Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

OverviewData source: UniProt

Official Gene SymbolOther Aliases
MAN2B1LAMAN, MANB
Sequence Length (AA)Molecular Weight (Da)
1011113744
Protein Name
Lysosomal alpha-mannosidase
Sources
UniProt
PhosphoSitePlus ®
GeneCards		Human Protein Atlas

Protein Sequence hover to view complete sequence

CPTAC-2225: View additionalTN[+1.0]HTVM[+16.0]TM[+16.0]GSDFQYENANM[+16.0]WFK data
non-CPTAC-3591: View additionalGVSEPLLETDTGDK data
1020304050
MGAYARASGVCARGCLDSAGPWTMSRALRPPLPPLCFFLLLLAAAGARAG
60708090100
GYETCPTVQPNMLNVHLLPHTHDDVGWLKTVDQYFYGIKNDIQHAGVQYI
110120130140150
LDSVISALLADPTRRFIYVEIAFFSRWWHQQTNATQEVVRDLVRQGRLEF
160170180190200
ANGGWVMNDEAATHYGAIVDQMTLGLRFLEDTFGNDGRPRVAWHIDPFGH
210220230240250
SREQASLFAQMGFDGFFFGRLDYQDKWVRMQKLEMEQVWRASTSLKPPTA
260270280290300
DLFTGVLPNGYNPPRNLCWDVLCVDQPLVEDPRSPEYNAKELVDYFLNVA
310320330340350
TAQGRYYRTNHTVMTMGSDFQYENANMWFKNLDKLIRLVNAQQAKGSSVH
360370380390400
VLYSTPACYLWELNKANLTWSVKHDDFFPYADGPHQFWTGYFSSRPALKR
410420430440450
YERLSYNFLQVCNQLEALVGLAANVGPYGSGDSAPLNEAMAVLQHHDAVS
460470480490500
GTSRQHVANDYARQLAAGWGPCEVLLSNALARLRGFKDHFTFCQQLNISI
510520530540550
CPLSQTAARFQVIVYNPLGRKVNWMVRLPVSEGVFVVKDPNGRTVPSDVV
560570580590600
IFPSSDSQAHPPELLFSASLPALGFSTYSVAQVPRWKPQARAPQPIPRRS
610620630640650
WSPALTIENEHIRATFDPDTGLLMEIMNMNQQLLLPVRQTFFWYNASIGD
660670680690700
NESDQASGAYIFRPNQQKPLPVSRWAQIHLVKTPLVQEVHQNFSAWCSQV
710720730740750
VRLYPGQRHLELEWSVGPIPVGDTWGKEVISRFDTPLETKGRFYTDSNGR
760770780790800
EILERRRDYRPTWKLNQTEPVAGNYYPVNTRIYITDGNMQLTVLTDRSQG
810820830840850
GSSLRDGSLELMVHRRLLKDDGRGVSEPLMENGSGAWVRGRHLVLLDTAQ
860870880890900
AAAAGHRLLAEQEVLAPQVVLAPGGGAAYNLGAPPRTQFSGLRRDLPPSV
910920930940950
HLLTLASWGPEMVLLRLEHQFAVGEDSGRNLSAPVTLNLRDLFSTFTITR
9609709809901000
LQETTLVANQLREAASRLKWTTNTGPTPHQTPYQLDPANITLEPMEIRTF
10101011
LASVQWKEVDG

Data source: UniProt

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database EntryPhosphoSitePlus ®

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to view detailed assay information below
All other points link out toUniProt


Phosphorylation Acetylation UbiquitylationOther

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Assay Details for CPTAC-2225Collapse assay details

Data source: Panorama

Official Gene Symbol
MAN2B1
Peptide Modified Sequence
TN[+1.0]HTVM[+16.0]TM[+16.0]GSDFQYENANM[+16.0]WFK
Modification Type
Deamidated (N), Oxidation (M), Oxidation (M), Oxidation (M)
Protein - Site of Modification
310, 314, 316, 327
Peptide - Site of Modification
2, 6, 8, 19
Peptide Start
309
Peptide End
330
CPTAC ID
CPTAC-2225
Peptide Molecular Mass
2,700.0931
Species
Homosapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay ParametersCollapse assay parameters

