Precise genome modification in the crop species Zea mays using zinc-finger nucleases

@article{Shukla2009PreciseGM,  title={Precise genome modification in the crop species Zea mays using zinc-finger nucleases},  author={Vipula K. Shukla and Yannick Doyon and Jeffrey C. Miller and Russell Dekelver and Erica A. Moehle and Sarah E. Worden and Jon C. Mitchell and Nicole L. Arnold and Sunita M. Gopalan and Xiangdong Meng and Vivian M. Choi and Jeremy M. Rock and Ying-Ying Wu and George E. Katibah and Zhifang Gao and David G Mccaskill and Matthew A. Simpson and Beth Blakeslee and Scott A. Greenwalt and Holly Butler and Sarah Hinkley and Lei Zhang and Edward J. Rebar and Philip D. Gregory and Fyodor D. Urnov},  journal={Nature},  year={2009},  volume={459},  pages={437-441},  url={https://api.semanticscholar.org/CorpusID:4323298}}
ZFNs can be used in any plant species amenable to DNA delivery and are established as a new strategy for plant genetic manipulation in basic science and agricultural applications.

907 Citations

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49 References

Targeted mutagenesis using zinc-finger nucleases in Arabidopsis.

Data indicate that ZFNs can form the basis of a highly efficient method for targeted mutagenesis of plant genes and demonstrate efficient transmission of the ZFN-induced mutations.

Targeted transgene integration in plant cells using designed zinc finger nucleases

It is reported that designed zinc finger nucleases (ZFNs) can drive site-directed DNA integration into transgenic and native gene loci and point toward a novel approach for targeted gene addition, replacement and trait stacking in plants.

Modification of Endogenous Natural Genes by Gene Targeting in Rice and Other Higher Plants

The current status of targeting of an endogenous natural gene in rice and other higher plants is addressed and possible models for Agrobacterium- mediated gene targeting by homologous recombination using a strong positive–negative selection are discussed.

An improved zinc-finger nuclease architecture for highly specific genome editing

Using structure-based design, two variant ZFNs are engineer that modify a native endogenous locus as efficiently as the parental architecture, but with a >40-fold reduction in homodimer function and much lower levels of genome-wide cleavage.

Heritable Targeted Gene Disruption in Zebrafish Using Designed Zinc Finger Nucleases

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It is shown that zinc-finger nucleases designed against an X-linked severe combined immune deficiency mutation in the IL2Rγ gene yielded more than 18% gene-modified human cells without selection, raising the possibility of strategies based on zinc- finger nucleases for the treatment of disease.

Gene replacement by homologous recombination in plants

After the elucidation of the sequence of the yeast genome a major effort was started to elucidate the biological function of all open reading frames of this organisms by targeted gene replacement via homologous recombination, which might finally result on the set up of a general applicable technique in flowering plants.

Physical and Genetic Structure of the Maize Genome Reflects Its Complex Evolutionary History

Reconstructing the paleoethnobotany of the maize genome indicates that the progenitors of modern maize contained ten chromosomes, and a sequence-ready fingerprinted contig-based physical map is constructed to better understand maize genome organization and to build a framework for genome sequencing.

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