- Fredd Vergara ORCID:orcid.org/0000-0002-7397-20921,
- Misao Itouga2,
- Roberto Gamboa Becerra3,
- Masami Hirai1,
- José Juan Ordaz-Ortiz3 &
- …
- Robert Winkler4
623Accesses
7Altmetric
Abstract
Main conclusion
The method introduced here to growF. hygrometrica in high concentrations of D2O is an excellent alternative to produce highly deuterated metabolites with broad applications in metabolic studies. Our mass spectrometry experiments strongly indicate the successful incorporation of deuterium into organic compounds.
Deuterated metabolites are useful tracers for metabolic studies, yet their wide utilization in research is limited by the multi-step total synthesis required to produce them in the laboratory. Alternatively, deuterated metabolites can be obtained from organisms grown in D2O or deuterated nutrients. This approach also has limitations as D2O in high concentrations negatively affects the survival of most organisms. Here we report the mossFunaria hygrometrica as an unusual high tolerant to D2O in liquid culture. We found that this moss is able to grow in up to 90% D2O, a condition lethal for many eukaryotes. Mass spectrometric analyses ofF.hygrometrica extracts showed a strong deuteration pattern. The ability to tolerate high concentrations of D2O together with the development of a rich molecular toolbox makesF.hygrometrica an ideal system for the production of valuable deuterated metabolites.
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Acknowledgements
This study was supported in part by JSPS KAKENHI Grant number 25113010 to M.Y.H. The financial support was provided by CINVESTAV and CONACYT basic science grant FRONTERAS 2015-2/814 to R.W. The authors are grateful to Esperanza Ayala Gil, M.Sc., and Dr. David Gómez Zepeda for their support on mass spectrometry analysis.
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Authors and Affiliations
Metabolic Systems Research Team, RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan
Fredd Vergara & Masami Hirai
Plant Productivity Systems Research Group, RIKEN Center for Sustainable Resource Science, Tsurumi-ku, Yokohama, Japan
Misao Itouga
National Laboratory of Genomics for Biodiversity, CINVESTAV Unidad Irapuato, Irapuato, Mexico
Roberto Gamboa Becerra & José Juan Ordaz-Ortiz
Department of Biotechnology and Biochemistry, CINVESTAV Unidad Irapuato, Irapuato, Mexico
Robert Winkler
- Fredd Vergara
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- Misao Itouga
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- Roberto Gamboa Becerra
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- Masami Hirai
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- José Juan Ordaz-Ortiz
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- Robert Winkler
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Correspondence toFredd Vergara.
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Vergara, F., Itouga, M., Becerra, R.G.et al.Funaria hygrometrica Hedw. elevated tolerance to D2O: its use for the production of highly deuterated metabolites.Planta247, 405–412 (2018). https://doi.org/10.1007/s00425-017-2794-5
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