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Abstract
The lymphoid and immunohaematopoietic tissues of the embryonic and full-term brushtail possums was investigated histologically and immunohistochemically using antibodies to the T- and B-cell markers, CD3, CD5, CD79a and CD79b. No clearly defined thymus, bone marrow, spleen, lymph nodes, gut-associated lymphoid tissues or bronchus-associated lymphoid tissues were observed histologically. The liver was haematopoietic and contained erythrocytic and granulocytic precursors. No mature lymphocytes were observed histologically or detected using antibodies to T- and B-cell markers in any of the tissues. These results are consistent with other studies of the early postnatal tissues of other marsupials and support the proposition that neonatal marsupials are substantially reliant on maternal immunological protection at the time of birth and for a significant period of pouch life.
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References
Adamski FM, Demmer J (1999) Two stages of increased IgA transfer during lactation in the marsupial, Trichosurus vulpecula (brushtail possum). J Immunol 162:6009–6015
Ashman R, Papadimitriou J (1975) Development of the lymphoid tissue in a marsupial, S. brachyurus. Acta Anat (Basel) 91:594–611
Baker ML, Gemmell E, Gemmell RT (1999) Ontogeny of the brushtail possum, Trichosurus vulpecula. Anat Rec 256:354–365
Bancroft JD, Stevens AS (1982) Theory and practice of histological techniques, 2nd edn. Churchill Livingstone, New York
Basden K, Cooper DW, Deane EM (1996) Development of the blood-forming tissues of the tammar wallaby Macropus eugenii. Reprod Fertil Dev 8:989–994
Block M (1964) The blood forming tissues of the newborn opossum (Didelphys virginiana). I. Normal development through about the one hundredth day of life. Ergeb Anat Entwicklungsgesch 37:1–237
Bryant BJ, Shifrine M (1974) The immunohaematopoietic and lymphatic systems of Marmosa mitis: a developmental survey. J Reticuloendothelial Soc 16:105–113
Buaboocha W, Gemmell RT (1997) Development of lung, kidney and skin in the brushtail possum, Trichosurus vulpecula. Acta Anat (Basel) 159:15–24
Cockson A, McNeice R (1980) Survival in the pouch: the role of macrophages and maternal milk cells. Comp Biochem Physiol 66:221–225
Cowan PE, Tyndale-Biscoe CH (1997) Australian and New Zealand mammal species considered to be pests or problems. Reprod Fertil Dev 9:27–36
Cutts H, Krause WJ (1982) Postnatal development of the spleen in Didelphis virginiana. J Anat 135:601–613
Cutts JH, Leeson CR, Krause WJ (1973) The postnatal development of the liver in a marsupial, Didelphis virginiana. 1. Light microscopy. J Anat 115:327–346
Deane EM, Cooper DW (1984) Immunology of pouch young marsupials. I. Levels of immunoglobulin transferrin and albumin in the blood and milk of euros and wallaroos (Hill kangaroos: Macropus robustus, Marsupialia). Dev Comp Immunol 8:863–876
Deane EM, Cooper DW, Renfree MB (1990) Immunoglobulin G levels in fetal and newborn tammar wallabies (Macropus eugenii). Reprod Fert Dev 2:369–375
Gemmell RT (1986) Lung development in the marsupial bandicoot, Isoodon macrourus. J Anat 148:193–204
Gemmell RT, Little GJ (1982) The structure of the lung of the newborn marsupial bandicoot, Isoodon macrourus. Cell Tissue Res 223:445–453
Gemmell RT, Selwood L (1994) Structural development in the newborn marsupial, the striped-faced dunnart, Sminthopsis macroura. Acta Anat (Basel) 149:1–12
Hemsley SW, Canfield PJ, Husband AJ (1995) Immunohistological staining of lymphoid tissue in four Australian marsupial species using species cross-reactive antibodies. Immunol Cell Biol 73:321–325
Heuser CH (1921) The early establishment of the intestinal nutrition in the opossum: the digestive system just before and soon after birth. Am J Anat 28:341–356
Hughes RL, Hall LS (1984) Embryonic development in the common brush-tail possum Trichosurus vulpecula. In: Smith AP, Hume ID (eds) Possums and gliders. Surrey Beatty and the Australian Mammal Society, Sydney, pp 197–212
McCrady E (1938) The embryology of the opossum. Am J Anat 16:1–233
Old JM, Deane EM (1998) The effect of oestrus and the presence of pouch young on aerobic bacteria isolated from the pouch of the tammar wallaby, Macropus eugenii. Comp Immunol Microbiol Infect Dis 21:237–245
Old JM, Deane EM (2000) Development of the immune system and immunological protection in marsupial pouch young. Dev Comp Immunol 24:445–454
Yadav M (1972) Characteristics of blood in the pouch young of a marsupial, Setonix brachyurus. Aust J Zool 20:249–263
Young LJ, Deane EM (2001) Cellular composition of the late milk of the koala (Phascolarctos cinereus). Aust J Zool 49:195–202
Young L, Basden K, Cooper D, Deane E (1997) Cellular components of the milk of the tammar wallaby (Macropus eugenii). Aust J Zool 45:423–433
Acknowledgements
This study was conducted with the assistance of a University of Western Sydney, Nepean Summer scholarship award to J.M.O. and a PhD scholarship to J.M.O. from the CRC for Marsupial Conservation and Management. Samples were kindly donated by Dr. Terry Fletcher and Dr. Janine Duckworth from the CRC for Marsupial Conservation and Management. Antibodies were kindly donated by Dr. Margaret Jones of the Immunodiagnostic Unit, Radcliffe Hospital, Oxford. We would like to acknowledge and thank Anne Peck and Caroline Wilson for technical assistance.
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Division of Environmental and Life Sciences, Macquarie University, 2109, N.S.W., Australia
E. M. Deane
Cooperative Research Centre for the Conservation and Management of Marsupials, Sydney, N.S.W., Australia
J. M. Old & E. M. Deane
Department of Biological Sciences, Division of Environmental and Life Science, Macquarie University, 2109, N.S.W., Australia
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Old, J.M., Deane, E.M. The lymphoid and immunohaematopoietic tissues of the embryonic brushtail possum (Trichosurus vulpecula).Anat Embryol206, 193–197 (2003). https://doi.org/10.1007/s00429-002-0285-2
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