Sodium channels areintegral membrane proteins that formion channels, conductingsodium ions (Na⁺) through acell'smembrane.^[1][2] They belong to thesuperfamily of cation channels.

They are classified into 2 types:

Inexcitable cells such asneurons,myocytes, and certain types ofglia, sodium channels are responsible for therising phase ofaction potentials. These channels go through three different states called resting, active and inactive states. Even though the resting and inactive states would not allow the ions to flow through the channels the difference exists with respect to their structural conformation.

Sodium channels are highly selective for the transport of ions across cell membranes. The high selectivity with respect to the sodium ion is achieved in many different ways. All involve encapsulation of the sodium ion in a cavity of specific size within a larger molecule.^[3]

Sodium channels consist of largealpha subunits that associate with accessory proteins, such asbeta subunits. An alpha subunit forms the core of the channel and is functional on its own. When the alpha subunit protein is expressed by a cell, it is able to form a pore in the cell membrane that conducts Na⁺ in a voltage-dependent way, even if beta subunits or other known modulating proteins are not expressed. When accessory proteins assemble with α subunits, the resulting complex can display altered voltage dependence and cellular localization.

The alpha subunit consists of four repeat domains, labelled I through IV, each containing six membrane-spanning segments, labelled S1 through S6. The highlyconserved S4 segment acts as the channel's voltage sensor. The voltage sensitivity of this channel is due to positive amino acids located at every third position.^[5] When stimulated by a change intransmembrane voltage, this segment moves toward the extracellular side of the cell membrane, allowing the channel to become permeable to ions. The ions are conducted through the central pore cavity, which consists of two main regions. The more external (i.e., more extracellular) portion of the pore is formed by the "P-loops" (the region between S5 and S6) of the four domains. This region is the most narrow part of the pore and is responsible for its ion selectivity. The inner portion (i.e., more cytoplasmic) of the pore is the pore gate and is formed by the combined S5 and S6 segments of the four domains. The pore domain also features lateral tunnels or fenestrations that run perpendicular to the pore axis. These fenestrations that connect the central cavity to the membrane are proposed to be important for drug accessibility.^[6][7][8]

In mammalian sodium channels, the region linking domains III and IV is also important for channel function. This DIII-IV linker is responsible for wedging the pore gate shut after channel opening, inactivating it.^[9]

Voltage-gated Na⁺ channels have three main conformational states: closed, open and inactivated. Forward/back transitions between these states are correspondingly referred to as activation/deactivation (between open and closed, respectively), inactivation/reactivation (between inactivated and open, respectively), and recovery from inactivation/closed-state inactivation (between inactivated and closed, respectively). Closed and inactivated states are ion impermeable.

Before an action potential occurs, the axonal membrane is at its normalresting potential, about −70 mV in most human neurons, and Na⁺ channels are in their deactivated state, blocked on the extracellular side by theiractivation gates. In response to an increase of the membrane potential to about −55 mV (in this case, caused by an action potential), the activation gates open, allowing positively charged Na⁺ ions to flow into the neuron through the channels, and causing the voltage across the neuronal membrane to increase to +30 mV in human neurons. Because the voltage across the membrane is initially negative, as its voltage increasesto andpast zero (from −70 mV at rest to a maximum of +30 mV), it is said to depolarize. This increase in voltage constitutes the rising phase of an action potential.

At the peak of the action potential, when enough Na⁺ has entered the neuron and the membrane's potential has become high enough, the Na⁺ channels inactivate themselves by closing theirinactivation gates. The inactivation gate can be thought of as a "plug" tethered to domains III and IV of the channel's intracellular alpha subunit. Closure of the inactivation gate causes Na⁺ flow through the channel to stop, which in turn causes the membrane potential to stop rising. The closing of the inactivation gate creates a refractory period within each individual Na⁺ channel. This refractory period eliminates the possibility of an action potential moving in the opposite direction back towards the soma. With its inactivation gate closed, the channel is said to be inactivated. With the Na⁺ channel no longer contributing to the membrane potential, the potential decreases back to its resting potential as the neuron repolarizes and subsequently hyperpolarizes itself, and this constitutes the falling phase of an action potential. The refractory period of each channel is therefore vital in propagating the action potential unidirectionally down an axon for proper communication between neurons.

