Protein-coding gene in the species Homo sapiens
Eukaryotic translation initiation factor 5B is aprotein that in humans is encoded by theEIF5B gene .[ 5] [ 6] [ 7] bacterial initiation factor 2 , sharing a core structure while their involvement in translation initiation differs.[ 8]
eIF5B plays an important role in later stages of the translation initiation mechanism. After the start codon has been engaged by the scanning initiation complex, eukaryotic initiation factorseIF1 ,eIF2 , andeIF5 leave theribosome . Subsequently, eIF5B-GTP is recruited to the ribosome.eIF1A induces rotation of a domain of eIF5B-GTP, which in turn positions the initiatortRNA such that joining of the large ribosomal subunit is possible.[ 9] [ 10] [ 11]
^a b c GRCh38: Ensembl release 89: ENSG00000158417 –Ensembl , May 2017^a b c GRCm38: Ensembl release 89: ENSMUSG00000026083 –Ensembl , May 2017^ "Human PubMed Reference:" .National Center for Biotechnology Information, U.S. National Library of Medicine .^ "Mouse PubMed Reference:" .National Center for Biotechnology Information, U.S. National Library of Medicine .^ Lee JH, Choi SK, Roll-Mecak A, Burley SK, Dever TE (May 1999)."Universal conservation in translation initiation revealed by human and archaeal homologs of bacterial translation initiation factor IF2" .Proc Natl Acad Sci U S A .96 (8):4342– 7.Bibcode :1999PNAS...96.4342L .doi :10.1073/pnas.96.8.4342 PMC 16334 PMID 10200264 . ^ Wilson SA, Sieiro-Vazquez C, Edwards NJ, Iourin O, Byles ED, Kotsopoulou E, Adamson CS, Kingsman SM, Kingsman AJ, Martin-Rendon E (Oct 1999)."Cloning and characterization of hIF2, a human homologue of bacterial translation initiation factor 2, and its interaction with HIV-1 matrix" .Biochem J . 342. ( Pt 1) (Pt 1):97– 103.doi :10.1042/bj3420097 .PMC 1220441 PMID 10432305 . ^ "Entrez Gene: EIF5B eukaryotic translation initiation factor 5B" .^ Unbehaun, Anett; Marintchev, Assen; Lomakin, Ivan B.; Didenko, Tatyana; Wagner, Gerhard; Hellen, Christopher U T.; Pestova, Tatyana V. (2007)."Position of eukaryotic initiation factor eIF5B on the 80S ribosome mapped by directed hydroxyl radical probing" .The EMBO Journal .26 (13):3109– 3123.doi :10.1038/sj.emboj.7601751 .PMC 1914099 PMID 17568775 . ^ Lapointe, Christopher P.; Grosely, Rosslyn; Sokabe, Masaaki; Alvarado, Carlos; Wang, Jinfan; Montabana, Elizabeth; Villa, Nancy; Shin, Byung-Sik; Dever, Thomas E.; Fraser, Christopher S.; Fernández, Israel S.; Puglisi, Joseph D. (2022)."EIF5B and eIF1A reorient initiator tRNA to allow ribosomal subunit joining" .Nature .607 (7917):185– 190.Bibcode :2022Natur.607..185L .doi :10.1038/s41586-022-04858-z .PMC 9728550 PMID 35732735 . ^ Fringer, Jeanne M.; Acker, Michael G.; Fekete, Christie A.; Lorsch, Jon R.; Dever, Thomas E. (2007)."Coupled Release of Eukaryotic Translation Initiation Factors 5B and 1A from 80S Ribosomes following Subunit Joining" .Molecular and Cellular Biology .27 (6):2384– 2397.doi :10.1128/MCB.02254-06 .PMC 1820483 PMID 17242201 . ^ Wang, Jinfan; Wang, Jing; Shin, Byung-Sik; Kim, Joo-Ran; Dever, Thomas E.; Puglisi, Joseph D.; Fernández, Israel S. (2020)."Structural basis for the transition from translation initiation to elongation by an 80S-eIF5B complex" .Nature Communications .11 (1) 5003.Bibcode :2020NatCo..11.5003W .doi :10.1038/s41467-020-18829-3 .PMC 7538418 PMID 33024099 . A comprehensive review on eukaryotic translation initiation :Brito Querido, Jailson; Díaz-López, Irene; Ramakrishnan, V. (2024)."The molecular basis of translation initiation and its regulation in eukaryotes" Nature Reviews Molecular Cell Biology .25 (3):168– 186.doi :10.1038/s41580-023-00624-9 .PMID 38052923 . The structure of an 80S-eIF5B complex :Wang, Jinfan; Wang, Jing; Shin, Byung-Sik; Kim, Joo-Ran; Dever, Thomas E.; Puglisi, Joseph D.; Fernández, Israel S. (2020)."Structural basis for the transition from translation initiation to elongation by an 80S-eIF5B complex" .Nature Communications .11 5003.Bibcode :2020NatCo..11.5003W .doi :10.1038/s41467-020-18829-3 .PMC 7538418 PMID 33024099 . Marintchev A, Kolupaeva VG, Pestova TV, Wagner G (2003)."Mapping the binding interface between human eukaryotic initiation factors 1A and 5B: a new interaction between old partners" .Proc. Natl. Acad. Sci. U.S.A .100 (4):1535– 40.Bibcode :2003PNAS..100.1535M .doi :10.1073/pnas.0437845100 PMC 149867 PMID 12569173 . Olsen DS, Savner EM, Mathew A, et al. (2003)."Domains of eIF1A that mediate binding to eIF2, eIF3 and eIF5B and promote ternary complex recruitment in vivo" .EMBO J .22 (2):193– 204.doi :10.1093/emboj/cdg030 .PMC 140105 PMID 12514125 .
