DOI:10.1126/science.1178811 - Corpus ID: 206522347
Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors
@article{Boch2009BreakingTC, title={Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors}, author={Jens Boch and Heidi Scholze and Sebastian Schornack and Angelika Landgraf and Simone Hahn and Sabine Kay and Thomas Lahaye and Anja Nickstadt and Ulla Bonas}, journal={Science}, year={2009}, volume={326}, pages={1509 - 1512}, url={https://api.semanticscholar.org/CorpusID:206522347}}- J. BochH. ScholzeU. Bonas
- Published inScience11 December 2009
- Biology, Environmental Science
The functionality of a distinct type of DNA binding domain is described and allows the design ofDNA binding domains for biotechnology.
2,593 Citations
2,593 Citations
TAL effector-DNA specificity
- H. ScholzeJ. Boch
- 2010
Biology, Environmental Science
The predicted DNA specificities of TAL effectors were predicted and artificial TAL proteins with novel DNA recognition specificities were generated, enabling design of proteins with potentially any given DNA recognition specificity enabling many uses for biotechnology.
Structural Basis for Sequence-Specific Recognition of DNA by TAL Effectors
- D. DengChuangye YanN. Yan
- 2012
Biology
Wrapped DNA TAL effectors are proteins that bacterial pathogens inject into plant cells that bind to host DNA to activate expression of plant genes. The DNA-binding domain of TAL proteins is composed…
TAL effectors from Xanthomonas: design of a programmable DNA-binding specificity
- H. ScholzeJana StreubelJ. BochA. BertacciniS. Maini
- 2011
Biology, Engineering
A method called "Golden TAL Technology" is developed that allows a flexible assembly of TAL proteins with a designed order of repeats, which allows a simple reprogramming of DNA-binding specificity.
TAL Effectors Specificity Stems from Negative Discrimination
- B. WickyMarco StentaM. dal Peraro
- 2013
Biology, Environmental Science
The explanatory paradigm presented herein provides a better comprehension of this elegant architecture and is hoped will allow for improved designs of TAL-derived biotechnological tools.
Predicting promoters targeted by TAL effectors in plant genomes: from dream to reality
- L. NoëlNicolás DenancéB. Szurek
- 2013
Biology, Environmental Science
An overview of the current tools and strategies that may be applied for finding targets of TAL effectors is given and several perspectives offered by these new tools are highlighted.
TAL effectors are remote controls for gene activation.
- H. ScholzeJ. Boch
- 2011
Biology, Environmental Science
[Advances in transcription activator-like effectors--a review].
This work reviewed the development of this technology in multi-level and multi species, and its advantages and disadvantages compared with ZFNs and CRISPR/Cas technology, and addressed its special advantages in industrial microbe breeding, vector construction, targeting precision, high efficiency of editing and biological safety.
The Crystal Structure of TAL Effector PthXo1 Bound to Its DNA Target
- A. MakP. BradleyR. A. CernadasA. BogdanoveB. Stoddard
- 2012
Biology, Chemistry
The crystal structure of PthXo1 bound to its DNA target was determined by high-throughput computational structure prediction and validated by heavy-atom derivatization, and illustrates the basis of TAL effector–DNA recognition.
The Use and Development of TAL Effector Nucleases
- A. JuilleratP. DuchateauT. CathomenC. Mussolino
- 2016
Biology, Engineering
The discovery of a novel DNA binding domain derived from transcription activator-like effectors of the plant pathogen genus Xanthomonas set the stage for the generation of various designer proteins by fusing tailored TALE-based DNA binding domains with either endonucleases, transcriptional modulators or chromatin remodeling domains with the final purpose to modify the genome, the transcriptome or the epigenome.
[Molecular recognition code between pathogenic bacterial TAL-effectors and host target genes: a review].
- Yanqiang LiChunlian WangKaijun Zhao
- 2011
Biology
Sheng wu gong cheng xue bao = Chinese journal of…
The findings and functions of TAL effectors, the binding specificity and recognition code between TAL-effectors and host target genes, and the possible applications and future prospects of the molecular recognition code have been discussed.
...
29 References
A Bacterial Effector Acts as a Plant Transcription Factor and Induces a Cell Size Regulator
It is shown that AvrBs3 induces the expression of a master regulator of cell size, upa20, which encodes a transcription factor containing a basic helix-loop-helix domain that provokes developmental reprogramming of host cells by mimicking eukaryotic transcription factors.
Detailed analysis of the DNA recognition motifs of the Xanthomonas type III effectors AvrBs3 and AvrBs3Deltarep16.
- S. KayS. HahnE. MaroisRobert WieduwildU. Bonas
- 2009
Biology, Environmental Science
11 new UPA genes from bell pepper that are induced by AvrBs3 early after infection with Xcv are described and it is demonstrated that the UPA box corresponds to the center of the AvrBS3-protected DNA region.
Gene-for-gene-mediated recognition of nuclear-targeted AvrBs3-like bacterial effector proteins.
- S. SchornackAnnett MeyerP. RömerTina JordanT. Lahaye
- 2006
Biology, Environmental Science
Recognition of the Bacterial Avirulence Protein AvrBs3 Occurs inside the Host Plant Cell
- G. V. D. AckervekenE. MaroisU. Bonas
- 1996
Biology, Environmental Science
Race-specificity of plant resistance to bacterial spot disease determined by repetitive motifs in a bacterial avirulence protein
- K. HerbersJ. Conrads-StrauchU. Bonas
- 1992
Biology, Environmental Science
It is reported here that the repetitive region of the avrBs3 gene determines race-specificity and that deletions of repeat units generate new avirulence specificities and unmask undiscovered resistance genes in pepper and tomato.
High frequency modification of plant genes using engineered zinc finger nucleases
- Jeffrey A. TownsendDavid A. WrightD. Voytas
- 2009
Engineering, Biology
High-frequency ZFN-stimulated gene targeting at endogenous plant genes, namely the tobacco acetolactate synthase genes (ALS SuRA and SuRB), for which specific mutations are known to confer resistance to imidazolinone and sulphonylurea herbicides are demonstrated.
The xanthomonas type III effector protein AvrBs3 modulates plant gene expression and induces cell hypertrophy in the susceptible host.
- E. MaroisG. van den AckervekenU. Bonas
- 2002
Biology, Environmental Science
It is reported that infection of susceptible pepper and tomato plants leads to an AvrBs3-dependent hypertrophy of the mesophyll tissue, and that xanthomonads possess type III effectors that steer host gene expression.
R gene expression induced by a type-III effector triggers disease resistance in rice
Xa 27 specificity towards incompatible pathogens involves the differential expression of the R gene in the presence of the AvrXa27 effector.
Two type III effector genes of Xanthomonas oryzae pv. oryzae control the induction of the host genes OsTFIIAγ1 and OsTFX1 during bacterial blight of rice
Evidence supports the hypothesis that expression of the associated host genes contributes to host susceptibility to disease and modulates the expression of multiple host genes using multiple TAL effectors from a single strain.
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