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An R package for weighted region comethylation network analysis.

Comethyl builds upon the WGCNA package to identify and interpret modules ofcomethylated regions from whole-genome bisulfite sequencing data. Regions aredefined from clusters of CpG sites or from genomic annotations, and then percentmethylation values are used to identify comethylation modules.

Interesting modules are identified and explored by comparing with sample traits andexamining functional enrichments. Results are then visualized with high-quality,editable plots from ggplot2.

You can install Comethyl from this repository and load it into your R session with the code below.

install.packages(c("BiocManager", "remotes"))BiocManager::install("cemordaunt/comethyl")library(comethyl)

Complete documentation for comethyl is available athttps://cemordaunt.github.io/comethyl/.

• Introduction to Comethyl

• Function reference

• CpG cluster analysis vignette

• Gene body analysis vignette

• Module preservation vignette

If you use Comethyl in your work, please cite ourBriefings in Bioinformaticspublication:

Mordaunt CE, Mouat JS, Schmidt RJ, and LaSalle JM. (2022) Comethyl: a network-based methylome approach to investigate the multivariate nature of health and disease.Briefings in Bioinformatics bbab554.

Many thanks to Julia Mouat for creating the vignettes, and to both Ben Laufer andJanine LaSalle for very helpful discussions.