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CleanUpRNAseq

This is thereleased version of CleanUpRNAseq; for the devel version, seeCleanUpRNAseq.

Detect and Correct Genomic DNA Contamination in RNA-seq Data

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DOI: 10.18129/B9.bioc.CleanUpRNAseq


Bioconductor version: Release (3.22)

RNA-seq data generated by some library preparation methods, such as rRNA-depletion-based method and the SMART-seq method, might be contaminated by genomic DNA (gDNA), if DNase I disgestion is not performed properly during RNA preparation. CleanUpRNAseq is developed to check if RNA-seq data is suffered from gDNA contamination. If so, it can perform correction for gDNA contamination and reduce false discovery rate of differentially expressed genes.

Author: Haibo Liu [aut, cre]ORCID iD ORCID: 0000-0002-4213-2883, Kevin O'Connor [ctb], Michelle Kelliher [ctb], Lihua Julie Zhu [aut], Kai Hu [aut]

Maintainer: Haibo Liu <haibo.liu at umassmed.edu>

Citation (from within R, entercitation("CleanUpRNAseq")):

Installation

To install this package, start R (version "4.5") and enter:

if (!require("BiocManager", quietly = TRUE))    install.packages("BiocManager")BiocManager::install("CleanUpRNAseq")

For older versions of R, please refer to the appropriateBioconductor release.

Documentation

To view documentation for the version of this package installed in your system, start R and enter:

browseVignettes("CleanUpRNAseq")
CleanUpRNAseq: detecting and correcting for DNA contamination\nin RNA-seq dataHTMLR Script
Reference ManualPDF
NEWSText

Need some help? Ask on the Bioconductor Support site!

Details

biocViewsGeneExpression,QualityControl,Sequencing,Software
Version1.4.0
In Bioconductor sinceBioC 3.20 (R-4.4) (1 year)
LicenseGPL-3
DependsR (>= 4.4.0)
ImportsAnnotationFilter,BiocGenerics,Biostrings,BSgenome,DESeq2,edgeR,ensembldb,Seqinfo,GenomicRanges,ggplot2,ggrepel, graphics, grDevices,KernSmooth,limma, methods,pheatmap,qsmooth,R6,RColorBrewer,Rsamtools,Rsubread,reshape2,SummarizedExperiment, stats,tximport, utils
System Requirements
URL
Bug Reportshttps://github.com/haibol2016/CleanUpRNAseq/issues
See More
SuggestsBiocStyle,BSgenome.Hsapiens.UCSC.hg38,EnsDb.Hsapiens.v86,ggplotify,knitr,patchwork,R.utils,rmarkdown,testthat (>= 3.0.0)
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Depends On Me
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Build ReportBuild Report

Package Archives

FollowInstallation instructions to use this package in your R session.

Source PackageCleanUpRNAseq_1.4.0.tar.gz
Windows Binary (x86_64) CleanUpRNAseq_1.4.0.zip
macOS Binary (x86_64)CleanUpRNAseq_1.4.0.tgz
macOS Binary (arm64)CleanUpRNAseq_1.4.0.tgz
Source Repositorygit clone https://git.bioconductor.org/packages/CleanUpRNAseq
Source Repository (Developer Access)git clone git@git.bioconductor.org:packages/CleanUpRNAseq
Bioc Package Browserhttps://code.bioconductor.org/browse/CleanUpRNAseq/
Package Short Urlhttps://bioconductor.org/packages/CleanUpRNAseq/
Package Downloads ReportDownload Stats

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