Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

OverviewData source: UniProt

Official Gene SymbolOther Aliases
GAAN/A
Sequence Length (AA)Molecular Weight (Da)
952105324
Protein Name
Lysosomal alpha-glucosidase
Sources
UniProt
PhosphoSitePlus ®
GeneCards		Human Protein Atlas

Protein Sequence hover to view complete sequence

CPTAC-2224: View additionalQVVEN[+1.0]M[+16.0]TR data
1020304050
MGVRHPPCSHRLLAVCALVSLATAALLGHILLHDFLLVPRELSGSSPVLE
60708090100
ETHPAHQQGASRPGPRDAQAHPGRPRAVPTQCDVPPNSRFDCAPDKAITQ
110120130140150
EQCEARGCCYIPAKQGLQGAQMGQPWCFFPPSYPSYKLENLSSSEMGYTA
160170180190200
TLTRTTPTFFPKDILTLRLDVMMETENRLHFTIKDPANRRYEVPLETPHV
210220230240250
HSRAPSPLYSVEFSEEPFGVIVRRQLDGRVLLNTTVAPLFFADQFLQLST
260270280290300
SLPSQYITGLAEHLSPLMLSTSWTRITLWNRDLAPTPGANLYGSHPFYLA
310320330340350
LEDGGSAHGVFLLNSNAMDVVLQPSPALSWRSTGGILDVYIFLGPEPKSV
360370380390400
VQQYLDVVGYPFMPPYWGLGFHLCRWGYSSTAITRQVVENMTRAHFPLDV
410420430440450
QWNDLDYMDSRRDFTFNKDGFRDFPAMVQELHQGGRRYMMIVDPAISSSG
460470480490500
PAGSYRPYDEGLRRGVFITNETGQPLIGKVWPGSTAFPDFTNPTALAWWE
510520530540550
DMVAEFHDQVPFDGMWIDMNEPSNFIRGSEDGCPNNELENPPYVPGVVGG
560570580590600
TLQAATICASSHQFLSTHYNLHNLYGLTEAIASHRALVKARGTRPFVISR
610620630640650
STFAGHGRYAGHWTGDVWSSWEQLASSVPEILQFNLLGVPLVGADVCGFL
660670680690700
GNTSEELCVRWTQLGAFYPFMRNHNSLLSLPQEPYSFSEPAQQAMRKALT
710720730740750
LRYALLPHLYTLFHQAHVAGETVARPLFLEFPKDSSTWTVDHQLLWGEAL
760770780790800
LITPVLQAGKAEVTGYFPLGTWYDLQTVPVEALGSLPPPPAAPREPAIHS
810820830840850
EGQWVTLPAPLDTINVHLRAGYIIPLQGPGLTTTESRQQPMALAVALTKG
860870880890900
GEARGELFWDDGESLEVLERGAYTQVIFLARNNTIVNELVRVTSEGAGLQ
910920930940950
LQKVTVLGVATAPQQVLSNGVPVSNFTYSPDTKVLDICVSLLMGEQFLVS
952
WC

Data source: UniProt

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database EntryPhosphoSitePlus ®

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Phosphorylation Acetylation UbiquitylationOther

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Assay Details for CPTAC-2224Collapse assay details

Data source: Panorama

Official Gene Symbol
GAA
Peptide Modified Sequence
QVVEN[+1.0]M[+16.0]TR
Modification Type
Deamidated (N), Oxidation (M)
Protein - Site of Modification
390, 391
Peptide - Site of Modification
5, 6
Peptide Start
386
Peptide End
393
CPTAC ID
CPTAC-2224
Peptide Molecular Mass
992.4597
Species
Homosapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay ParametersCollapse assay parameters

Data source: Panorama

Instrument
LCMS-8040
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Nexera X2 UHPLC
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay MultiplexingExpand assay panel

Johns Hopkins University-G

CPTAC-2204:
AOC1.
LEN[+1.0]ITNPWSPR		CPTAC-2205:
APOB.
FEVDSPVYN[+1.0]ATWSASLK		CPTAC-2206:
ATP1B3.
LHVGYLQPLVAVQVSFAPN[+1.0]NTGK
CPTAC-2207:
ADAMTS12.
VGN[+1.0]WSK		CPTAC-2208:
B3GNT2.
DTFFN[+1.0]LSLK		CPTAC-2209:
BMPR2.
ISHEN[+1.0]GTILC[+57.0]SK
CPTAC-2210:
CADM1.
VDDEM[+16.0]PQHAVLSGPNLFINNLN[+1.0]K		CPTAC-2211:
CDCP1.
ESN[+1.0]ITVLIK		CPTAC-2212:
FBLN5.
N[+1.0]HTC[+57.0]NLQQTC[+57.0]YNLQGGFK
CPTAC-2213:
FGL2.
VAN[+1.0]LTFVVNSLDGK		CPTAC-2214:
FGL2.
LHVGNYN[+1.0]GTAGDALR		CPTAC-2215:
FUCA1.
N[+1.0]TTSVWYTSK
CPTAC-2216:
GALNT5.
AN[+1.0]TSLPFPK		CPTAC-2217:
GGT1.
LHNQLLPN[+1.0]VTTVER		CPTAC-2218:
GNPTAB.
TN[+1.0]VSHVVGQLDDIR
CPTAC-2219:
GNPTG.
YEFC[+57.0]PFHN[+1.0]VTQHEQTFR		CPTAC-2220:
HYOU1.
VFGSQN[+1.0]LTTVK		CPTAC-2221:
IL1RAP.
N[+1.0]TTYC[+57.0]SK
CPTAC-2222:
LRP1.
ELQGN[+1.0]C[+57.0]SR		CPTAC-2223:
LRP1.
DGTC[+57.0]IGN[+1.0]SSR		CPTAC-2224:
GAA.
QVVEN[+1.0]M[+16.0]TR
CPTAC-2225:
MAN2B1.
TN[+1.0]HTVM[+16.0]TM[+16.0]GSDFQYENANM[+16.0]WFK		CPTAC-2226:
MFAP4.
VDLEDFEN[+1.0]NTAYAK		CPTAC-2227:
MUC16.
TSSTEGN[+1.0]FTK
CPTAC-2228:
NAGA.
LGIYADM[+16.0]GN[+1.0]FTC[+57.0]M[+16.0]GYPGTTLDK		CPTAC-2229:
P2RX7.
TTN[+1.0]VSLYPGYNFR		CPTAC-2230:
POGLUT1.
N[+1.0]STAYFR
CPTAC-2231:
GPLD1.
N[+1.0]LTTSLTESVDR		CPTAC-2232:
PLOD1.
YIHQN[+1.0]YTK		CPTAC-2233:
PON1.
HAN[+1.0]WTLTPLK
CPTAC-2234:
LTBR.
AGHFQN[+1.0]TSSPSAR		CPTAC-2235:
TLR8.
GSYPQHIN[+1.0]ISR

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

 Average intra-assay CV
(within day CV)		Average inter-assay CV
(between day CV)		Total CV
equation		n=
Fragment ion / TransitionLowMedHighLowMedHighLowMedHighLowMedHigh
y4 (1+)3210.418.338.214.822.349.818.128.8151515
y5 (1+)17.812.610.920.810.310.627.416.315.2151515
y6 (1+)31.85.914.830.613.31544.114.521.1151515
sum9.65.911.311.85.111.115.27.815.8151515

Additional Resources and Comments