- Jacques Simard1,
- Dominique Bérubé2,
- Märten Sandberg3,
- Karl-Heinz Grzeschik4,
- Richard Gagné2,
- Vidar Hansson3 &
- …
- Tore Jahnsen3
54Accesses
11Citations
3 Altmetric
Summary
A cDNA for the human catalytic subunit (Cβ) of cAMP-dependent protein kinase (PKA) has been cloned from a testis cDNA library. In the present study, we have determined the chromosomal localization of this gene using a cDNA for Cβ as a probe. Southern blot analysis of genomic DNA from human/mouse cell hybrids revealed that the presence or absence of a 20-kbXbaI fragment, which hybridized with the Cβ probe, was concordant with the presence of human chromosome 1.In situ hybridization to metaphase chromosome confirmed the somatic cell hybrid data and regionally mapped the Cβ gene of PKA to the p36 band on chromosome 1.
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Authors and Affiliations
MRC Group in Molecular Endocrinology, CHUL Research Center and Laval University, Québec, Canada
Jacques Simard
Department of Human Genetics, CHUL Research Center and Laval University, Québec
Dominique Bérubé & Richard Gagné
Institute of Medical Biochemistry and Laboratory for Gene Technology, Institute of Pathology, Rikshospitalet, University of Oslo, Blindern, P.B. 1112, N-0317, Oslo 3, Norway
Märten Sandberg, Vidar Hansson & Tore Jahnsen
Institut für Human Genetik der Universität, Bahnhofstrasse 7A, W-3550, Marburg, Germany
Karl-Heinz Grzeschik
- Jacques Simard
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- Dominique Bérubé
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- Märten Sandberg
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- Karl-Heinz Grzeschik
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- Richard Gagné
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- Vidar Hansson
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- Tore Jahnsen
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Simard, J., Bérubé, D., Sandberg, M.et al. Assignment of the gene encoding the catalytic subunit Cβ of CAMP-dependent protein kinase to the p36 band on chromosome.Hum Genet88, 653–657 (1992). https://doi.org/10.1007/BF02265292
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