Data source: Panorama

Instrument
LCMS-8040
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Nexera X2 UHPLC
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay MultiplexingExpand assay panel

Johns Hopkins University-G

CPTAC-2204:
AOC1.
LEN[+1.0]ITNPWSPR		CPTAC-2205:
APOB.
FEVDSPVYN[+1.0]ATWSASLK		CPTAC-2206:
ATP1B3.
LHVGYLQPLVAVQVSFAPN[+1.0]NTGK
CPTAC-2207:
ADAMTS12.
VGN[+1.0]WSK		CPTAC-2208:
B3GNT2.
DTFFN[+1.0]LSLK		CPTAC-2209:
BMPR2.
ISHEN[+1.0]GTILC[+57.0]SK
CPTAC-2210:
CADM1.
VDDEM[+16.0]PQHAVLSGPNLFINNLN[+1.0]K		CPTAC-2211:
CDCP1.
ESN[+1.0]ITVLIK		CPTAC-2212:
FBLN5.
N[+1.0]HTC[+57.0]NLQQTC[+57.0]YNLQGGFK
CPTAC-2213:
FGL2.
VAN[+1.0]LTFVVNSLDGK		CPTAC-2214:
FGL2.
LHVGNYN[+1.0]GTAGDALR		CPTAC-2215:
FUCA1.
N[+1.0]TTSVWYTSK
CPTAC-2216:
GALNT5.
AN[+1.0]TSLPFPK		CPTAC-2217:
GGT1.
LHNQLLPN[+1.0]VTTVER		CPTAC-2218:
GNPTAB.
TN[+1.0]VSHVVGQLDDIR
CPTAC-2219:
GNPTG.
YEFC[+57.0]PFHN[+1.0]VTQHEQTFR		CPTAC-2220:
HYOU1.
VFGSQN[+1.0]LTTVK		CPTAC-2221:
IL1RAP.
N[+1.0]TTYC[+57.0]SK
CPTAC-2222:
LRP1.
ELQGN[+1.0]C[+57.0]SR		CPTAC-2223:
LRP1.
DGTC[+57.0]IGN[+1.0]SSR		CPTAC-2224:
GAA.
QVVEN[+1.0]M[+16.0]TR
CPTAC-2225:
MAN2B1.
TN[+1.0]HTVM[+16.0]TM[+16.0]GSDFQYENANM[+16.0]WFK		CPTAC-2226:
MFAP4.
VDLEDFEN[+1.0]NTAYAK		CPTAC-2227:
MUC16.
TSSTEGN[+1.0]FTK
CPTAC-2228:
NAGA.
LGIYADM[+16.0]GN[+1.0]FTC[+57.0]M[+16.0]GYPGTTLDK		CPTAC-2229:
P2RX7.
TTN[+1.0]VSLYPGYNFR		CPTAC-2230:
POGLUT1.
N[+1.0]STAYFR
CPTAC-2231:
GPLD1.
N[+1.0]LTTSLTESVDR		CPTAC-2232:
PLOD1.
YIHQN[+1.0]YTK		CPTAC-2233:
PON1.
HAN[+1.0]WTLTPLK
CPTAC-2234:
LTBR.
AGHFQN[+1.0]TSSPSAR		CPTAC-2235:
TLR8.
GSYPQHIN[+1.0]ISR

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

 Average intra-assay CV
(within day CV)		Average inter-assay CV
(between day CV)		Total CV
equation		n=
Fragment ion / TransitionLowMedHighLowMedHighLowMedHighLowMedHigh
y7 (1+)26.38.210.324.99.31136.212.415.1151515
y8 (1+)2211.67.319.89.56.329.6159.6151515
y9 (1+)19.48.112.222.27.58.429.51114.8151515
sum15.56.27.414.45.26.121.28.19.6151515

