Phytosterols arephytosteroids, similar tocholesterol, that serve as structural components of biological membranes ofplants.[1] They encompass plantsterols andstanols.[1] More than 250 sterols and related compounds have been identified.[2] Free phytosterols extracted from oils are insoluble in water, relatively insoluble in oil, and soluble in alcohols.
Nomenclature of the structure of a tetracyclic damarane triterpene
They have a fused polycyclic structure and vary in carbon side chains and / or presence or absence of adouble bond (saturation).[3] They[clarification needed] are divided into 4,4-dimethyl phytosterols, 4-monomethyl phytosterols, and 4-desmethyl phytosterols based on the location of methyl groups at the carbon-4 position.[6] Stanols aresaturated sterols, having no double bonds in the sterol ring structure.
The molecule in the article lead isβ-sitosterol. The nomenclature is shown on the right.
By removing carbons 241 and 242,cholesterol is obtained.
Removing a hydrogen from carbons 22 and 23 yieldsstigmasterol (stigmasta-5,22-dien-3β-ol).
By hydrogenating the double bond between carbons 5 and 6, β-sitostanol (Stigmastanol) is obtained.
By hydrogenating the double bond between carbons 5 and 6 and removing carbon 242,campestanol is obtained.
Removing carbon 242 and hydrogens from carbons 22 and 23, and inverting the stereochemistry at C-24 yieldsbrassicasterol (ergosta-5,22-dien-3β-ol).
Further removal of hydrogens from carbons 7 and 8 from brassicasterol yieldsergosterol (ergosta-5,7,22-trien-3β-ol). Important: Ergosterol is not a plant sterol. Ergosterol is a component of fungal cell membranes, serving the same function in fungi that cholesterol serves in animal cells.
In addition:
Esterification of the hydroxyl group at carbon 3 with fatty/organic acids or carbohydrates results in plantsterol esters, i.e. oleates, ferulates and (acyl) glycosides.
The richest naturally occurring sources of phytosterols are vegetable oils and products made from them. Sterols can be present in the free form and asfatty acid esters andglycolipids. The bound form is usually hydrolyzed in the small intestines bypancreatic enzymes.[7] Some of the sterols are removed during the deodorization step ofrefining oils and fats, without, however, changing their relative composition. Sterols are therefore a useful tool in checking authenticity.
As common sources of phytosterols,vegetable oils have been developed asmargarine products highlighting phytosterol content.[3] Cereal products, vegetables, fruit and berries, which are not as rich in phytosterols, may also be significant sources of phytosterols due to their higher intakes.[8]
The intake of naturally occurring phytosterols ranges between ~200–300 mg/day depending on eating habits.[9] Specially designed vegetarian experimental diets have been produced yielding upwards of 700 mg/day.[10] The most commonly occurring phytosterols in the human diet are β-sitosterol, campesterol and stigmasterol,[3] which account for about 65%, 30% and 3% of diet contents, respectively.[11] The most common plantstanols in the human diet are sitostanol and campestanol, which combined make up about 5% of dietary phytosterol.[12]
Sterol composition in crude oils (as percentage of total sterol fraction)[13]
The European Foods Safety Authority (EFSA) concluded thatblood cholesterol can be reduced on average by 7 to 10.5% if a person consumes 1.5 to 2.4 grams of plant sterols and stanols per day, an effect usually established within 2–3 weeks. Longer-term studies extending up to 85 weeks showed that the cholesterol-lowering effect could be sustained.[14] Based on this and other efficacy data, the EFSA scientific panel provided the following health advisory: "Plant sterols have been shown to lower/reduce blood cholesterol. Blood cholesterol lowering may reduce the risk ofcoronary heart disease".[15]
TheFDA has approved the following claim for phytosterols:For plantsterol esters: (i) Foods containing at least 0.65 g per serving of plant sterol esters, eaten twice a day with meals for a daily total intake of at least 1.3 g, as part of a diet low in saturated fat and cholesterol, may reduce the risk of heart disease. A serving of [name of the food] supplies ___grams of vegetable oil sterol esters.[16]For plant stanol esters: (i) Foods containing at least 1.7 g per serving of plant stanol esters, eaten twice a day with meals for a total daily intake of at least 3.4 g, as part of a diet low in saturated fat and cholesterol, may reduce the risk of heart disease. A serving of [name of the food] supplies ___grams of plant stanol esters.[17]Reviewing clinical trials involving phytosterol supplementation, the FDA concluded that when consumed in the range of 1 to 3 grams in enriched foods, phytosterols resulted in statistically significant (5-15%) reductions in blood LDL cholesterol levels relative to placebo. The FDA also concluded that a daily dietary intake of 2 grams a day of phytosterols (expressed as non-esterified phytosterols) is required to demonstrate a relationship between phytosterol consumption and cholesterol lowering for reduced CVD risk.[18]
Health Canada reviewed the evidence of 84 randomized controlled trials published between 1994 and 2007 involving phytosterol supplementation. An average 8.8% reduction in LDL-cholesterol was observed at a mean intake of 2 grams per day.[19] Health Canada concluded that sufficient scientific evidence exists to support a relationship between phytosterol consumption and blood cholesterol lowering. Based on this evidence, Health Canada approved the following statements for qualifying foods intended forhypercholesterolemic individuals:Primary statement:"[serving size from Nutrition Facts table in metric and common household measures] of [naming the product] provides X% of the daily amount* of plant sterols shown to help reduce/lower cholesterol in adults."Two additional statements that could be used in combination or alone, adjacent to the primary statement, without any intervening printed, written or graphic material:"Plant sterols help reduce [or help lower] cholesterol." This statement when used, shall be shown in letters up to twice the size and prominence as those of the primary statement."High cholesterol is a risk factor for heart disease." This statement when used, shall be shown in letters up to the same size and prominence as those of the primary statement.
