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Effect of environmental factors onFonsecaea pedrosoi morphogenesis with emphasis on sclerotic cells induced by propranolol

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Abstract

The influence of growth conditions, as well as of propranolol onFonsecaea pedrosoi morphogenesis was established using the chemically defined media of Czapeck-Dox (CD) and Butterfield (BF). Mycelial growth ofF. pedrosoi in both media was obtained at room temperature (25 °C) for 14 days, without shaking, whereas conidia formed at 37 °C, for 4 days, in shaken cultures and could be isolated free from the mycelium by filtration in gauze. At low pH (2.5–3.0), there appeared sclerotic cells attached to normal hyphae. When propranolol was added to the CD medium moniliform hyphae were observed, whereas this drug in the BF medium induced formation of sclerotic cells. Ultrastructural examination revealed that the propranolol-induced sclerotic cells were very similar to those observed in infected tissues.

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Authors and Affiliations

  1. Instituto de Microbiologia, Universidade Federal, 21941, Rio de Janeiro, RJ, Brasil

    Celuta S. Alviano & Jayme Angluster

  2. Instituto de Biofisica Carlos Chagas Filho, Universidade Federal, 21941, Rio de Janeiro, RJ, Brasil

    Sonia R. Farbiarz & Wanderley de Souza

  3. Disciplina de Biologia Celular, Escola Paulista de Medicina, 04023, São Paulo, SP, Brasil

    Luiz R. Travassos

Authors
  1. Celuta S. Alviano

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  2. Sonia R. Farbiarz

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  3. Luiz R. Travassos

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  4. Jayme Angluster

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  5. Wanderley de Souza

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Alviano, C.S., Farbiarz, S.R., Travassos, L.R.et al. Effect of environmental factors onFonsecaea pedrosoi morphogenesis with emphasis on sclerotic cells induced by propranolol.Mycopathologia119, 17–23 (1992). https://doi.org/10.1007/BF00492225

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