Klaspin jestprotein koji je kodljudi kodiran genom CLSPN sahromosoma 1 .[ 5] [ 6] [ 7]
Dužinapolipeptidnog lanca je 1.339aminokiselina , amolekulska težina 151.094Da .[ 8]
10 20 30 40 50 MTGEVGSEVH LEINDPNVIS QEEADSPSDS GQGSYETIGP LSEGDSDEEI FVSKKLKNRK VLQDSDSETE DTNASPEKTT YDSAEEENKE NLYAGKNTKI KRIYKTVADS DESYMEKSLY QENLEAQVKP CLELSLQSGN STDFTTDRKS SKKHIHDKEG TAGKAKVKSK RRLEKEERKM EKIRQLKKKE TKNQEDDVEQ PFNDSGCLLV DKDLFETGLE DENNSPLEDE ESLESIRAAV KNKVKKHKKK EPSLESGVHS FEEGSELSKG TTRKERKAAR LSKEALKQLH SETQRLIRES ALNLPYHMPE NKTIHDFFKR KPRPTCHGNA MALLKSSKYQ SSHHKEIIDT ANTTEMNSDH HSKGSEQTTG AENEVETNAL PVVSKETQII TGSDESCRKD LVKNEELEIQ EKQKQSDIRP SPGDSSVLQQ ESNFLGNNHS EECQVGGLVA FEPHALEGEG PQNPEETDEK VEEPEQQNKS SAVGPPEKVR RFTLDRLKQL GVDVSIKPRL GADEDSFVIL EPETNRELEA LKQRFWKHAN PAAKPRAGQT VNVNVIVKDM GTDGKEELKA DVVPVTLAPK KLDGASHTKP GEKLQVLKAK LQEAMKLRRF EERQKRQALF KLDNEDGFEE EEEEEEEMTD ESEEDGEEKV EKEEKEEELE EEEEKEEEEE EEGNQETAEF LLSSEEIETK DEKEMDKENN DGSSEIGKAV GFLSVPKSLS SDSTLLLFKD SSSKMGYFPT EEKSETDENS GKQPSKLDED DSCSLLTKES SHNSSFELIG STIPSYQPCN RQTGRGTSFF PTAGGFRSPS PGLFRASLVS SASKSSGKLS EPSLPIEDSQ DLYNASPEPK TLFLGAGDFQ FCLEDDTQSQ LLDADGFLNV RNHRNQYQAL KPRLPLASMD ENAMDANMDE LLDLCTGKFT SQAEKHLPRK SDKKENMEEL LNLCSGKFTS QDASTPASSE LNKQEKESSM GDPMEEALAL CSGSFPTDKE EEDEEEEFGD FRLVSNDNEF DSDEDEHSDS GNDLALEDHE DDDEEELLKR SEKLKRQMRL RKYLEDEAEV SGSDVGSEDE YDGEEIDEYE EDVIDEVLPS DEELQSQIKK IHMKTMLDDD KRQLRLYQER YLADGDLHSD GPGRMRKFRW KNIDDASQMD LFHRDSDDDQ TEEQLDESEA RWRKERIERE QWLRDMAQQG KITAEEEEEI GEDSQFMILA KKVTAKALQK NASRPMVIQE SKSLLRNPFE AIRPGSAQQV KTGSLLNQPK AVLQKLAALS DHNPSAPRNS RNFVFHTLSP VKAEAAKESS KSQVKKRGPS FMTSPSPKHL KTDDSTSGLT RSIFKYLES
Klaspin u žaba rodaXenopus kinazne kontrolne tačke 1 i pokreće zaustavljanje kontrolne tačkećelijskog ciklusa u prisustvu DNK šablona u ekstraktima ksenopusovih jaja. Čini se da je ljudski genhomolog njegovom klaspinu, a njegova funkcija još nije utvrđena.[ 7]
Pokazalo se da CLSPN imainterakcije sa:
^a b c GRCh38: Ensembl release 89: ENSG00000092853 -Ensembl , maj 2017^a b c GRCm38: Ensembl release 89: ENSMUSG00000042489 -Ensembl , maj 2017^ "Human PubMed Reference:" .National Center for Biotechnology Information, U.S. National Library of Medicine .^ "Mouse PubMed Reference:" .National Center for Biotechnology Information, U.S. National Library of Medicine .^ Kumagai A, Dunphy WG (Nov 2000)."Claspin, a novel protein required for the activation of Chk1 during a DNA replication checkpoint response in Xenopus egg extracts" .Mol Cell .6 (4): 839–49.doi :10.1016/S1097-2765(05)00092-4 PMID 11090622 . ^a b Chini CC, Chen J (Aug 2003)."Human claspin is required for replication checkpoint control" .J Biol Chem .278 (32): 30057–62.doi :10.1074/jbc.M301136200 PMID 12766152 . ^a b "Entrez Gene: CLSPN claspin homolog (Xenopus laevis)" .^ "UniProt, Q9HAW4" (jezik: engleski). Pristupljeno 23. 11. 2021 .^ Lin SY, Li K, Stewart GS, Elledge SJ (2004)."Human Claspin works with BRCA1 to both positively and negatively regulate cell proliferation" .Proc. Natl. Acad. Sci. U.S.A .101 (17): 6484–9.Bibcode :2004PNAS..101.6484L .doi :10.1073/pnas.0401847101 PMC 404071 PMID 15096610 . ^a b c Serçin O, Kemp MG (maj 2011)."Characterization of functional domains in human Claspin" .Cell Cycle .10 (10): 1599–606.doi :10.4161/cc.10.10.15562 .PMC 3127160 PMID 21478680 . ^ Faustrup H, Bekker-Jensen S, Bartek J, Lukas J, Mailand N (2009)."USP7 counteracts SCFbetaTrCP- but not APCCdh1-mediated proteolysis of Claspin" .J. Cell Biol .184 (1): 13–9.doi :10.1083/jcb.200807137 .PMC 2615094 PMID 19124652 . Chini CC, Chen J (2005). "Claspin, a regulator of Chk1 in DNA replication stress pathway".DNA Repair (Amst.) .3 (8–9): 1033–7.doi :10.1016/j.dnarep.2004.03.001 .PMID 15279790 . Strausberg RL, Feingold EA, Grouse LH, et al. (2003)."Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences" .Proc. Natl. Acad. Sci. U.S.A .99 (26): 16899–903.doi :10.1073/pnas.242603899 PMC 139241 PMID 12477932 . Lin SY, Li K, Stewart GS, Elledge SJ (2004)."Human Claspin works with BRCA1 to both positively and negatively regulate cell proliferation" .Proc. Natl. Acad. Sci. U.S.A .101 (17): 6484–9.Bibcode :2004PNAS..101.6484L .doi :10.1073/pnas.0401847101 PMC 404071 PMID 15096610 . Sørensen CS, Syljuåsen RG, Lukas J, Bartek J (2006)."ATR, Claspin and the Rad9-Rad1-Hus1 complex regulate Chk1 and Cdc25A in the absence of DNA damage" .Cell Cycle .3 (7): 941–5.doi :10.4161/cc.3.7.972 PMID 15190204 . Sar F, Lindsey-Boltz LA, Subramanian D, et al. (2004)."Human claspin is a ring-shaped DNA-binding protein with high affinity to branched DNA structures" .J. Biol. Chem .279 (38): 39289–95.doi :10.1074/jbc.M405793200 PMID 15226314 . Gerhard DS, Wagner L, Feingold EA, et al. (2004)."The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)" .Genome Res .14 (10B): 2121–7.doi :10.1101/gr.2596504 .PMC 528928 PMID 15489334 . Clarke CA, Clarke PR (2005)."DNA-dependent phosphorylation of Chk1 and Claspin in a human cell-free system" .Biochem. J .388 (Pt 2): 705–12.doi :10.1042/BJ20041966 .PMC 1138979 PMID 15707391 . Clarke CA, Bennett LN, Clarke PR (2005)."Cleavage of claspin by caspase-7 during apoptosis inhibits the Chk1 pathway" .J. Biol. Chem .280 (42): 35337–45.doi :10.1074/jbc.M506460200 PMID 16123041 . Chini CC, Wood J, Chen J (2006)."Chk1 is required to maintain claspin stability" .Oncogene .25 (30): 4165–71.doi :10.1038/sj.onc.1209447 PMID 16501606 . Gregory SG, Barlow KF, McLay KE, et al. (2006)."The DNA sequence and biological annotation of human chromosome 1" .Nature .441 (7091): 315–21.Bibcode :2006Natur.441..315G .doi :10.1038/nature04727 PMID 16710414 . Bennett LN, Clarke PR (2006)."Regulation of Claspin degradation by the ubiquitin-proteosome pathway during the cell cycle and in response to ATR-dependent checkpoint activation" .FEBS Lett .580 (17): 4176–81.doi :10.1016/j.febslet.2006.06.071 PMID 16828751 . Liu S, Bekker-Jensen S, Mailand N, et al. (2006)."Claspin operates downstream of TopBP1 to direct ATR signaling towards Chk1 activation" .Mol. Cell. Biol .26 (16): 6056–64.doi :10.1128/MCB.00492-06 .PMC 1592810 PMID 16880517 . Mailand N, Bekker-Jensen S, Bartek J, Lukas J (2006)."Destruction of Claspin by SCFbetaTrCP restrains Chk1 activation and facilitates recovery from genotoxic stress" .Mol. Cell .23 (3): 307–18.doi :10.1016/j.molcel.2006.06.016 PMID 16885021 . Peschiaroli A, Dorrello NV, Guardavaccaro D, et al. (2006)."SCFbetaTrCP-mediated degradation of Claspin regulates recovery from the DNA replication checkpoint response" .Mol. Cell .23 (3): 319–29.doi :10.1016/j.molcel.2006.06.013 PMID 16885022 . Mamely I, van Vugt MA, Smits VA, et al. (2006)."Polo-like kinase-1 controls proteasome-dependent degradation of Claspin during checkpoint recovery" .Curr. Biol .16 (19): 1950–5.doi :10.1016/j.cub.2006.08.026 PMID 16934469 . Rodríguez-Bravo V, Guaita-Esteruelas S, Florensa R, et al. (2007)."Chk1- and claspin-dependent but ATR/ATM- and Rad17-independent DNA replication checkpoint response in HeLa cells" .Cancer Res .66 (17): 8672–9.doi :10.1158/0008-5472.CAN-05-4443 PMID 16951182 . Chini CC, Chen J (2006)."Repeated phosphopeptide motifs in human Claspin are phosphorylated by Chk1 and mediate Claspin function" .J. Biol. Chem .281 (44): 33276–82.doi :10.1074/jbc.M604373200 PMID 16963448 . Sercin O, Kemp MG (2011)."Characterization of functional domains in human Claspin" .Cell Cycle .10 (10): 1599–606.doi :10.4161/cc.10.10.15562 .PMC 3127160 PMID 21478680 . 
