Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

OverviewData source: UniProt

Official Gene SymbolOther Aliases
CD79AIGA, MB1
Sequence Length (AA)Molecular Weight (Da)
22625038
Protein Name
B-cell antigen receptor complex-associated protein alpha chain
Sources
UniProt
PhosphoSitePlus ®
GeneCards		Human Protein Atlas

Protein Sequence hover to view complete sequence

CPTAC-1178: View additionalSHGGIYVC[+57.0]R data
1020304050
MPGGPGVLQALPATIFLLFLLSAVYLGPGCQALWMHKVPASLMVSLGEDA
60708090100
HFQCPHNSSNNANVTWWRVLHGNYTWPPEFLGPGEDPNGTLIIQNVNKSH
110120130140150
GGIYVCRVQEGNESYQQSCGTYLRVRQPPPRPFLDMGEGTKNRIITAEGI
160170180190200
ILLFCAVVPGTLLLFRKRWQNEKLGLDAGDEYEDENLYEGLNLDDCSMYE
210220226
DISRGLQGTYQDVGSLNIGDVQLEKP

Data source: UniProt

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database EntryPhosphoSitePlus ®

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Phosphorylation Acetylation UbiquitylationOther

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Assay Details for CPTAC-1178Collapse assay details

Data source: Panorama

Official Gene Symbol
CD79A
Peptide Modified Sequence
SHGGIYVC[+57.0]R
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
106
Peptide - Site of Modification
8
Peptide Start
99
Peptide End
107
CPTAC ID
CPTAC-1178
Peptide Molecular Mass
1,047.4920
Species
Homosapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay ParametersCollapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay MultiplexingExpand assay panel

Pacific Northwest National Laboratory-DirectMRM

CPTAC-1168:
FCER1G.
AAITSYEK		CPTAC-1169:
MELK.
DLKPENLLFDEYHK		CPTAC-1170:
PEPD.
VPLALFALNR
CPTAC-1171:
PES1.
LAALSASLAR		CPTAC-1172:
PTPRC.
LFLAEFQSIPR		CPTAC-1173:
SYK.
NYLGGFALSVAHGR
CPTAC-1174:
SYK.
LIATTAHEK		CPTAC-1175:
ANKRD27.
LYDLPDEPFTR		CPTAC-1176:
ASGR2.
ADHDALLFHLK
CPTAC-1177:
C3AR1.
STHC[+57.0]PSNNVISER		CPTAC-1178:
CD79A.
SHGGIYVC[+57.0]R		CPTAC-1179:
CEACAM1.
TIIVTELSPVVAKPQIK
CPTAC-1180:
COL3A1.
GPVGPSGPPGK		CPTAC-1181:
CYBB.
LLGSALALAR		CPTAC-1182:
EVI2B.
STLLADDLEIK
CPTAC-1183:
FCGR2B.
VTFFQNGK		CPTAC-1184:
GPR20.
YLAIVRPEGSR		CPTAC-1185:
SIX6.
AIVAFHGGNYR
CPTAC-1186:
THBS2.
GTLLALEGPGLSQR		CPTAC-1187:
TOP2A.
TLAVSGLGVVGR		CPTAC-1188:
TSPAN14.
TLISDIEAVK
CPTAC-1189:
TLE1.
FTIPESLDR		CPTAC-1190:
SLC7A9.
LGSYVQNIFTAAK		CPTAC-1191:
SLC7A9.
VLSYISVR
CPTAC-1192:
PEPD.
FEVNNTILHPEIVEC[+57.0]R		CPTAC-1193:
PES1.
FLLHEPIVNK		CPTAC-1194:
TARS.
AELNPWPEYIYTR
CPTAC-1195:
TARS.
GFQEVVTPNIFNSR		CPTAC-1196:
STARD3NL.
VLILAYAVC[+57.0]R		CPTAC-1197:
STARD3NL.
LLIVQDASER
CPTAC-1198:
ANKRD27.
LNDPSVVTPFSR		CPTAC-1199:
CD48.
LQVLDPVPKPVIK		CPTAC-1200:
CDK1.
LESEEEGVPSTAIR
CPTAC-1201:
COL11A1.
GFDGLPGLPGDK		CPTAC-1202:
COL11A1.
GEVGQIGPR		CPTAC-1203:
CYBB.
IGVFLC[+57.0]GPEALAETLSK
CPTAC-1204:
DLGAP5.
DVNIPTLEGR		CPTAC-1205:
DLGAP5.
IAQPHHGVPYFR		CPTAC-1206:
ETS1.
YENDYPSVILR
CPTAC-1207:
EVI2B.
STPGFILDTTSNK		CPTAC-1208:
LYL1.
DQAAALAAGPTPPGPR		CPTAC-1209:
LYL1.
SQPAPPADPDGSPGGAARPIK
CPTAC-1210:
MCM10.
GTNLIIQETR		CPTAC-1211:
MCM10.
IGGETLLPR		CPTAC-1212:
OSGIN1.
AVDDPGLVFNQLPK
CPTAC-1213:
PIK3CD.
GELLNPTGTVR		CPTAC-1214:
SFTPA1.
NPEENEAIASFVK		CPTAC-1215:
ZNF507.
TKPDPLIHVIQK
CPTAC-1216:
ZNF592.
AAPLIVEVFNK		CPTAC-1217:
ANXA11.
TPVLFDIYEIK		CPTAC-1218:
ANXA11.
DAQELYAAGENR

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

 Average intra-assay CV
(within day CV)		Average inter-assay CV
(between day CV)		Total CV
equation		n=
Fragment ion / TransitionLowMedHighLowMedHighLowMedHighLowMedHigh
y2 (1+)24.31315.62815.416.437.120.222.6151515
y3 (1+)13.65.88.215.88.610.320.810.413.2151515
y4 (1+)58.118.819.146.120.920.574.228.128151515
sum15.76.48.719.48.210.82510.413.9151515

Selectivity

Data source: Panorama

Slope of Curve Fit for cell line
fragment_ion123456
y3 (1+)2.415.953.887.114.726.27
y2 (1+)2.274.385.196.315.396.32
y4 (1+)2.487.285.594.937.9217.47
sum2.365.324.486.35.186.68

Stability

Data source: Panorama

Fragment ion / Transitioncontrol_intra_CVactual_temp_intra_CVfrozen_intra_CVFTx1_intra_CVFTx2_intra_CV
y4 (1+)14.112.21.711.718.6
y3 (1+)117.7633.31
y2 (1+)12.110.58.719.19.9
sum94.94.920.45.1
Fragment ion / Transitionall_intra_CVall_inter_CV
y4 (1+)11.713.8
y3 (1+)11.118.3
y2 (1+)11.821.1
sum8.215.2

Endogenous

Data source: Panorama

Fragment ion / Transitionintra_CVinter_CVtotal_CV
y4 (1+)35.134.849.4
y3 (1+)3835.251.8
y2 (1+)39.138.855.1
sum37.735.952.1

Additional Resources and Comments