The ability of phytosterols to reducecholesterol levels was first demonstrated in humans in 1953.[20][21] From 1954 to 1982, phytosterols were subsequently marketed as a pharmaceutical under the name Cytellin as a treatment for elevated cholesterol.[22]
Unlike thestatins, where cholesterol lowering has been proven to reduce risk ofcardiovascular diseases (CVD) and overall mortality under well-defined circumstances, the evidence has been inconsistent for phytosterol-enriched foods or supplements to lower risk of CVD, with two reviews indicating no or marginal effect,[23][4] and another review showing evidence for use of dietary phytosterols to attain a cholesterol-lowering effect.[24]
Coadministration of statins with phytosterol-enriched foods increases the cholesterol-lowering effect of phytosterols, again without any proof of clinical benefit and with anecdotal evidence of potentialadverse effects (though statins also have adverse effects such asmyopathy and digestive problems).[23] Statins work by reducing cholesterol synthesis via inhibition of the rate-limitingHMG-CoA reductaseenzyme. Phytosterols reduce cholesterol levels by competing with cholesterol absorption in the gut via one or several possible mechanisms,[25][26][27] an effect that complements statins. Phytosterols further reduce cholesterol levels by about 9% to 17% in statin users.[28] The type or dose of statin does not appear to affect the cholesterol-lowering efficacy of phytosterols.[29]
Because of their cholesterol reducing properties, some manufacturers are using sterols or stanols as a food additive.[3][30]
Phytosterols have a long history of safe use,[3] dating back to Cytellin, the pharmaceutical preparation of phytosterols marketed in the US from 1954 to 1982.[22] Phytosterol esters havegenerally recognized as safe (GRAS) status in the US.[31] Phytosterol-containingfunctional foods were subject to postlaunch monitoring after being introduced to the EU market in 2000, and no unpredicted side effects were reported.[32]
A potential safety concern regarding phytosterol consumption is in patients withphytosterolaemia, a rare genetic disorder which results in a 50- to 100-fold increase in blood plant sterol levels and is associated with rapid development of coronary atherosclerosis. Phytosterolaemia has been linked to mutations in the ABCG5/G8 proteins which pump plant sterols out of enterocytes and hepatocytes into the lumen and bile ducts, respectively. Plant sterol levels in the blood have been shown to be positively, negatively or not associated with CVD risk, depending on the study population investigated.[33][34][35][36][37][38][39][40]
The equivalent ability and safety of plant sterols and plantstanols to lower cholesterol continues to be a hotly debated topic. Plant sterols and stanols, when compared head-to-head in clinical trials, have been shown to equally reduce cholesterol levels.[41][42][43] A meta-analysis of 14 randomized, controlled trials comparing plant sterols to plant stanols directly at doses of 0.6 to 2.5 g/day showed no difference between the two forms on total cholesterol, LDL cholesterol, HDL cholesterol, or triglyceride levels.[44] Trials looking at high doses (> 4 g/day) of plant sterols or stanols are very limited, and none have yet to be completed comparing the same high dose of plant sterol to plant stanol.
The debate regarding sterol vs. stanol safety is centered on their differing intestinal absorption and resulting plasma concentrations. Phytostanols have a lower estimated intestinal absorption rate (0.02 - 0.3%) than phytosterols (0.4 - 5%) and consequently blood phytostanol concentration is generally lower than phytosterol concentration.[23]
Sterols are essential for alleukaryotes. In contrast to animal andfungal cells, which contain only one major sterol, plant cells synthesize an array of sterol mixtures in whichsitosterol andstigmasterol predominate.[45] Sitosterol regulates membrane fluidity and permeability in a similar manner to cholesterol in mammalian cell membranes.[46] Plant sterols can also modulate the activity of membrane-bound enzymes.[46] Phytosterols are also linked to plant adaptation to temperature and plant immunity against pathogens.[47]
^abMoreau, Robert A.; Nyström, Laura; Whitaker, Bruce D.; Winkler-Moser, Jill K.; Baer, David J.; Gebauer, Sarah K.; Hicks, Kevin B. (2018). "Phytosterols and their derivatives: Structural diversity, distribution, metabolism, analysis, and health-promoting uses".Progress in Lipid Research.70:35–61.doi:10.1016/j.plipres.2018.04.001.ISSN1873-2194.PMID29627611.
