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Assignment of the gene encoding the catalytic subunit Cβ of CAMP-dependent protein kinase to the p36 band on chromosome

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Summary

A cDNA for the human catalytic subunit (Cβ) of cAMP-dependent protein kinase (PKA) has been cloned from a testis cDNA library. In the present study, we have determined the chromosomal localization of this gene using a cDNA for Cβ as a probe. Southern blot analysis of genomic DNA from human/mouse cell hybrids revealed that the presence or absence of a 20-kbXbaI fragment, which hybridized with the Cβ probe, was concordant with the presence of human chromosome 1.In situ hybridization to metaphase chromosome confirmed the somatic cell hybrid data and regionally mapped the Cβ gene of PKA to the p36 band on chromosome 1.

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Authors and Affiliations

  1. MRC Group in Molecular Endocrinology, CHUL Research Center and Laval University, Québec, Canada

    Jacques Simard

  2. Department of Human Genetics, CHUL Research Center and Laval University, Québec

    Dominique Bérubé & Richard Gagné

  3. Institute of Medical Biochemistry and Laboratory for Gene Technology, Institute of Pathology, Rikshospitalet, University of Oslo, Blindern, P.B. 1112, N-0317, Oslo 3, Norway

    Märten Sandberg, Vidar Hansson & Tore Jahnsen

  4. Institut für Human Genetik der Universität, Bahnhofstrasse 7A, W-3550, Marburg, Germany

    Karl-Heinz Grzeschik

Authors
  1. Jacques Simard
  2. Dominique Bérubé
  3. Märten Sandberg
  4. Karl-Heinz Grzeschik
  5. Richard Gagné
  6. Vidar Hansson
  7. Tore Jahnsen

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Simard, J., Bérubé, D., Sandberg, M.et al. Assignment of the gene encoding the catalytic subunit Cβ of CAMP-dependent protein kinase to the p36 band on chromosome.Hum Genet88, 653–657 (1992). https://doi.org/10.1007/BF02265292

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