When the membrane's voltage becomes low enough, the inactivation gate reopens and the activation gate closes in a process calleddeinactivation. With the activation gate closed and the inactivation gate open, the Na⁺ channel is once again in its deactivated state, and is ready to participate in another action potential.

When any kind of ion channel does not inactivate itself, it is said to be persistently (or tonically) active. Some kinds of ion channels are naturally persistently active. However, genetic mutations that cause persistent activity in other channels can cause disease by creating excessive activity of certain kinds of neurons. Mutations that interfere with Na⁺ channel inactivation can contribute to cardiovascular diseases or epileptic seizures bywindow currents, which can cause muscle and/or nerve cells to become over-excited.

The temporal behavior of Na⁺ channels can be modeled by aMarkovian scheme or by theHodgkin–Huxley-type formalism. In the former scheme, each channel occupies a distinctstate withdifferential equations describing transitions between states; in the latter, the channels are treated as apopulation that are affected by three independent gating variables. Each of these variables can attain a value between 1 (fully permeant to ions) and 0 (fully non-permeant), the product of these variables yielding the percentage of conducting channels. The Hodgkin–Huxley model can be shown to be equivalent to a Markovian model.^{[further explanation needed]}

The pore of sodium channels contains aselectivity filter made of negatively chargedamino acid residues, which attract the positive Na⁺ ion and keep out negatively charged ions such aschloride. The cations flow into a more constricted part of the pore that is 0.3 by 0.5nm wide, which is just large enough to allow a single Na⁺ ion with a watermolecule associated to pass through. The larger K⁺ ion cannot fit through this area. Ions of different sizes also cannot interact as well with the negatively chargedglutamic acid residues that line the pore.^{[citation needed]}

Voltage-gated sodium channels normally consist of an alpha subunit that forms the ion conduction pore and one to two beta subunits that have several functions including modulation of channel gating.^[10] Expression of the alpha subunit alone is sufficient to produce a functional channel.

The family of sodium channels has 9 known members, with amino acid identity >50% in the trans-membrane segments and extracellular loop regions. A standardized nomenclature for sodium channels is currently used and is maintained by theIUPHAR.^[11]

The proteins of these channels are named Na_v1.1 through Na_v1.9. The gene names are referred to as SCN1A through SCN5A, then SCN8A through SCN11A.^[11] The "tenth member", Na_x, does not act in a voltage-gated way. It has a loosely similar overall structure. Not much is known about its real function, other than that it also associates with beta subunits.^[12]

The probable evolutionary relationship between these channels, based on the similarity of their amino acid sequences, is shown in figure 1. The individual sodium channels are distinguished not only by differences in their sequence but also by their kinetics and expression profiles. Some of this data is summarized in table 1, below.

Sodium channel beta subunits are type 1 transmembrane glycoproteins with an extracellular N-terminus and a cytoplasmic C-terminus. As members of the Ig superfamily, beta subunits contain a prototypic V-set Ig loop in their extracellular domain. They do not share any homology with their counterparts of calcium and potassium channels.^[21] Instead, they are homologous to neural cell adhesion molecules (CAMs) and the large family of L1 CAMs. There are four distinct betas named in order of discovery: SCN1B, SCN2B, SCN3B, SCN4B (table 2). Beta 1 and beta 3 interact with the alpha subunit non-covalently, whereas beta 2 and beta 4 associate with alpha via disulfide bond.^[22] Sodium channels are more likely to stay open at the subthreshold membrane potential when interacting with beta toxins, which in turn induces an immediate sensation of pain.^[23]

