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NSP3 (rotavirus)

From Wikipedia, the free encyclopedia
Protein family
NSP3 (rotavirus)
Identifiers
SymbolRota_NSP3
PfamPF01665
InterProIPR002873
CATH1lj2A00
SCOP2d1lj2a_ /SCOPe /SUPFAM
Available protein structures:
Pfam  structures /ECOD  
PDBRCSB PDB;PDBe;PDBj
PDBsumstructure summary
Cellular vs Rotavirus Translation

Rotavirus proteinNSP3 (NS34) is bound to the 3' end consensus sequence of viralmRNAs in infected cells.[1]

Fournucleotides are the minimal requirement for RNA recognition by rotavirusnonstructural protein NSP3: using shortoligoribonucleotides, it was established that the minimalRNA sequence required for binding of NSP3A isGACC.[2]

RotavirusRNA-binding protein NSP3 interacts witheIF4GI and evicts thepoly(A)-binding protein fromeIF4F. And NSP3A, by taking the place ofPABP on eIF4GI, is responsible for the shut-off of cellular protein synthesis.[3]

Expression of NSP3 in mammalian cells allows the efficienttranslation of virus-like mRNA: NSP3 forms a link between viral mRNA and the cellular translation machinery and hence is a functional analogue of cellular poly(A)-binding protein.[4]

Site-directed mutagenesis and isothermal titration calorimetry documented that NSP3 and PABP use analogous eIF4G recognition strategies, despite marked differences in tertiary structure.[5]

Using the yeast two-hybrid assay,RoXan a novel cellular protein was found to bind NSP3. The interaction between NSP3 and RoXaN does not impair the interaction between NSP3 and eIF4GI, and a ternary complex made of NSP3, RoXaN, and eIF4G I can be detected in rotavirus-infected cells, implicating RoXaN in translation regulation.[6]

References

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  1. ^Poncet D, Aponte C, Cohen J (June 1993)."Rotavirus protein NSP3 (NS34) is bound to the 3' end consensus sequence of viral mRNAs in infected cells"(Free full text).Journal of Virology.67 (6):3159–65.PMC 237654.PMID 8388495.
  2. ^Poncet D, Laurent S, Cohen J (September 1994)."Four nucleotides are the minimal requirement for RNA recognition by rotavirus non-structural protein NSP3"(Free full text).The EMBO Journal.13 (17):4165–73.doi:10.1002/j.1460-2075.1994.tb06734.x.PMC 395339.PMID 8076612.
  3. ^Piron M, Vende P, Cohen J, Poncet D (October 1998)."Rotavirus RNA-binding protein NSP3 interacts with eIF4GI and evicts the poly(A) binding protein from eIF4F"(Free full text).The EMBO Journal.17 (19):5811–21.doi:10.1093/emboj/17.19.5811.PMC 1170909.PMID 9755181.
  4. ^Vende P, Piron M, Castagné N, Poncet D (August 2000)."Efficient translation of rotavirus mRNA requires simultaneous interaction of NSP3 with the eukaryotic translation initiation factor eIF4G and the mRNA 3' end".Journal of Virology.74 (15):7064–71.doi:10.1128/JVI.74.15.7064-7071.2000.PMC 112224.PMID 10888646.
  5. ^Groft CM, Burley SK (June 2002)."Recognition of eIF4G by rotavirus NSP3 reveals a basis for mRNA circularization".Molecular Cell.9 (6):1273–83.doi:10.1016/S1097-2765(02)00555-5.PMID 12086624.
  6. ^Vitour D, Lindenbaum P, Vende P, Becker MM, Poncet D (April 2004)."RoXaN, a novel cellular protein containing TPR, LD, and zinc finger motifs, forms a ternary complex with eukaryotic initiation factor 4G and rotavirus NSP3".Journal of Virology.78 (8):3851–62.doi:10.1128/JVI.78.8.3851-3862.2004.PMC 374268.PMID 15047801.
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