Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.
OverviewData source: UniProt
| Official Gene Symbol | Other Aliases |
|---|---|
| Msln | Mes, Mpf |
| Sequence Length (AA) | Molecular Weight (Da) |
|---|---|
| 625 | 69423 |
| Protein Name |
|---|
| Mesothelin |
| Sources | |
|---|---|
| UniProt PhosphoSitePlus ® GeneCards | Human Protein Atlas |
Protein Sequence hover to view complete sequence
CPTAC-6242: View additionalEIDESLIFYK data
CPTAC-6244: View additionalTDAVLPLTVAEVQK data
non-CPTAC-3929: View additionalTFLGGASVK data
| 10 | 20 | 30 | 40 | 50 |
| MALPTARPLL | GSCGSPICSR | SFLLLLLSLG | WIPRLQTQTT | KTSQEATLLH |
| 60 | 70 | 80 | 90 | 100 |
| AVNGAADFAS | LPTGLFLGLT | CEEVSDLSME | QAKGLAMAVR | QKNITLRGHQ |
| 110 | 120 | 130 | 140 | 150 |
| LRCLARRLPR | HLTDEELNAL | PLDLLLFLNP | AMFPGQQACA | HFFSLISKAN |
| 160 | 170 | 180 | 190 | 200 |
| VDVLPRRSLE | RQRLLMEALK | CQGVYGFQVS | EADVRALGGL | ACDLPGKFVA |
| 210 | 220 | 230 | 240 | 250 |
| RSSEVLLPWL | AGCQGPLDQS | QEKAVREVLR | SGRTQYGPPS | KWSVSTLDAL |
| 260 | 270 | 280 | 290 | 300 |
| QSLVAVLDES | IVQSIPKDVK | AEWLQHISRD | PSRLGSKLTV | IHPRFRRDAE |
| 310 | 320 | 330 | 340 | 350 |
| QKACPPGKEP | YKVDEDLIFY | QNWELEACVD | GTMLARQMDL | VNEIPFTYEQ |
| 360 | 370 | 380 | 390 | 400 |
| LSIFKHKLDK | TYPQGYPESL | IQQLGHFFRY | VSPEDIHQWN | VTSPDTVKTL |
| 410 | 420 | 430 | 440 | 450 |
| LKVSKGQKMN | AQAIALVACY | LRGGGQLDED | MVKALGDIPL | SYLCDFSPQD |
| 460 | 470 | 480 | 490 | 500 |
| LHSVPSSVMW | LVGPQDLDKC | SQRHLGLLYQ | KACSAFQNVS | GLEYFEKIKT |
| 510 | 520 | 530 | 540 | 550 |
| FLGGASVKDL | RALSQHNVSM | DIATFKRLQV | DSLVGLSVAE | VQKLLGPNIV |
| 560 | 570 | 580 | 590 | 600 |
| DLKTEEDKSP | VRDWLFRQHQ | KDLDRLGLGL | QGGIPNGYLV | LDFNVREAFS |
| 610 | 620 | 625 | ||
| SRASLLGPGF | VLIWIPALLP | ALRLS |
Data source: UniProt
Protein MapCollapse protein map
Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs
Uniprot Database EntryPhosphoSitePlus ®
Click a point on a node to view detailed assay information below
All other points link out toUniProt
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Assay Details for CPTAC-6242Collapse assay details
Data source: Panorama
- Official Gene Symbol
- MSLN
- Peptide Sequence
- EIDESLIFYK
- Modification Type
- unmodified
- Protein - Site of Modification
- N/A
- Peptide - Site of Modification
- 7
- Peptide Start
- 310
- Peptide End
- 319
- CPTAC ID
- CPTAC-6242
- Peptide Molecular Mass
- 1,255.6336
- Species
- HomoSapiens
- Assay Type
- Enrichment MRM
- Enrichment Method
- IMAC
- Matrix
- Frozen tissue
- Submitting Laboratory
- Fred Hutchinson Cancer Research Center
- Submitting Lab PI
- Amanda Paulovich
Assay ParametersCollapse assay parameters
Data source: Panorama
- Instrument
- QTRAP 5500
- Internal Standard
- Extended heavy peptide
- Peptide Standard Purity
- >95%
- Peptide Standard Label Type
- 13C and 15N at C-terminus K
- LC
- Eksigent
- Column Packing
- Reprosil C18, 3um
- Column Dimensions
- 75µm x 15cm
- Flow Rate
- 0.3 µL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| Average intra-assay CV (within day CV) | Average inter-assay CV (between day CV) | Total CV | n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y8 (1+) | 11.7 | 5.3 | 5.9 | 15.1 | 7.7 | 9.8 | 19.1 | 9.3 | 11.4 | 15 | 15 | 15 |
| y6 (1+) | 7.4 | 7.1 | 4.6 | 7.7 | 6.6 | 9.5 | 10.7 | 9.7 | 10.6 | 15 | 15 | 15 |