In addition to regulating channel gating, sodium channel beta subunits also modulate channel expression and form links to theintracellularcytoskeleton viaankyrin andspectrin.^[10][24][25]Voltage-gated sodium channels also assemble with a variety of other proteins, such as FHF proteins (Fibroblast growth factor Homologous Factor), calmodulin, cytoskeleton or regulatory kinases,^{[26][10][27][28][29]} which form a complex with sodium channels, influencing its expression and/or function. Several beta subunits interact with one or moreextracellular matrix (ECM) molecules. Contactin, also known as F3 or F11, associates with beta 1 as shown via co-immunoprecipitation.^[30]Fibronectin-like (FN-like) repeats ofTenascin-C andTenascin-R bind with beta 2 in contrast to theEpidermal growth factor-like (EGF-like) repeats that repel beta2.^[31] A disintegrin and metalloproteinase (ADAM) 10 sheds beta 2'sectodomain possibly inducing neurite outgrowth.^[32] Beta 3 and beta 1 bind to neurofascin at Nodes of Ranvier in developing neurons.^[33]

Ligand-gated sodium channels are activated by binding of aligand instead of a change in membrane potential.

They are found, e.g. in theneuromuscular junction asnicotinic receptors, where the ligands areacetylcholine molecules. Most channels of this type are permeable to potassium to some degree as well as to sodium.

Voltage-gated sodium channels play an important role inaction potentials. If enough channels open when there is a change in the cell'smembrane potential, a small but significant number of Na⁺ ions will move into the cell down theirelectrochemical gradient, furtherdepolarizing the cell. Thus, the more Na⁺ channels localized in a region of a cell's membrane the faster the action potential will propagate and the moreexcitable that area of the cell will be. This is an example of apositive feedback loop. The ability of these channels to assume a closed-inactivated state causes therefractory period and is critical for the propagation of action potentials down anaxon.

Na⁺ channels both open and close more quickly thanK⁺ channels, producing an influx of positive charge (Na⁺) toward the beginning of theaction potential and an efflux (K⁺) toward the end.

Ligand-gated sodium channels, on the other hand, create the change in the membrane potential in the first place, in response to the binding of a ligand to it. Leak sodium channels additionally contribute to action potential regulation by modulating the resting potential (and in turn, the excitability) of a cell.^[35]

The following naturally produced substances persistently activate (open) sodium channels:

• Alkaloid-based toxins
  • aconitine
  • batrachotoxin
  • brevetoxin
  • ciguatoxin
  • delphinine
  • somegrayanotoxins, e.g., grayanotoxin I (other granotoxins inactive, or close, sodium channels)
  • veratridine

The following toxins modify the gating of sodium channels:

• Peptide-based toxins
  • μ-Conotoxin
  • δ-Atracotoxin^[36]
  • Scorpion venom toxins^[37]

Sodium leak channels do not show any voltage or ligand gating. Instead, they are always open or "leaking" a small background current to regulate the resting membrane potential of a neuron.^[35] In most animals, a single gene encodes the NALCN (sodium leak channel, nonselective) protein.^[38]

Despite following the same basic structure as other sodium channels, NALCN is not sensitive to voltage changes. The voltage-sensitive S4 transmembrane domain of NALCN has fewer positively charged amino acids (13 instead of a voltage gated channel's 21) possibly explaining its voltage insensitivity.^[35] NALCN is also far less selective for Na⁺ ions and is permeable to Ca²⁺ and K⁺ ions. The EEKE amino acid motif in the pore filter domain of NALCN is similar to both the EEEE motif ofvoltage-gated calcium channel and the DEKA motif of the voltage-gated sodium channel, possibly explaining its lack of selectivity.^[38]

NALCN is not blocked by many common sodium channel blockers, includingtetrodotoxin. NALCN is blocked nonspecifically by both Gd³⁺ andverapamil.^[39]Substance P andneurotensin both activateSrc family kinases through their respectiveGPCRs (independent of the coupledG-proteins) which in turn increase the permeability of NALCN through UNC80 activation.^[40]Acetylcholine can also increase NALCN activity throughM₃ muscarinic acetylcholine receptors.^[41] Higher levels of extracellular Ca²⁺ decrease the permeability of NALCN by activatingCaSR which inhibits UNC80.^[42]

