DAOY is a widely used humanmedulloblastomacell line. It hasepithelialmorphology and was obtained from a 4-year-old Caucasian boy in 1985.[1] It can be obtained from theAmerican Type Culture Collection (ATCC) where it is known by the catalog number HTB-186.[2] Its proteome and phenotype have been investigated in many publications.[3][4][5]
DAOY is most commonly grown inEagle's minimal essential medium with 10%FBS and antibiotics. It is incubated at 37 degrees Celsius in a 5% carbon dioxide atmosphere.
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