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Fluorescencein situ hybridization analysis of chromosomal localization of three human cytochrome P450 2C genes (CYP2C8, 2C9, and 2C10) at 10q24.1
- Kiyoshi Inoue1,
- Johji Inazawa2,
- Yasuhiko Suzuki1,
- Tsutomu Shimada1,
- Hiroshi Yamazaki1,
- F. Peter Guengerich3 &
- …
- Tatsuo Abe2
Japanese journal of human geneticsvolume 39, pages337–343 (1994)Cite this article
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Summary
Chromosomal localization of three human cytochrome P450 genes belonging to the CYP2C subfamily (CYP2C8, 2C9, and 2C10) was identified by fluorescencein situ hybridization (FISH). An original MP-8 clone was used as a DNA probe for the assignment of the CYP2C10 gene, while two cDNA probes, a 1.37 kb fragment of CYP2C8 and a 1.19 kb fragment (MP-20 and MP-4 clones, respectively) by polymerase chain reaction using a single human liver cDNA library. The results showed that three human CYP2C8, 2C9, and 2C10 cDNAs were located at the same subchromosomal region, 10q24.1.
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Authors and Affiliations
Osaka Prefectural Institute of Public Health, Nakamichi, Higashinari-ku, 537, Osaka, Japan
Kiyoshi Inoue, Yasuhiko Suzuki, Tsutomu Shimada & Hiroshi Yamazaki
Department of Hygiene, Kyoto Prefectural University of Medicine, Kamigyo-ku, 602, Kyoto, Japan
Johji Inazawa & Tatsuo Abe
Department of Biochemistry and Center in Molecular Toxicology, Vanderbilt University School of Medicine, 37232, Nashville, TN, USA
F. Peter Guengerich
- Kiyoshi Inoue
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- Johji Inazawa
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- Yasuhiko Suzuki
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- Tsutomu Shimada
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- Hiroshi Yamazaki
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- F. Peter Guengerich
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- Tatsuo Abe
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Additional information
The coding sequences of the genes termed CYP2C9 and CYP2C10 differ in only two amino acids, 358 and 417 (Gedet al., 1988; Srivastavaet al., 1991). The CYP2C10 sequence corresponds to the first cDNA we isolated from this family (Umbenhaueret al., 1987). We reported that the two cDNAs now termed 2C9 (MP-4) and 2C10 (MP-8) differed considerably in their 3′ non-coding sequences, and oligonucleotide probes were used to identify both groups of sequences in the mRNA of a single liver sample (Gedet al., 1988). It is conceivable that the existence of the two cDNA clones (within an expression library generated from a single individual) is an artifact of the library construction, or that the sequences recognized by the probes are parts of other genes. Nevertheless, P450 2C9 and P450 2C10 are treated here as the products of individual genes. When proteins purified from the liver are considered here, they are designated P450 2C9/10 because no amino acid sequence analysis was done in the regions where differences occur (Gedet al., 1988; Srivastavaet al., 1991).
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Inoue, K., Inazawa, J., Suzuki, Y.et al. Fluorescencein situ hybridization analysis of chromosomal localization of three human cytochrome P450 2C genes (CYP2C8, 2C9, and 2C10) at 10q24.1.Jap J Human Genet39, 337–343 (1994). https://doi.org/10.1007/BF01874052
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