NALCN complexes with the proteins UNC79, UNC80, and FAM155A.^[43][44][45] UNC79 appears to be linked to membrane stability of NALCN and linkage with UNC 80.^[44] UNC80 mediates chemical modulation of NALCN through multiple pathways.^{[35][42][41][40]} FAM155A helps protein folding in the endoplasmic reticulum, chaperones transport to the axon, and contributes to membrane stability.^[45]

Theresting membrane potential of a neuron is usually -60mV to -80mV, driven primarily by the K⁺ potential at -90mV. The depolarization from the K⁺ potential is due primarily to a small Na⁺ leak current. About 70% of this current is through NALCN.^[39] Increasing NALCN permeability lowers the resting membrane potential, bringing it closer to the trigger of an action potential (-55mV), thus increasing the excitability of a neuron.

Mutations to NALCN lead to severe disruptions to respiratory rhythm in mice^[39] and altered circadian locomotion in flies.^[46] Mutations to NALCN have also been linked to multiple severe developmental disorders^[47] and cervical dystonia.^[48] Schizophrenia and bipolar disorder are also linked to mutations to NALCN.^[49]

Changes in blood and tissue pH accompany physiological and pathophysiological conditions such as exercise, cardiac ischemia, ischemic stroke, and cocaine ingestion. These conditions are known to trigger the symptoms of electrical diseases in patients carrying sodium channel mutations. Protons cause a diverse set of changes to sodium channel gating, which generally lead to decreases in the amplitude of the transient sodium current and increases in the fraction of non-inactivating channels that pass persistent currents. These effects are shared with disease-causing mutants in neuronal, skeletal muscle, and cardiac tissue and may be compounded in mutants that impart greater proton sensitivity to sodium channels, suggesting a role of protons in triggering acute symptoms of electrical disease.^[50]

Single channel data from cardiomyocytes have shown that protons can decrease the conductance of individual sodium channels.^[51] The sodium channel selectivity filter is composed of a single residue in each of the four pore-loops of the four functional domains. These four residues are known as the DEKA motif.^[52] The permeation rate of sodium through the sodium channel is determined by a four carboxylate residues, the EEDD motif, which make up the outer charged ring.^[52] The protonation of these carboxylates is one of the main drivers of proton block in sodium channels, although there are other residues that also contribute to pH sensitivity.^[53] One such residue is C373 in thecardiac sodium channel which makes it the most pH-sensitive sodium channel among the sodium channels that have been studied to date.^[54]

As the cardiac sodium channel is the most pH-sensitive sodium channel, most of what is known is based on this channel. Reduction in extracellular pH has been shown to depolarize the voltage-dependence of activation and inactivation to more positive potentials. This indicates that during activities that decrease the blood pH, such as exercising, the probability of channels activating and inactivating is higher more positive membrane potentials, which can lead to potential adverse effects.^[55] The sodium channels expressed in skeletal muscle fibers have evolved into relatively pH-insensitive channels. This has been suggested to be a protective mechanism against potential over- or under-excitability in skeletal muscles, as blood pH levels are highly susceptible to change during movement.^[56][57] Recently, a mixed syndrome mutation that causes periodic paralysis and myotonia in the skeletal sodium channel has been shown to impart pH-sensitivity in this channel, making the gating of this channel similar to that of the cardiac subtype.^[58]

The effects of protonation have been characterized in Na_v1.1–Na_v1.5. Among these channels, Na_v1.1–Na_v1.3 and Na_v1.5 display depolarized voltage-dependence of activation, while activation in Na_v1.4 remains insensitive to acidosis. The voltage-dependence of steady-state fast inactivation is unchanged in Na_v1.1–Na_v1.4, but steady-state fast inactivation in Na_v1.5 is depolarized. Hence, among the sodium channels that have been studied so far, Na_v1.4 is the least and Na_v1.5 is the most proton-sensitive subtypes.^[59]

• Sodium+Channels at the U.S. National Library of MedicineMedical Subject Headings (MeSH)
• "Voltage-Gated Sodium Channels".IUPHAR Database of Receptors and Ion Channels. International Union of Basic and Clinical Pharmacology. Archived fromthe original on 2021-12-09. Retrieved2008-12-18.

• Electrophysiology
• Integral membrane proteins
• Sodium channels

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