Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

OverviewData source: UniProt

Official Gene SymbolOther Aliases
DUTN/A
Sequence Length (AA)Molecular Weight (Da)
25226563
Protein Name
Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial
Sources
UniProt
PhosphoSitePlus ®
GeneCards		Human Protein Atlas

Protein Sequence hover to view complete sequence

CPTAC-65: View additionalGNVGVVLFNFGK data
CPTAC-64: View additionalTDIQIALPSGC[+57.0]YGR data
1020304050
MTPLCPRPALCYHFLTSLLRSAMQNARGARQRAEAAVLSGPGPPLGRAAQ
60708090100
HGIPRPLSSAGRLSQGCRGASTVGAAGWKGELPKAGGSPAPGPETPAISP
110120130140150
SKRARPAEVGGMQLRFARLSEHATAPTRGSARAAGYDLYSAYDYTIPPME
160170180190200
KAVVKTDIQIALPSGCYGRVAPRSGLAAKHFIDVGAGVIDEDYRGNVGVV
210220230240250
LFNFGKEKFEVKKGDRIAQLICERIFYPEIEEVQALDDTERGSGGFGSTG
252
KN

Data source: UniProt

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database EntryPhosphoSitePlus ®

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to view detailed assay information below
All other points link out toUniProt


Phosphorylation Acetylation UbiquitylationOther

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Assay Details for CPTAC-65Collapse assay details

Data source: Panorama

Official Gene Symbol
DUT
Peptide Sequence
GNVGVVLFNFGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
195
Peptide End
206
CPTAC ID
CPTAC-65
Peptide Molecular Mass
1,249.6819
Species
Homosapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details(opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG.Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay ParametersCollapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

 Average intra-assay CV
(within day CV)		Average inter-assay CV
(between day CV)		Total CV
equation		n=
Fragment ion / TransitionLowMedHighLowMedHighLowMedHighLowMedHigh
y9 (1+)10.17.28.712.220.310.515.821.513.6152515
y7 (1+)8.913.16.513.616.9916.321.411.1152515
y6 (1+)7.716.18.613.818.811.315.824.814.2152515
sum5.68.57.111.914.69.513.216.911.9152515

Additional Resources and Comments

Assay Details for CPTAC-64Collapse assay details

Data source: Panorama

Official Gene Symbol
DUT
Peptide Modified Sequence
TDIQIALPSGC[+57.0]YGR
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
166
Peptide - Site of Modification
11
Peptide Start
156
Peptide End
169
CPTAC ID
CPTAC-64
Peptide Molecular Mass
1,549.7559
Species
Homosapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details(opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG.Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay ParametersCollapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

 Average intra-assay CV
(within day CV)		Average inter-assay CV
(between day CV)		Total CV
equation		n=
Fragment ion / TransitionLowMedHighLowMedHighLowMedHighLowMedHigh
y9 (1+)4911.611.454.613.515.773.417.819.4152515
y8 (1+)46.715.18.65313.410.470.620.213.5152515
y7 (1+)31.185.234.38.39.646.311.510.9152515
sum22.58.15.334.79.1941.412.210.4152515

Additional Resources and Comments