Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.
OverviewData source: UniProt
| Official Gene Symbol | Other Aliases |
|---|---|
| PEPD | PRD |
| Sequence Length (AA) | Molecular Weight (Da) |
|---|---|
| 493 | 54548 |
| Protein Name |
|---|
| Xaa-Pro dipeptidase |
| Sources | |
|---|---|
| UniProt PhosphoSitePlus ® GeneCards | Human Protein Atlas |
Protein Sequence hover to view complete sequence
CPTAC-1192: View additionalFEVNNTILHPEIVEC[+57.0]R data
CPTAC-1170: View additionalVPLALFALNR data
| 10 | 20 | 30 | 40 | 50 |
| MAAATGPSFW | LGNETLKVPL | ALFALNRQRL | CERLRKNPAV | QAGSIVVLQG |
| 60 | 70 | 80 | 90 | 100 |
| GEETQRYCTD | TGVLFRQESF | FHWAFGVTEP | GCYGVIDVDT | GKSTLFVPRL |
| 110 | 120 | 130 | 140 | 150 |
| PASHATWMGK | IHSKEHFKEK | YAVDDVQYVD | EIASVLTSQK | PSVLLTLRGV |
| 160 | 170 | 180 | 190 | 200 |
| NTDSGSVCRE | ASFDGISKFE | VNNTILHPEI | VECRVFKTDM | ELEVLRYTNK |
| 210 | 220 | 230 | 240 | 250 |
| ISSEAHREVM | KAVKVGMKEY | ELESLFEHYC | YSRGGMRHSS | YTCICGSGEN |
| 260 | 270 | 280 | 290 | 300 |
| SAVLHYGHAG | APNDRTIQNG | DMCLFDMGGE | YYCFASDITC | SFPANGKFTA |
| 310 | 320 | 330 | 340 | 350 |
| DQKAVYEAVL | RSSRAVMGAM | KPGVWWPDMH | RLADRIHLEE | LAHMGILSGS |
| 360 | 370 | 380 | 390 | 400 |
| VDAMVQAHLG | AVFMPHGLGH | FLGIDVHDVG | GYPEGVERID | EPGLRSLRTA |
| 410 | 420 | 430 | 440 | 450 |
| RHLQPGMVLT | VEPGIYFIDH | LLDEALADPA | RASFLNREVL | QRFRGFGGVR |
| 460 | 470 | 480 | 490 | 493 |
| IEEDVVVTDS | GIELLTCVPR | TVEEIEACMA | GCDKAFTPFS | GPK |
Data source: UniProt
Protein MapCollapse protein map
Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs
Uniprot Database EntryPhosphoSitePlus ®
Click a point on a node to view detailed assay information below
All other points link out toUniProt
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Assay Details for CPTAC-1192Collapse assay details
Data source: Panorama
- Official Gene Symbol
- PEPD
- Peptide Modified Sequence
- FEVNNTILHPEIVEC[+57.0]R
- Modification Type
- Carbamidomethyl Cysteine
- Protein - Site of Modification
- 183
- Peptide - Site of Modification
- 15
- Peptide Start
- 169
- Peptide End
- 184
- CPTAC ID
- CPTAC-1192
- Peptide Molecular Mass
- 1,968.9727
- Species
- Homosapiens (Human)
- Assay Type
- Direct MRM or SRM
- Matrix
- Ovarian cancer tumor tissue lysate
- Submitting Laboratory
- Pacific Northwest National Laboratory
- Submitting Lab PI
- Tao Liu
Assay ParametersCollapse assay parameters
Data source: Panorama
- Instrument
- TSQ Vantage
- Internal Standard
- peptide
- Peptide Standard Purity
- >95%
- Peptide Standard Label Type
- 13C and 15N at C-terminus R
- LC
- Waters nanoACQUITY (Part Number 176016000)
- Column Packing
- Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
- Column Dimensions
- 100 um x 100 mm
- Flow Rate
- 0.5 uL/min
Assay MultiplexingExpand assay panel
Pacific Northwest National Laboratory-DirectMRM
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| Average intra-assay CV (within day CV) | Average inter-assay CV (between day CV) | Total CV | n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y5 (1+) | 38.3 | 33.2 | 27.9 | 44.9 | 22.1 | 30.6 | 59 | 39.9 | 41.4 | 15 | 15 | 15 |
| y7 (1+) | 28.3 | 12.6 | 12.1 | 26.1 | 13.8 | 20.2 | 38.5 | 18.7 | 23.5 | 15 | 15 | 15 |
| y8 (1+) | 51.8 | 19.6 | 23.6 | 77.5 | 24.8 | 23.6 | 93.2 | 31.6 | 33.4 | 15 | 15 | 15 |
| sum | 16.2 | 10.1 | 13 | 14.6 | 12.8 | 18.5 | 21.8 | 16.3 | 22.6 | 15 | 15 | 15 |
Selectivity
Data source: Panorama
| Slope of Curve Fit for cell line | ||||||
