Hepatocystis is a genus of parasites transmitted by midges of the genusCulicoides. Hosts include Old Worldprimates,bats,hippopotamus andsquirrels. This genus is not found in the New World. The genus was erected by Levaditi and Schoen, 1932, asHepatocystes.
The first observation of malaria in bats dates to the end of the 19th century when Dionisi described gametocytes in the blood of a bat in 1898. The type species of this genus (Hepatocystis kochi) was described by Laveran in 1899 from the blood of a primate and he named itPlasmodium kochi.
The protozoa are transmitted by the bite of the insect vector. The sporozoites migrate to the liver where they typically formmerocysts within the liver parenchyma. These may also develop in the spleen and lungs. Macroscopically these may appear as white to gray nodular foci within the tissue. These lesions within these organs are characterized by well-circumscribed focal fibrosis, accumulation ofeosinophils andhistiocytes,hemorrhage orhemosiderosis with the loss of normal architecture.
The cystlike exoerythrocyticschizonts are found within the merocysts. These are found around the edges of the merocytes in huge numbers. The central core of the cyst consists of a non-cellular granular material.
Released merozoites either invade other hepatocytes orerythrocytes. Within the erythrocytes the merozoites first become ring forms, thentrophozoites and finallygametocytes. The gametocytes are huge and fill the entire erythrocyte. Like those ofPlasmodium and unlike those ofHepatozoon their nuclei areFeulgen negative.Haemozoin may be found within the erythrocytes.
Infected erythrocytes are then taken up when the insect vector feeds on the host. Within the vector the sporogonic cycle lasts between 5–6 days. Theoocysts develop in thehaemocoele chiefly in the head near the brain and eye.
The maturemerocysts are visible to the naked eye on the hepatic surface. These appear as raised, grayish-white to translucent foci with a central accumulation of fluid. Multiple, depressed areas of fibrosis with calcification representing healed lesions may also be found.
Microscopically the liver cysts have an irregular central space filled with faintly eosinophilic, acellular, flocculent material. Surrounding this is a peripheral rim of myriad, round, lightly basophilicmerozoites which measure ~1.0–2.0 mm in diameter. The cysts themselves are delineated by a thin, convoluted, eosinophilic, hyaline capsule. A significant inflammatory response to the merocysts may or may not be present. If a reaction is present, it is typically granulomatous with an admixture ofeosinophils andlymphocytes.
The first appearance in the blood is normally two months after infection. The only stage seen in the blood are gametocytes. These double in number for the first three days and mature. Having reached maturity, they gradually decline in number and normally disappear after a month. Secondary episodes may occur at variable intervals.
The early gametocytes consist of minute dense spots of chromatin with a tiny loop of cytoplasm. As the parasite grows, the chromatin tends to spread in a semicircle or into multiple dots. There is no stippling of the erythrocyte.
Mature gametocytes are larger than a normal erythrocyte stain poorly compared to other protozoa. In both male and female gametocytes, the nucleus consists of two portions.
Themacrogametocyte is a steel blue color measuring 9.5 micrometres in diameter. The nucleus has a pale pink area with dense chromatin in the middle and is much smaller than that of the microgametocyte.
Themicrogametocyte stains less densely, is biscuit-colored and measures 9.0 micrometres in diameter. The nucleus is a large, oval, pale pink area occupying one-third to one-half of the parasite.
In endemic areas, the incidence can range from 24 to 75% in nonhuman primates. Although infection withHepatocystis does not normally cause fever it may cause anaemia in the host.
Some versions of theDuffy antigen have been associated with protection fromHepatocytis infection in yellow baboons (Papio cynocephalus).[1]
Primates and bats are the most commonly identified host of these parasites.H. bouillezi,H. cercopitheci,H. kochi andH. simiae infectAfrican monkeys.H. semnopitheci andH. taiwanensis infectAsian monkeys. These species may lie in different phylogenetic clades but this is not yet known.
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^abcdeManwell RD, Kuntz RE (1966). "Hepatocystis in Formosan mammals with a description of a new species".J. Euk Microbiol.13 (4):670–2.doi:10.1111/j.1550-7408.1966.tb01979.x.
^abOlival KJ, Stiner EO, Perkins SL (2007). "Detection of Hepatocystis sp. in southeast Asian flying foxes (Pteropodidae) using microscopic and molecular methods".J. Parasitol.93 (6):1538–40.doi:10.1645/GE-1208.1.PMID18314711.
^Masbar S, Palmieri JR, Marwoto HA, Purnomo, Darwis F (1981). "Blood parasites of wild and domestic animals from South Kalimantan (Borneo), Indonesia".Southeast Asian J. Trop. Med. Public Health.12 (1):42–6.PMID6789456.
^Shiroishi T, Davis J, Warren M (1968). "Hepatocystis in the white-cheeked gibbon, Hylobates concolor".J. Parasitol.54 (1): 168.doi:10.2307/3276896.JSTOR3276896.PMID4966669.
^Zeiss CJ, Shomer N (2001). "Hepatocystosis in a baboon (Papio anubis)".Contemp Top Lab Anim Sci.40 (1):41–2.PMID11300677.
^Stafford EE, Galdikas-Brindamour B, Beaudoin, et al. (1978). "Hepatocystis in the orangutan,Pongo pygmaeus".Trans. R. Soc. Trop. Med. Hyg.72 (1):106–7.doi:10.1016/0035-9203(78)90314-0.PMID416523.
