Microbial fuel cell (MFC) is a type of bioelectrochemicalfuel cell system[1] also known as micro fuel cell that generateselectric current by diverting electrons produced from the microbial oxidation of reduced compounds (also known as fuel orelectron donor) on the anode to oxidized compounds such asoxygen (also known as oxidizing agent orelectron acceptor) on the cathode through an externalelectrical circuit. MFCs produce electricity by using the electrons derived from biochemical reactionscatalyzed by bacteria.[2] MFCs can be grouped into two general categories: mediated and unmediated. The first MFCs, demonstrated in the early 20th century, used a mediator: a chemical that transfers electrons from the bacteria in the cell to the anode. Unmediated MFCs emerged in the 1970s; in this type of MFC the bacteria typically have electrochemically activeredoxproteins such ascytochromes on their outer membrane that can transfer electrons directly to the anode.[3][4] In the 21st century MFCs have started to find commercial use in wastewater treatment.[5]
The idea of using microbes to produceelectricity was conceived in the early twentieth century.Michael Cressé Potter initiated the subject in 1911.[6] Potter managed to generate electricity fromSaccharomyces cerevisiae, but the work received little coverage. In 1931,Barnett Cohen created microbialhalffuel cells that, when connected in series, were capable of producing over 35 volts with only a current of 2milliamps.[7]
A study by DelDuca et al. used hydrogen produced by thefermentation of glucose byClostridium butyricum as the reactant at the anode of a hydrogen and air fuel cell. Though the cell functioned, it was unreliable owing to the unstable nature ofhydrogen production by the micro-organisms.[8] This issue was resolved by Suzuki et al. in 1976,[9] who produced a successful MFC design a year later.[10]
In the late 1970s, little was understood about how microbial fuel cells functioned. The concept was studied by Robin M. Allen and later by H. Peter Bennetto. People saw the fuel cell as a possible method for the generation of electricity for developing countries. Bennetto's work, starting in the early 1980s, helped build an understanding of how fuel cells operate and he was seen by some[who?] as the topic's foremost authority.
In May 2007, theUniversity of Queensland, Australia completed a prototype MFC as a cooperative effort withFoster's Brewing. The prototype, a 10 L design, convertedbrewery wastewater into carbon dioxide, clean water and electricity. The group had plans to create a pilot-scale model for an upcoming international bio-energy conference.[11]
A microbial fuel cell (MFC) is a device thatconvertschemical energy toelectrical energy by the action ofmicroorganisms.[12] These electrochemical cells are constructed using either a bioanode and/or a biocathode. Most MFCs contain a membrane to separate the compartments of the anode (where oxidation takes place) and the cathode (where reduction takes place). The electrons produced during oxidation are transferred directly to an electrode or to aredox mediator species. The electron flux is moved to the cathode. The charge balance of the system is maintained by ionic movement inside the cell, usually across an ionic membrane. Most MFCs use an organicelectron donor that is oxidized to produce CO2, protons, and electrons. Other electron donors have been reported, such as sulfur compounds or hydrogen.[13] The cathode reaction uses a variety of electron acceptors, most oftenoxygen (O2). Other electron acceptors studied include metal recovery by reduction,[14] water to hydrogen,[15] nitrate reduction,[16][17] and sulfate reduction.
MFCs are attractive for power generation applications that require only low power, but where replacing batteries may be impractical, such aswireless sensor networks.[18][19][20]Wireless sensors powered by microbial fuel cells can then for example be used forremote monitoring (conservation).[21]
Virtually any organic material could be used to feed the fuel cell, including coupling cells towastewater treatment plants. Chemical process wastewater[22][23] and synthetic wastewater[24][25] have been used to produce bioelectricity in dual- and single-chamber mediator less MFCs (uncoated graphite electrodes).
Higher power production was observed with abiofilm-covered graphiteanode.[26][27] Fuel cell emissions are well under regulatory limits.[28] MFCs convert energy more efficiently than standardinternal combustion engines, which are limited by theCarnot efficiency. In theory, an MFC is capable of energy efficiency far beyond 50%.[29] Rozendal produced hydrogen with 8 times less energy input than conventional hydrogen production technologies.
Moreover, MFCs can also work at a smaller scale. Electrodes in some cases need only be 7 μm thick by 2 cm long,[30] such that an MFC can replace a battery. It provides a renewable form of energy and does not need to be recharged.
MFCs operate well in mild conditions, 20 °C to 40 °C and atpH of around 7[31] but lack the stability required for long-term medical applications such as inpacemakers.