|---|---|---|---|---|---|---|
| fragment_ion | 1 | 2 | 3 | 4 | 5 | 6 |
| y7 (1+) | 0.64 | 0.56 | 1.42 | 1.37 | 1.19 | 1.72 |
| y8 (1+) | 0.46 | 0.52 | 2.88 | 0.87 | 1.42 | 1.33 |
| y5 (1+) | 0.41 | 0.75 | 2.19 | 1.12 | 1.42 | 2.54 |
| sum | 0.58 | 0.56 | 1.46 | 1.19 | 1.23 | 1.64 |
Stability
Data source: Panorama
| Fragment ion / Transition | control_intra_CV | actual_temp_intra_CV | frozen_intra_CV | FTx1_intra_CV | FTx2_intra_CV |
|---|---|---|---|---|---|
| Fragment ion / Transition | all_intra_CV | all_inter_CV |
|---|---|---|
Endogenous
Data source: Panorama
| Fragment ion / Transition | intra_CV | inter_CV | total_CV |
|---|---|---|---|
| y5 (1+) | 35.6 | 83.1 | 90.4 |
| y7 (1+) | 44.9 | 93.2 | 103.5 |
| y8 (1+) | 40.7 | 90.6 | 99.3 |
| sum | 37.6 | 87.8 | 95.5 |
Additional Resources and Comments
Comments
Assay Details for CPTAC-1170Collapse assay details
Data source: Panorama
- Official Gene Symbol
- PEPD
- Peptide Sequence
- VPLALFALNR
- Modification Type
- unmodified
- Protein - Site of Modification
- N/A
- Peptide - Site of Modification
- N/A
- Peptide Start
- 18
- Peptide End
- 27
- CPTAC ID
- CPTAC-1170
- Peptide Molecular Mass
- 1,112.6706
- Species
- Homosapiens (Human)
- Assay Type
- Direct MRM or SRM
- Matrix
- Ovarian cancer tumor tissue lysate
- Submitting Laboratory
- Pacific Northwest National Laboratory
- Submitting Lab PI
- Tao Liu
Assay ParametersCollapse assay parameters
Data source: Panorama
- Instrument
- TSQ Vantage
- Internal Standard
- peptide
- Peptide Standard Purity
- >95%
- Peptide Standard Label Type
- 13C and 15N at C-terminus R
- LC
- Waters nanoACQUITY (Part Number 176016000)
- Column Packing
- Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
- Column Dimensions
- 100 um x 100 mm
- Flow Rate
- 0.5 uL/min
Assay MultiplexingExpand assay panel
Pacific Northwest National Laboratory-DirectMRM
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| Average intra-assay CV (within day CV) | Average inter-assay CV (between day CV) | Total CV | n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y5 (1+) | 7.7 | 7.1 | 5.7 | 10.3 | 10.8 | 6.6 | 12.9 | 12.9 | 8.7 | 15 | 15 | 15 |
| y6 (1+) | 13.7 | 6.4 | 6.8 | 14.3 | 8.5 | 8.1 | 19.8 | 10.6 | 10.6 | 15 | 15 | 15 |
| y7 (1+) | 10.1 | 4.2 | 4.1 | 10.1 | 7.9 | 5.7 | 14.3 | 8.9 | 7 | 15 | 15 | 15 |
| sum | 6.5 | 4.7 | 3.2 | 7.5 | 8.2 | 5.1 | 9.9 | 9.5 | 6 | 15 | 15 | 15 |
Selectivity
Data source: Panorama
| Slope of Curve Fit for cell line | ||||||
|---|---|---|---|---|---|---|
| fragment_ion | 1 | 2 | 3 | 4 | 5 | 6 |
| y6 (1+) | 3.55 | 3.72 | 7.02 | 5.81 | 5.24 | 6.61 |
| y7 (1+) | 3.33 | 3.52 | 7.17 | 5.71 | 5.54 | 6.94 |
| y5 (1+) | 3.23 | 3.56 | 7.49 | 5.36 | 5.2 | 6.49 |
| sum | 3.35 | 3.58 | 7.22 | 5.62 | 5.35 | 6.69 |
Stability
Data source: Panorama
| Fragment ion / Transition | control_intra_CV | actual_temp_intra_CV | frozen_intra_CV | FTx1_intra_CV | FTx2_intra_CV |
|---|---|---|---|---|---|
| y5 (1+) | 5.1 | 4.1 | 5 | 3.1 | 5.8 |
| y6 (1+) | 6.4 | 3.5 | 1.2 | 5.1 | 4.9 |
| y7 (1+) | 5.1 | 3.9 | 0.6 | 1.4 | 4.1 |
| sum | 2.7 | 2.4 | 1.4 | 0.9 | 1.5 |
| Fragment ion / Transition | all_intra_CV | all_inter_CV |
|---|---|---|
| y5 (1+) | 4.5 | 4 |
| y6 (1+) | 4.1 | 4.1 |
| y7 (1+) | 3.2 | 5 |
| sum | 1.9 | 2.3 |
Endogenous
Data source: Panorama
| Fragment ion / Transition | intra_CV | inter_CV | total_CV |
|---|---|---|---|
| y7 (1+) | 38.7 | 44.5 | 59 |
| y6 (1+) | 40.3 | 45.9 | 61.1 |
| y5 (1+) | 38.6 | 43.7 | 58.3 |
| sum | 39 | 44.6 | 59.2 |