Buchner was appointed assistant lecturer in the organic laboratory ofAdolf von Baeyer in 1889 at the University of Munich. In 1891, he was promoted to lecturer at the same university.[1]
In the autumn of 1893, Buchner moved toUniversity of Kiel and appointed professor in 1895. In the next year he was appointed Professor Extraordinary for Analytical and Pharmaceutical Chemistry in the chemical laboratory of H. von Pechmann at theUniversity of Tübingen.[1]
In October, 1898, he was appointed to the Chair of General Chemistry in theAgricultural University of Berlin, fully training his assistants by himself, and received his habilitation in 1900.[1][4]
Buchner received theNobel Prize in Chemistryin 1907.[1] The experiment for which Buchner won the Nobel Prize consisted of producing acell-free extract ofyeast cells and showing that this "press juice" could ferment sugar. This dealt yet another blow tovitalism by showing that the presence of living yeast cells was not needed for fermentation. The cell-free extract was produced by combining dry yeast cells,quartz andkieselguhr and then pulverizing the yeast cells with a pestle and mortar. This mixture would then become moist as the yeast cells' contents would come out of the cells. Once this step was done, the moist mixture would be put through a press and the resulting "press juice" hadglucose,fructose,ormaltose added andcarbon dioxide was seen to evolve, sometimes for days. Microscopic investigation revealed no living yeast cells in the extract. Buchner hypothesized that yeast cells secrete proteins into their environment in order to ferment sugars, but it was later found that fermentation occurs inside the yeast cells.Maria Manasseina claimed to have discovered free-cell fermentation a generation earlier than Buchner,[6] but Buchner and Rapp considered that she was subjectively convinced of the existence of an enzyme of fermentation, and that her experimental evidence was unconvincing.[7]
Buchner married Lotte Stahl in 1900. At the outbreak of theFirst World War, he volunteered in theImperial German Army and rose to the rank ofMajor, commanding a munition-transport unit on theWestern and thenEastern Front. In March 1916, he returned the University of Würzburg. In April 1917, he volunteered again. On 11 August 1917, while stationed atFocșani, Romania, he was hit by a shell fragment in the left thigh and died in a field hospital two days later.[8] He died in theBattle of Mărășești and is buried in the cemetery of German soldiers in Focșani.[8]
Robert Kohler (1971). "The background to Eduard Buchner's discovery of cell-free fermentation".Journal of the History of Biology.4 (1):35–61.doi:10.1007/BF00356976.PMID11609437.S2CID46573308.
Robert Kohler (1972). "The reception of Eduard Buchner's discovery of cell-free fermentation".Journal of the History of Biology.5 (2):327–353.doi:10.1007/BF00346663.PMID11610124.S2CID34944527.
^Buchner E, Rapp, R (1898). "Alkoholische Gährung ohne Hefezellen".Berichte der Deutschen Chemischen Gesellschaft.30:209–217.{{cite journal}}: CS1 maint: multiple names: authors list (link)
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