| Pullulanase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
 Debranching enzyme homotetramer,Klebsiella oxytoca | |||||||||
| Identifiers | |||||||||
| EC no. | 3.2.1.41 | ||||||||
| CAS no. | 9075-68-7 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDBPDBePDBsum | ||||||||
| |||||||||
Pullulanase (EC3.2.1.41,limit dextrinase,amylopectin 6-glucanohydrolase,bacterial debranching enzyme,debranching enzyme,α-dextrin endo-1,6-α-glucosidase,R-enzyme,pullulan α-1,6-glucanohydrolase) is a specific kind ofglucanase, an amylolyticexoenzyme, that degradespullulan.[1][2][3] It is produced as an extracellular, cell surface-anchoredlipoprotein byGram-negative bacteria of the genusKlebsiella. Type I pullulanases specifically attack α-1,6 linkages, while type II pullulanases are also able tohydrolyse α-1,4 linkages. It is also produced by some otherbacteria andarchaea. Pullulanase is used as a processing aid in grain processingbiotechnology (production ofethanol and sweeteners).
Pullulanase is also known as pullulan-6-glucanohydrolase (Debranching enzyme). Its substrate, pullulan, is regarded as a chain of maltotriose units linked by α-1,6-glycosidic bonds. Pullulanase will hydrolytically cleave pullulan (α-glucan polysaccharides).
In the food industry, pullulanase works well as an ingredient. Pullulan can be applied directly to foods as a protective glaze or edible film due to its ability to form films. It can be used as a spice and flavoring agent formicro-encapsulation.[4] It is used in mayonnaise to maintain consistency and quality. It is additionally used in low-calorie food formulations as a starch replacement.
Pullulanase can be used to convert starches in grains into fermentable sugars, which yeast can use to produce alcohol duringfermentation.