Power stations can be based on aquatic plants such as algae. If sited adjacent to an existing power system, the MFC system can share its electricity lines.[32]
Soil-based microbial fuel cells serve as educational tools, as they encompass multiple scientific disciplines (microbiology, geochemistry, electrical engineering, etc.) and can be made using commonly available materials, such as soils and items from the refrigerator. Kits for home science projects and classrooms are available.[33] One example of microbial fuel cells being used in the classroom is in the IBET (Integrated Biology, English, and Technology) curriculum forThomas Jefferson High School for Science and Technology. Several educational videos and articles are also available on theInternational Society for Microbial Electrochemistry and Technology (ISMET Society)"[34]".
The current generated from a microbial fuel cell is directly proportional to the organic-matter content of wastewater used as the fuel. MFCs can measure the solute concentration of wastewater (i.e., as abiosensor).[35]
Wastewater is commonly assessed for itsbiochemical oxygen demand (BOD) values.[clarification needed] BOD values are determined by incubating samples for 5 days with proper source of microbes, usually activated sludge collected from wastewater plants.
An MFC-type BOD sensor can provide real-time BOD values. Oxygen and nitrate are interfering preferred electron acceptors over the anode, reducing current generation from an MFC. Therefore, MFC BOD sensors underestimate BOD values in the presence of these electron acceptors. This can be avoided by inhibiting aerobic and nitrate respiration in the MFC using terminal oxidase inhibitors such ascyanide andazide.[36] Such BOD sensors are commercially available.
TheUnited States Navy is considering microbial fuel cells for environmental sensors. The use of microbial fuel cells to power environmental sensors could provide power for longer periods and enable the collection and retrieval of undersea data without a wired infrastructure. The energy created by these fuel cells is enough to sustain the sensors after an initial startup time.[37] Due to undersea conditions (high salt concentrations, fluctuating temperatures and limited nutrient supply), the Navy may deploy MFCs with a mixture of salt-tolerant microorganisms that would allow for a more complete utilization of available nutrients.Shewanella oneidensis is their primary candidate, but other heat- and cold-tolerantShewanella spp may also be included.[38]
A first self-powered and autonomous BOD/COD biosensor has been developed and enables detection of organic contaminants in freshwater. The sensor relies only on power produced by MFCs and operates continuously without maintenance. It turns on the alarm to inform about contamination level: the increased frequency of the signal warns about a higher contamination level, while a low frequency informs about a low contamination level.[39]
In 2010, A. ter Heijne et al.[40] constructed a device capable of producing electricity and reducing Cu2+ ions to copper metal.
Microbial electrolysis cells have been demonstrated to produce hydrogen.[41]
MFCs are used in water treatment to harvest energy utilizinganaerobic digestion. The process can also reduce pathogens. However, it requires temperatures upwards of 30 degrees C and requires an extra step in order to convertbiogas to electricity. Spiral spacers may be used to increase electricity generation by creating a helical flow in the MFC. Scaling MFCs is a challenge because of the power output challenges of a larger surface area.[42]
Most microbial cells are electrochemically inactive. Electron transfer from microbial cells to theelectrode is facilitated by mediators such asthionine,pyocyanin,[43]methyl viologen,methyl blue,humic acid, andneutral red.[44][45] Most available mediators are expensive and toxic.
Mediator-free microbial fuel cells use electrochemically active bacteria such asShewanella putrefaciens[46] andAeromonas hydrophila[47] to transfer electrons directly from the bacterial respiratory enzyme to the electrode. Some bacteria are able to transfer their electron production via thepili on their external membrane. Mediator-free MFCs are less well characterized, such as thestrain of bacteria used in the system, type ofion-exchange membrane and system conditions (temperature, pH, etc.)
Mediator-free microbial fuel cells can run onwastewater and derive energy directly from certain plants and O2. This configuration is known as a plant microbial fuel cell. Possible plants includereed sweetgrass,cordgrass, rice, tomatoes,lupines andalgae.[48][49][50] Given that the power is obtained using living plants (in situ-energy production), this variant can provide ecological advantages.
