TheTIC and TOC complexes aretranslocons located in thechloroplast of aeukaryotic cell, that is,protein complexes that facilitate the transfer of proteins in and out through the chloroplast's membrane. It mainly transports proteins made in the cytoplasm into the chloroplast.[1] TheTIC complex(translocon on theinnerchloroplast membrane) is located in the inner envelope of the chloroplast. TheTOC complex(translocon on theouterchloroplast membrane) is located in the outer envelope of the chloroplast.
This protein complex is functionally similar to theTOM/TIM Complex located on the outer and inner membranes of the mitochondria, in the sense that it too transports proteins across multiple membranes and into the lumen of an organelle. Both complexes (TOC/TIC) are GTPases, that is, they must both hydrolyze GTP in order to power the translocation. The chloroplast also harnesses the power of an electrochemical gradient using protons. The gradient is only used to power transport across the thylakoid membrane, however, while the gradient in the mitochondria is used to power transport across its inner membrane.[3]
Furthermore, due to the thylakoid membrane located inside of the chloroplast, a second transit peptide sequence must be located on the imported protein. In the cytosol, a transit peptide that signals for transit of the protein to the chloroplast is exposed. This initiates transport and translocation through the TIC/TOC complexes into the stroma of the chloroplast. It is there that a signal peptidase cleaves the stromal transit peptide, only to reveal a second transit peptide sequence underneath; this time directing to the thylakoid membrane.[4] There are at least three ways for the protein to go through the thylakoid membrane: through a ATP-hydrolyzingType II secretion system, through aSecY translocon, or through theTat/VSP pathway.[5]
| AIG1-type guanine nucleotide-binding (G) domain | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| Symbol | G_AIG1 | ||||||||
| Pfam | PF04548 | ||||||||
| InterPro | IPR006703 | ||||||||
| TCDB | 3.A.9 | ||||||||
| Membranome | 176 | ||||||||
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