Apolyphenol antioxidant is ahypothesized type ofantioxidant studiedin vitro. Numbering over 4,000 distinctchemical structures mostly fromplants, such polyphenols have not been demonstrated to be antioxidantsin vivo.[1][2][3]
In vitro at high experimental doses, polyphenols may affect cell-to-cell signaling,receptor sensitivity, inflammatoryenzyme activity orgene regulation.[3][4] None of these hypothetical effects has been confirmed in humans by high-qualityclinical research, as of 2020[update].[1]
The main source of polyphenols is dietary, since they are found in a wide array ofphytochemical-bearing foods. For example,honey; mostlegumes; fruits such asapples,blackberries,blueberries,cantaloupe,pomegranate,cherries,cranberries,grapes,pears,plums,raspberries,aronia berries, andstrawberries (berries in general have high polyphenol content[5]) andvegetables such asbroccoli,cabbage,celery,onion andparsley are rich in polyphenols.Red wine,chocolate, black tea,white tea,green tea,olive oil and manygrains are sources.[1] Ingestion of polyphenols occurs by consuming a wide array of plant foods.[1]
The regulation theory considers a polyphenolic ability to scavengefree radicals and up-regulate certain metalchelation reactions.[1] Variousreactive oxygen species, such assinglet oxygen,peroxynitrite andhydrogen peroxide, must be continually removed from cells to maintain healthy metabolic function. Diminishing the concentrations of reactive oxygen species can have several benefits possibly associated withion transport systems and so may affectredox signaling.[1] There is no substantial evidence, however, that dietary polyphenols have an antioxidant effect in vivo.[1][6]
The “deactivation” of oxidant species by polyphenolic antioxidants (POH) is based, with regard to food systems that are deteriorated by peroxyl radicals (R•), on the donation of hydrogen, which interrupts chain reactions:
Phenoxyl radicals (PO•) generated according to this reaction may be stabilized throughresonance and/or intramolecular hydrogen bonding, as proposed forquercetin, or combine to yielddimerisation products, thus terminating the chain reaction:
Consuming dietary polyphenols have been evaluated for biological activity in vitro, but there is no evidence from high-qualityclinical research as of 2015[update] that they have effects in vivo.[1] Preliminary research has been conducted and regulatory status was reviewed in 2009 by the U.S.Food and Drug Administration (FDA), with no recommended intake values established, indicating absence of proof for nutritional value.[6] Other possible effects may result from consumption of foods rich in polyphenols, but are not yet proved scientifically in humans; accordingly, health claims on food labels are not allowed by the FDA.[6]
It is difficult to evaluate the physiological effects of specific natural phenolic antioxidants, since such a large number of individual compounds may occur even in a single food and their fate in vivo cannot be measured.[1][6][8]
Other more detailed chemical research has elucidated the difficulty of isolating individual phenolics. Because significant variation in phenolic content occurs among various brands of tea, there are possible[9] inconsistencies among epidemiological studies implying beneficial health effects of phenolic antioxidants ofgreen tea blends. TheOxygen Radical Absorbance Capacity (ORAC) test is a laboratory indicator of antioxidant potential in foods anddietary supplements. However, ORAC results cannot be confirmed to be physiologically applicable and have been designated as unreliable.[3][10]
There is debate regarding the total body absorption of dietary intake of polyphenolic compounds. While some indicate potential health effects of certain specific polyphenols, most studies demonstrate lowbioavailability and rapid excretion of polyphenols, indicating their potential roles only in small concentrations in vivo.[1][2][3][4] More research is needed to understand the interactions between a variety of these chemicals acting in concert within the human body.[1]
There is no substantial evidence that reactive oxygen species play a role in the process of skinaging.[11] The skin is exposed to variousexogenous sources ofoxidative stress, includingultraviolet radiation whose spectral components may be responsible for theextrinsic type of skin aging, sometimes termedphotoaging. Controlled long-term studies on the efficacy of low molecular weight antioxidants in the prevention or treatment ofskin aging in humans are absent.
Experiments onlinoleic acid subjected to2,2′-azobis (2-amidinopropane) dihydrochloride-induced oxidation with different combinations of phenolics show that binary mixtures can lead to either asynergetic effect or to anantagonistic effect.[12]
Antioxidant levels of purified anthocyanin extracts were much higher than expected from anthocyanin content indicatingsynergistic effect of anthocyanin mixtures.[13]