oocysts in cattle and wildlife in Morogoro region, Tanzania. Prev Vet Med 31:185–190

    Article CAS PubMed  Google Scholar 

  • Muriuki SMK, Farah IO, Kagwiria RM, Chai DC, Njamunge G, Suleman M, Olobo JO (1997) The presence ofCryptosporidium oocysts in stools of clinically diarrhoeic and normal nonhuman primates in Kenya. Vet Parasitol 72:141–147

    Article CAS PubMed  Google Scholar 

  • Ryan UM, Monis P, Enemark HL, Sulaiman I, Samarasinghe B, Read C, Buddle R, Robertson I, Zhou L, Thompson RC, Xiao L (2004)Cryptosporidiumsuis n. sp. (Apicomplexa: Cryptosporidiidae) in pigs (Susscrofa). J Parasitol 90:769–773

    CAS PubMed  Google Scholar 

  • Xiao L, Ryan UM (2004)Cryptosporidiosis: an update in molecular epidemiology. Curr Opin Infect Dis 17:483–490

    Article PubMed  Google Scholar 

  • Xiao L, Morgan UM, Limor J, Escalante A, Arrowood M, Shulaw W, Thompson RCA, Fayer R, Lal AA (1999) Genetic diversity withinCryptosporidiumparvum and relatedCryptosporidium species. Appl Environ Microbiol 65:3386–3391

    CAS PubMed  Google Scholar 

  • Xiao L, Alderisio K, Limor J, Royer M, Lal AA (2000) Identification of species and sources ofCryptosporidiumoocysts in storm waters with a small-subunit rRNA-based diagnostic and genotyping tool. Appl Environ Microbiol 66:5492–5498

    Article CAS PubMed  Google Scholar 

  • Xiao L, Bern C, Limor J, Sulaiman I, Roberts J, Checkley W, Cabrera L, Gilman RH, Lal AA (2001) Identification of 5 types ofCryptosporidium parasites in children in Lima, Peru. J Infect Dis 183:492–497

    Article CAS PubMed  Google Scholar 

  • Xiao L, Fayer R, Ryan U, Upton SJ (2004a)Cryptosporidium taxonomy: recent advances and implications for public health. Clin Microbiol Rev 17:72–97

    Article  Google Scholar 

  • Xiao L, Ryan UM, Graczyk TK, Limor J, Li L, Kombert M, Junge R, Sulaiman IM, Zhou L, Arrowood MJ, Koudela B, Modrý D, Lal AA (2004b) Genetic diversity ofCryptosporidium spp. in captive reptiles. Appl Environ Microbiol 70:409–417

    Article  Google Scholar 

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Acknowledgements

This work was supported by the projects POCTI/ESP/43635/99 and POCTI/ESP/46369/2002 from the Fundação para a Ciência e Tecnologia (FCT). Margarida Alves received a PhD thesis grant from FCT (SFRH/BD/2898/200). The authors would like to thank the workers of the Lisbon Zoo for their cooperation in sample collection.

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Authors and Affiliations

  1. Unidade de Protozoàrios Oportunistas/VIH e Outras Protozooses, Unidade de Parasitologia e Microbiologia Médicas (UPMM), Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Rua da Junqueira n° 96, 1349-008, Lisboa, Portugal

    Margarida Alves, Vanessa Lemos, Olga Matos & Francisco Antunes

  2. Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, 30341, USA

    Lihua Xiao, Ling Zhou & Vitaliano Cama

  3. Jardim Zoológico de Lisboa, Estrada de Benfica, 158-160, 1549-004, Lisboa, Portugal

    Margarida Barão da Cunha

  4. Clínica Universitária de Doenças Infecciosas, Faculdade de Medicina (Hospital de Santa Maria), Universidade de Lisboa, Av. Egas Moniz, 1649-035, Lisboa, Portugal

    Francisco Antunes

Authors
  1. Margarida Alves
  2. Lihua Xiao
  3. Vanessa Lemos
  4. Ling Zhou
  5. Vitaliano Cama
  6. Margarida Barão da Cunha
  7. Olga Matos
  8. Francisco Antunes

Corresponding author

Correspondence toOlga Matos.

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Alves, M., Xiao, L., Lemos, V.et al. Occurrence and molecular characterization ofCryptosporidium spp. in mammals and reptiles at the Lisbon Zoo.Parasitol Res97, 108–112 (2005). https://doi.org/10.1007/s00436-005-1384-9

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