| y4 (1+) | 8 | 5.4 | 10.8 | 9.7 | 6 | 10.4 | 12.6 | 8.1 | 15 | 15 | 15 | 15 |
| sum | 7.4 | 4 | 4 | 9.2 | 4.8 | 8 | 11.8 | 6.2 | 8.9 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-6244Collapse assay details
Data source: Panorama
- Official Gene Symbol
- MSLN
- Peptide Sequence
- TDAVLPLTVAEVQK
- Modification Type
- unmodified
- Protein - Site of Modification
- N/A
- Peptide - Site of Modification
- 7
- Peptide Start
- 536
- Peptide End
- 549
- CPTAC ID
- CPTAC-6244
- Peptide Molecular Mass
- 1,482.8294
- Species
- HomoSapiens
- Assay Type
- Enrichment MRM
- Enrichment Method
- IMAC
- Matrix
- Frozen tissue
- Submitting Laboratory
- Fred Hutchinson Cancer Research Center
- Submitting Lab PI
- Amanda Paulovich
Assay ParametersCollapse assay parameters
Data source: Panorama
- Instrument
- QTRAP 5500
- Internal Standard
- Extended heavy peptide
- Peptide Standard Purity
- >95%
- Peptide Standard Label Type
- 13C and 15N at C-terminus K
- LC
- Eksigent
- Column Packing
- Reprosil C18, 3um
- Column Dimensions
- 75µm x 15cm
- Flow Rate
- 0.3 µL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| Average intra-assay CV (within day CV) | Average inter-assay CV (between day CV) | Total CV | n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y10 (1+) | 3.9 | 4.3 | 3 | 8.5 | 4.6 | 3 | 9.4 | 6.3 | 4.2 | 15 | 15 | 15 |
| y9 (1+) | 5.2 | 3.7 | 2.8 | 6.7 | 4.3 | 6.2 | 8.5 | 5.7 | 6.8 | 15 | 15 | 15 |
| b5 (1+) | 6.6 | 3.4 | 2.6 | 7.6 | 4.8 | 5.2 | 10.1 | 5.9 | 5.8 | 15 | 15 | 15 |
| sum | 4.9 | 3.5 | 2.3 | 6.8 | 3.8 | 5.1 | 8.4 | 5.2 | 5.6 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for non-CPTAC-3929Collapse assay details
Data source: Panorama
- Official Gene Symbol
- Msln
- Peptide Sequence
- TFLGGASVK
- Modification Type
- unmodified
- Protein - Site of Modification
- N/A
- Peptide - Site of Modification
- N/A
- Peptide Start
- 500
- Peptide End
- 508
- CPTAC ID
- non-CPTAC-3929
- Peptide Molecular Mass
- 878.4862
- Species
- Musmusculus (Mouse)
- Assay Type
- Direct MRM
- Matrix
- Plasma
- Submitting Laboratory
- UVic-Genome BC Proteomics Centre
- Submitting Lab PI
- Christoph Borchers
Publication
View Details(opens in a new window)
Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.
Assay ParametersCollapse assay parameters
Data source: Panorama
- Instrument
- Agilent 6490/6495 QQQ
- Internal Standard
- synthetic peptide
- Peptide Standard Purity
- >80%
- Peptide Standard Label Type
- 13C and 15N at C-terminus K
- LC
- 1290 LC (Agilent)
- Column Packing
- Zorbax Eclipse Plus C18, 1.8 µm
- Column Dimensions
- 2.1 x 150 mm
- Flow Rate
- 400 µL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| Average intra-assay CV (within day CV) | Average inter-assay CV (between day CV) | Total CV | n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| b3 (1+) | 17.2 | 15.4 | 5.3 | 53.7 | 23.4 | 11.2 | 56.4 | 28 | 12.4 | 15 | 15 | 15 |
| b2 (1+) | 14.2 | 3.4 | 4.8 | 17.9 | 7.6 | 9.6 | 22.8 | 8.3 | 10.7 | 15 | 15 | 15 |
| y4 (2+) | 122.7 | 124.6 | 124.5 | 186 | 117.6 | 123.2 | 222.8 | 171.3 | 175.2 | 15 | 15 | 15 |
| y6 (1+) | 19.9 | 7.8 | 3.8 | 22.4 | 11.9 | 9 | 30 | 14.2 | 9.8 | 15 | 15 | 15 |
| y7 (1+) | 13 | 2.2 | 4.2 | 14.4 | 4.1 | 5.6 | 19.4 | 4.7 | 7 | 15 | 15 | 15 |
| sum | 10.5 | 2.8 | 2.6 | 16.7 | 7.1 | 7 | 19.7 | 7.6 | 7.5 | 15 | 15 | 15 |