Prevalence of Aggregatibacter actinomycetemcomitans in Sudanese patients with aggressive periodontitis: a case-control study
- PMID:21332472
- DOI: 10.1111/j.1600-0765.2010.01337.x
Prevalence of Aggregatibacter actinomycetemcomitans in Sudanese patients with aggressive periodontitis: a case-control study
Abstract
Background and objective: Aggregatibacter actinomycetemcomitans is considered a possible etiological agent for aggressive periodontitis. The aim of this study was to determine the prevalence of the JP2 clone and non-JP2 genotypes of A. actinomycetemcomitans in the subgingival plaque of patients with aggressive periodontitis and controls among Sudanese high-school students.
Material and methods: In a previous study we examined a large representative sample of students attending high schools in Khartoum, Sudan. In this population, 17 patients with aggressive periodontitis and 17 controls (14-19 years of age) consented to participate in the present study. The subjects underwent a clinical periodontal examination, and subgingival dental plaque samples were collected using paper points. The presence of the A. actinomycetemcomitans JP2 clone and non-JP2 genotypes were assessed using loop-mediated isothermal amplification (LAMP) and the PCR.
Results: The JP2 clone of A. actinomycetemcomitans was not detected in the subgingival plaque of either the cases or the controls. Non-JP2 types of A. actinomycetemcomitans were detected in the subgingival plaque of 12 (70.6%) patients with aggressive periodontitis and from only one (5.9%) control subject, showing a significantly higher frequency of detection in cases than in controls (p = 0.0001). The odds ratio for the detection of A. actinomycetemcomitans in the subgingival plaque of the patients with aggressive periodontitis was 38.4 (95% confidence interval: 4.0-373.0; p = 0.002). The PCR and LAMP methods showed identical results pertaining to the identification of non-JP2 types of A. actinomycetemcomitans.
Conclusions: The JP2 clone of A. actinomycetemcomitans was not detected in the subgingival plaque of high school subjects in Sudan. The detection of non-JP2 types of A. actinomycetemcomitans may be a useful marker of increased risk for development of aggressive periodontitis in young subjects.
© 2011 John Wiley & Sons A/S.
Similar articles
- The highly leukotoxic JP2 clone of Aggregatibacter actinomycetemcomitans in localized and generalized forms of aggressive periodontitis.Ennibi OK, Benrachadi L, Bouziane A, Haubek D, Poulsen K.Ennibi OK, et al.Acta Odontol Scand. 2012 Jul;70(4):318-22. doi: 10.3109/00016357.2011.642002. Epub 2012 Jan 18.Acta Odontol Scand. 2012.PMID:22251014
- Microbiological and immunological characteristics of young Moroccan patients with aggressive periodontitis with and without detectable Aggregatibacter actinomycetemcomitans JP2 infection.Rylev M, Bek-Thomsen M, Reinholdt J, Ennibi OK, Kilian M.Rylev M, et al.Mol Oral Microbiol. 2011 Feb;26(1):35-51. doi: 10.1111/j.2041-1014.2010.00593.x. Epub 2010 Nov 18.Mol Oral Microbiol. 2011.PMID:21214871
- Stability of the JP2 clone of Aggregatibacter actinomycetemcomitans.Haubek D, Ennibi OK, Vaeth M, Poulsen S, Poulsen K.Haubek D, et al.J Dent Res. 2009 Sep;88(9):856-60. doi: 10.1177/0022034509342190.J Dent Res. 2009.PMID:19767585
- Aggregatibacter (Actinobacillus) actinomycetemcomitans: a triple A* periodontopathogen?Henderson B, Ward JM, Ready D.Henderson B, et al.Periodontol 2000. 2010 Oct;54(1):78-105. doi: 10.1111/j.1600-0757.2009.00331.x.Periodontol 2000. 2010.PMID:20712635Review.No abstract available.
- Aggregatibacter actinomycetemcomitans leukotoxin: from threat to therapy.Kachlany SC.Kachlany SC.J Dent Res. 2010 Jun;89(6):561-70. doi: 10.1177/0022034510363682. Epub 2010 Mar 3.J Dent Res. 2010.PMID:20200418Free PMC article.Review.
Cited by
- Leukotoxicity of Aggregatibacter actinomycetemcomitans in generalized aggressive periodontitis in Brazilians and their family members.Silveira VR, Nogueira MV, Nogueira NA, Lima V, Furlaneto FA, Rego RO.Silveira VR, et al.J Appl Oral Sci. 2013 Sep-Oct;21(5):430-6. doi: 10.1590/1679-775720130252.J Appl Oral Sci. 2013.PMID:24212989Free PMC article.
- Subgingival microbial profiles of Sudanese patients with aggressive periodontitis.Elabdeen HR, Mustafa M, Hasturk H, Klepac-Ceraj V, Ali RW, Paster BJ, Van Dyke T, Bolstad AI.Elabdeen HR, et al.J Periodontal Res. 2015 Oct;50(5):674-82. doi: 10.1111/jre.12250. Epub 2014 Dec 9.J Periodontal Res. 2015.PMID:25487558Free PMC article.
- Prevalence ofAggregatibacter actinomycetemcomitans and Periodontal Findings among 14 to 15-Year Old Danish Adolescents: A Descriptive Cross-Sectional Study.Jensen AB, Isidor F, Lund M, Væth M, Johansson A, Lauritsen NN, Haubek D.Jensen AB, et al.Pathogens. 2020 Dec 16;9(12):1054. doi: 10.3390/pathogens9121054.Pathogens. 2020.PMID:33339288Free PMC article.
- Periopathogenic bacteria in dental plaque of Congolese patients with periodontitis: A pilot study.Kalala-Kazadi E, Sekele-Issouradi JP, Bolenge-Ileboso J, Lasserre JF, Mantshumba-Milolo A, Ntumba-Mulumba H, Brecx MC.Kalala-Kazadi E, et al.J Clin Exp Dent. 2018 Mar 1;10(3):e232-e236. doi: 10.4317/jced.54613. eCollection 2018 Mar.J Clin Exp Dent. 2018.PMID:29721223Free PMC article.
- Clinical and Microbiological Profiles of Aggressive and Chronic Periodontitis in Congolese Patients: A Cross-sectional Study.Kalala-Kazadi E, Toma S, Lasserre JF, Nyimi-Bushabu F, Ntumba-Mulumba H, Brecx MC.Kalala-Kazadi E, et al.J Int Soc Prev Community Dent. 2020 Aug 6;10(4):491-497. doi: 10.4103/jispcd.JISPCD_501_19. eCollection 2020 Jul-Aug.J Int Soc Prev Community Dent. 2020.PMID:33042892Free PMC article.
Publication types
MeSH terms
Substances
Related information
LinkOut - more resources
Full Text Sources