5'-Triphosphate RNA is the ligand for RIG-I
- PMID:17038590
- DOI: 10.1126/science.1132505
5'-Triphosphate RNA is the ligand for RIG-I
Abstract
The structural basis for the distinction of viral RNA from abundant self RNA in the cytoplasm of virally infected cells is largely unknown. We demonstrated that the 5'-triphosphate end of RNA generated by viral polymerases is responsible for retinoic acid-inducible protein I (RIG-I)-mediated detection of RNA molecules. Detection of 5'-triphosphate RNA is abrogated by capping of the 5'-triphosphate end or by nucleoside modification of RNA, both occurring during posttranscriptional RNA processing in eukaryotes. Genomic RNA prepared from a negative-strand RNA virus and RNA prepared from virus-infected cells (but not from noninfected cells) triggered a potent interferon-alpha response in a phosphatase-sensitive manner. 5'-triphosphate RNA directly binds to RIG-I. Thus, uncapped 5'-triphosphate RNA (now termed 3pRNA) present in viruses known to be recognized by RIG-I, but absent in viruses known to be detected by MDA-5 such as the picornaviruses, serves as the molecular signature for the detection of viral infection by RIG-I.
Comment in
- Virology. Sensing viral RNA amid your own.Fujita T.Fujita T.Science. 2006 Nov 10;314(5801):935-6. doi: 10.1126/science.1135756.Science. 2006.PMID:17095686No abstract available.
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