One variation of the mediator-less MFC is the microbial electrolysis cell (MEC). While MFCs produce electric current by the bacterial decomposition of organic compounds in water, MECs partially reverse the process to generate hydrogen or methane by applying a voltage to bacteria. This supplements the voltage generated by the microbial decomposition of organics, leading to theelectrolysis of water or methane production.[51][52] A complete reversal of the MFC principle is found inmicrobial electrosynthesis, in whichcarbon dioxide is reduced by bacteria using an external electric current to form multi-carbon organic compounds.[53]
Soil-based microbial fuel cells adhere to the basic MFC principles, whereby soil acts as the nutrient-rich anodic media, theinoculum and theproton exchange membrane (PEM). Theanode is placed at a particular depth within the soil, while thecathode rests on top the soil and is exposed to air.
Soils naturallyteem with diverse microbes, includingelectrogenic bacteria needed for MFCs, and are full of complex sugars and other nutrients that have accumulated from plant and animal material decay. Moreover, theaerobic (oxygen consuming) microbes present in the soil act as an oxygen filter, much like the expensive PEM materials used in laboratory MFC systems, which cause theredox potential of the soil to decrease with greater depth. Soil-based MFCs are becoming popular educational tools for science classrooms.[33]
Sediment microbial fuel cells (SMFCs) have been applied forwastewater treatment. Simple SMFCs can generate energy while decontaminatingwastewater. Most such SMFCs contain plants to mimic constructed wetlands. By 2015 SMFC tests had reached more than 150 L.[54]
In 2015 researchers announced an SMFC application that extracts energy and charges abattery. Salts dissociate into positively and negatively charged ions in water and move and adhere to the respective negative and positive electrodes, charging the battery and making it possible to remove the salt effectingmicrobial capacitivedesalination. The microbes produce more energy than is required for the desalination process.[55] In 2020, a European research project achieved the treatment of seawater into fresh water for human consumption with an energy consumption around 0.5 kWh/m3, which represents an 85% reduction in current energy consumption respect state of the art desalination technologies. Furthermore, the biological process from which the energy is obtained simultaneously purifies residual water for its discharge in the environment or reuse in agricultural/industrial uses. This has been achieved in the desalination innovation center that Aqualia has opened in Denia, Spain early 2020.[56]
Phototrophic biofilm MFCs (ner) use a phototrophicbiofilm anode containing photosynthetic microorganism such aschlorophyta andcandyanophyta. They carry out photosynthesis and thus produce organic metabolites and donate electrons.[57]
One study found that PBMFCs display apower density sufficient for practical applications.[58]
The sub-category of phototrophic MFCs that use purely oxygenic photosynthetic material at the anode are sometimes calledbiological photovoltaic systems.[59]
TheUnited States Naval Research Laboratory developed nanoporous membrane microbial fuel cells that use a non-PEM to generate passive diffusion within the cell.[60] The membrane is a nonporous polymer filter (nylon,cellulose, orpolycarbonate). It offers comparable power densities toNafion (a well-known PEM) with greater durability. Porous membranes allow passive diffusion thereby reducing the necessary power supplied to the MFC in order to keep the PEM active and increasing the total energy output.[61]
MFCs that do not use a membrane can deploy anaerobic bacteria in aerobic environments. However, membrane-less MFCs experience cathode contamination by the indigenous bacteria and the power-supplying microbe. The novel passive diffusion of nanoporous membranes can achieve the benefits of a membrane-less MFC without worry of cathode contamination.Nanoporous membranes are also 11 times cheaper than Nafion (Nafion-117, $0.22/cm2 vs. polycarbonate, <$0.02/cm2).[62]
PEM membranes can be replaced with ceramic materials.Ceramic membrane costs can be as low as $5.66/m2. The macroporous structure of ceramic membranes allows for good transport of ionic species.[63]
The materials that have been successfully employed in ceramic MFCs areearthenware,alumina,mullite,pyrophyllite, andterracotta.[63][64][65]
When microorganisms consume a substance such assugar in aerobic conditions, they producecarbon dioxide andwater. However, whenoxygen is not present, they may produce carbon dioxide,hydrons (hydrogen ions), andelectrons, as described below forsucrose:[66]
| C12H22O11 + 13H2O → 12CO2 + 48H+ + 48e− | Eqt. 1 |
Microbial fuel cells useinorganic mediators to tap into theelectron transport chain of cells and channel electrons produced. The mediator crosses the outer celllipid membranes andbacterial outer membrane; then, it begins to liberate electrons from the electron transport chain that normally would be taken up by oxygen or other intermediates.
The now-reduced mediator exits the cell laden with electrons that it transfers to an electrode; this electrode becomes the anode. The release of the electrons recycles the mediator to its original oxidized state, ready to repeat the process.This can happen only under anaerobic conditions; if oxygen is present, it will collect the electrons, as it has morefree energy to release.
