 | |
| Names | |
|---|---|
| IUPAC name N-[1,3-Dihydroxy-2-(hydroxymethyl)propan-2-yl]glycine | |
| Systematic IUPAC name {[1,3-Dihydroxy-2-(hydroxymethyl)propan-2-yl]amino}acetic acid | |
| Other names Tricine N-(Tri(hydroxymethyl)methyl)glycine | |
| Identifiers | |
3D model (JSmol) | |
| 1937804 | |
| ChEBI | |
| ChemSpider |
|
| ECHA InfoCard | 100.024.721 |
| EC Number |
|
| 3688 | |
| MeSH | tricine |
| UNII | |
| |
| |
| Properties | |
| C6H13NO5 | |
| Molar mass | 179.172 g·mol−1 |
| Appearance | White crystals |
| 89.6 g L−1 (at 20 °C) | |
| UV-vis (λmax) | 260 nm |
| Absorbance | 0.03 |
| Related compounds | |
Related compounds | Milacemide |
Except where otherwise noted, data are given for materials in theirstandard state (at 25 °C [77 °F], 100 kPa). | |
Tricine is anorganic compound that is used inbuffer solutions. The name tricine comes fromtris andglycine, from which it was derived.[1] It is a white crystalline powder that is moderately soluble in water. It is azwitterionicamino acid that has a pKa1 value of 2.3 at 25 °C, while its pKa2 at 20 °C is 8.15. Its useful buffering range of pH is 7.4-8.8. Along withbicine, it is one ofGood's buffering agents. Good first prepared tricine to buffer chloroplast reactions.
Tricine is a commonly usedelectrophoresis buffer and is also used in resuspension of cell pellets. It has a higher negative (more negative) charge thanglycine allowing it to migrate faster. In addition its high ionic strength causes moreion movement and lessprotein movement. This allows for lowmolecular weight proteins to be separated in lower percentacrylamide gels. Tricine has been documented in the separation of proteins in the range of 1 to 100kDa by electrophoresis.[2] The tricine buffer at 25 mmol/L was found to be the most effective buffer among the ten tested forATP assays usingfirefly luciferase.[3] Tricine has also been found to be an effective scavenger of hydroxyl radicals in a study of radiation-induced membrane damage.[4]