^Akhisa, T.; Kokke, W. (1991). "Naturally occurring sterols and related compounds from plants". In Patterson, G. W.; Nes, W. D. (eds.).Physiology and Biochemistry of Sterols. Champaign, IL: American Oil Chemists' Society. pp. 172–228.
^Andersson, S W; Skinner, J; Ellegård, L; Welch, A A; Bingham, S; Mulligan, A; Andersson, H; Khaw, K-T (2004). "Intake of dietary plant sterols is inversely related to serum cholesterol concentration in men and women in the EPIC Norfolk population: A cross-sectional study".European Journal of Clinical Nutrition.58 (10):1378–85.doi:10.1038/sj.ejcn.1601980.PMID15054420.S2CID19049641.
^Trautwein, Elke A.; Duchateau, Guus S. M. J. E.; Lin, Yuguang; Mel'nikov, Sergey M.; Molhuizen, Henry O.F.; Ntanios, Fady Y. (2003). "Proposed mechanisms of cholesterol-lowering action of plant sterols".European Journal of Lipid Science and Technology.105 (3–4):171–185.doi:10.1002/ejlt.200390033.
^De Smet, E; Mensink, RP; Plat, J (2012). "Effects of plant sterols and stanols on intestinal cholesterol metabolism: suggested mechanisms from past to present".Molecular Nutrition & Food Research.56 (7):1058–72.doi:10.1002/mnfr.201100722.PMID22623436.
^Scholle, JM; Baker, WL; Talati, R; Coleman, CI (2009). "The effect of adding plant sterols or stanols to statin therapy in hypercholesterolemic patients: Systematic review and meta-analysis".Journal of the American College of Nutrition.28 (5):517–24.doi:10.1080/07315724.2009.10719784.PMID20439548.S2CID41438503.
^Strandberg, Timo E.; Gylling, Helena; Tilvis, Reijo S.; Miettinen, Tatu A. (2010). "Serum plant and other noncholesterol sterols, cholesterol metabolism and 22-year mortality among middle-aged men".Atherosclerosis.210 (1):282–7.doi:10.1016/j.atherosclerosis.2009.11.007.PMID19962145.
^Fassbender, Klaus; Lütjohann, Dieter; Dik, Miranda G.; Bremmer, Marijke; König, Jochem; Walter, Silke; Liu, Yang; Letièmbre, Maryse; Von Bergmann, Klaus (2008). "Moderately elevated plant sterol levels are associated with reduced cardiovascular risk—The LASA study".Atherosclerosis.196 (1):283–8.doi:10.1016/j.atherosclerosis.2006.10.032.PMID17137582.
^Rajaratnam, Radhakrishnan A; Gylling, Helena; Miettinen, Tatu A (2000). "Independent association of serum squalene and noncholesterol sterols with coronary artery disease in postmenopausal women".Journal of the American College of Cardiology.35 (5):1185–91.doi:10.1016/S0735-1097(00)00527-1.PMID10758959.
^Assmann, Gerd; Cullen, Paul; Erbey, John; Ramey, Dena R.; Kannenberg, Frank; Schulte, Helmut (2006). "Plasma sitosterol elevations are associated with an increased incidence of coronary events in men: Results of a nested case-control analysis of the Prospective Cardiovascular Münster (PROCAM) study".Nutrition, Metabolism and Cardiovascular Diseases.16 (1):13–21.doi:10.1016/j.numecd.2005.04.001.PMID16399487.
^Sudhop, Thomas; Gottwald, Britta M.; Von Bergmann, Klaus (2002). "Serum plant sterols as a potential risk factor for coronary heart disease".Metabolism.51 (12):1519–21.doi:10.1053/meta.2002.36298.PMID12489060.
^O'Neill, F.H.; Brynes, A.; Mandeno, R.; Rendell, N.; Taylor, G.; Seed, M.; Thompson, G.R. (2004). "Comparison of the effects of dietary plant sterol and stanol esters on lipid metabolism".Nutrition, Metabolism and Cardiovascular Diseases.14 (3):133–42.doi:10.1016/S0939-4753(04)80033-4.PMID15330272.
^Talati, Ripple; Sobieraj, Diana M.; Makanji, Sagar S.; Phung, Olivia J.; Coleman, Craig I. (2010). "The Comparative Efficacy of Plant Sterols and Stanols on Serum Lipids: A Systematic Review and Meta-Analysis".Journal of the American Dietetic Association.110 (5):719–26.doi:10.1016/j.jada.2010.02.011.PMID20430133.
^Hartmann, Marie-Andrée (1998). "Plant sterols and the membrane environment".Trends in Plant Science.3 (5):170–175.doi:10.1016/S1360-1385(98)01233-3.
^abDe Smet, E; Mensink, R. P; Plat, J (2012). "Effects of plant sterols and stanols on intestinal cholesterol metabolism: Suggested mechanisms from past to present".Molecular Nutrition & Food Research.56 (7):1058–72.doi:10.1002/mnfr.201100722.PMID22623436.