Certain bacteria can circumvent the use of inorganic mediators by making use of special electron transport pathways known collectively asextracellular electron transfer (EET). EET pathways allow the microbe to directly reduce compounds outside of the cell, and can be used to enable direct electrochemical communication with the anode.[67]
In MFC operation, the anode is the terminal electron acceptor recognized by bacteria in the anodic chamber. Therefore, the microbial activity is strongly dependent on the anode's redox potential. AMichaelis–Menten curve was obtained between the anodic potential and the power output of anacetate-driven MFC. A critical anodic potential seems to provide maximum power output.[68]
Potential mediators include natural red, methylene blue, thionine, and resorufin.[69]
Organisms capable of producing an electric current are termedexoelectrogens. In order to turn this current into usable electricity, exoelectrogens have to be accommodated in a fuel cell.
The mediator and a micro-organism such as yeast, are mixed together in a solution to which is added a substrate such asglucose. This mixture is placed in a sealed chamber to prevent oxygen from entering, thus forcing the micro-organism to undertakeanaerobic respiration. An electrode is placed in the solution to act as the anode.
In the second chamber of the MFC is another solution and the positively charged cathode. It is the equivalent of the oxygen sink at the end of the electron transport chain, external to the biological cell. The solution is anoxidizing agent that picks up the electrons at the cathode. As with the electron chain in the yeast cell, this could be a variety of molecules such as oxygen, although a more convenient option is a solid oxidizing agent, which requires less volume.
Connecting the two electrodes is a wire (or other electrically conductive path). Completing the circuit and connecting the two chambers is a salt bridge or ion-exchange membrane. This last feature allows the protons produced, as described inEqt. 1, to pass from the anode chamber to the cathode chamber.
The reduced mediator carries electrons from the cell to the electrode. Here the mediator is oxidized as it deposits the electrons. These then flow across the wire to the second electrode, which acts as an electron sink. From here they pass to an oxidizing material. Also the hydrogen ions/protons are moved from the anode to the cathode via a proton exchange membrane such asNafion. They will move across to the lower concentration gradient and be combined with the oxygen but to do this they need an electron. This generates current and the hydrogen is used sustaining the concentration gradient.
Algal biomass has been observed to give high energy when used as the substrate in microbial fuel cell.[70]
Microbial fuel cells (MFCs) have emerged as promising tools for environmental remediation due to their unique ability to utilize the metabolic activities of microorganisms for both electricity generation and pollutant degradation.[71] MFCs find applications across diverse contexts in environmental remediation. One primary application is in bioremediation, where the electroactive microorganisms on the MFC anode actively participate in the breakdown of organic pollutants, providing a sustainable and efficient method for pollutant removal. Moreover, MFCs play a significant role in wastewater treatment by simultaneously generating electricity and enhancing water quality through the microbial degradation of contaminants. These fuel cells can be deployed in situ, allowing for continuous and autonomous remediation in contaminated sites. Furthermore, their versatility extends to sediment microbial fuel cells (SMFCs), which are capable of removing heavy metals and nutrients from sediments.[72] By integrating MFCs with sensors, they enable remote environmental monitoring in challenging locations. The applications of microbial fuel cells in environmental remediation highlight their potential to convert pollutants into a renewable energy source while actively contributing to the restoration and preservation of ecosystems.
Microbial fuel cells (MFCs) offer significant potential as sustainable and innovative technologies, but they are not without their challenges. One major obstacle lies in the optimization of MFC performance, which remains a complex task due to various factors including microbial diversity, electrode materials, and reactor design.[73] The development of cost-effective and long-lasting electrode materials presents another hurdle, as it directly affects the economic viability of MFCs on a larger scale. Furthermore, the scaling up of MFCs for practical applications poses engineering and logistical challenges. Nonetheless, ongoing research in microbial fuel cell technology continues to address these obstacles. Scientists are actively exploring new electrode materials, enhancing microbial communities to improve efficiency, and optimizing reactor configurations. Moreover, advancements in synthetic biology and genetic engineering have opened up possibilities for designing custom microbes with enhanced electron transfer capabilities, pushing the boundaries of MFC performance.[74] Collaborative efforts between multidisciplinary fields are also contributing to a deeper understanding of MFC mechanisms and expanding their potential applications in areas such as wastewater treatment, environmental remediation, and sustainable energy production.
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