Additional Resources and Comments

Assay Details for non-CPTAC-3591Collapse assay details

Data source: Panorama

Official Gene Symbol
Man2b1
Peptide Sequence
GVSEPLLETDTGDK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
825
Peptide End
838
CPTAC ID
non-CPTAC-3591
Peptide Molecular Mass
1,459.7042
Species
Musmusculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details(opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay ParametersCollapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Assay MultiplexingExpand assay panel

Johns Hopkins University-G

CPTAC-2204:
AOC1.
LEN[+1.0]ITNPWSPR		CPTAC-2205:
APOB.
FEVDSPVYN[+1.0]ATWSASLK		CPTAC-2206:
ATP1B3.
LHVGYLQPLVAVQVSFAPN[+1.0]NTGK
CPTAC-2207:
ADAMTS12.
VGN[+1.0]WSK		CPTAC-2208:
B3GNT2.
DTFFN[+1.0]LSLK		CPTAC-2209:
BMPR2.
ISHEN[+1.0]GTILC[+57.0]SK
CPTAC-2210:
CADM1.
VDDEM[+16.0]PQHAVLSGPNLFINNLN[+1.0]K		CPTAC-2211:
CDCP1.
ESN[+1.0]ITVLIK		CPTAC-2212:
FBLN5.
N[+1.0]HTC[+57.0]NLQQTC[+57.0]YNLQGGFK
CPTAC-2213:
FGL2.
VAN[+1.0]LTFVVNSLDGK		CPTAC-2214:
FGL2.
LHVGNYN[+1.0]GTAGDALR		CPTAC-2215:
FUCA1.
N[+1.0]TTSVWYTSK
CPTAC-2216:
GALNT5.
AN[+1.0]TSLPFPK		CPTAC-2217:
GGT1.
LHNQLLPN[+1.0]VTTVER		CPTAC-2218:
GNPTAB.
TN[+1.0]VSHVVGQLDDIR
CPTAC-2219:
GNPTG.
YEFC[+57.0]PFHN[+1.0]VTQHEQTFR		CPTAC-2220:
HYOU1.
VFGSQN[+1.0]LTTVK		CPTAC-2221:
IL1RAP.
N[+1.0]TTYC[+57.0]SK
CPTAC-2222:
LRP1.
ELQGN[+1.0]C[+57.0]SR		CPTAC-2223:
LRP1.
DGTC[+57.0]IGN[+1.0]SSR		CPTAC-2224:
GAA.
QVVEN[+1.0]M[+16.0]TR
CPTAC-2225:
MAN2B1.
TN[+1.0]HTVM[+16.0]TM[+16.0]GSDFQYENANM[+16.0]WFK		CPTAC-2226:
MFAP4.
VDLEDFEN[+1.0]NTAYAK		CPTAC-2227:
MUC16.
TSSTEGN[+1.0]FTK
CPTAC-2228:
NAGA.
LGIYADM[+16.0]GN[+1.0]FTC[+57.0]M[+16.0]GYPGTTLDK		CPTAC-2229:
P2RX7.
TTN[+1.0]VSLYPGYNFR		CPTAC-2230:
POGLUT1.
N[+1.0]STAYFR
CPTAC-2231:
GPLD1.
N[+1.0]LTTSLTESVDR		CPTAC-2232:
PLOD1.
YIHQN[+1.0]YTK		CPTAC-2233:
PON1.
HAN[+1.0]WTLTPLK
CPTAC-2234:
LTBR.
AGHFQN[+1.0]TSSPSAR		CPTAC-2235:
TLR8.
GSYPQHIN[+1.0]ISR

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

 Average intra-assay CV
(within day CV)		Average inter-assay CV
(between day CV)		Total CV
equation		n=
Fragment ion / TransitionLowMedHighLowMedHighLowMedHighLowMedHigh
b4 (1+)195.7517.58.19.925.89.911.1151515
b3 (1+)20.15.33.724.58.11331.79.713.5151515
y10 (2+)10.764.712.78.26.716.610.28.2151515
y8 (1+)9.16.17.48.28.77.512.210.610.5151515
y10 (1+)5.355.27.29.28.28.910.59.7151515
sum4.31.52.66.56.66.97.86.87.4151515

Additional Resources and Comments