| Crescentin | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| Symbol | Crescentin | ||||||||
| Pfam | PF19220 | ||||||||
| InterPro | IPR043652 | ||||||||
| |||||||||
| Intermediate filament-like cell shape determinant CreS | |||||||
|---|---|---|---|---|---|---|---|
| Identifiers | |||||||
| Organism | Caulobacter vibrioides | ||||||
| Symbol | CreS | ||||||
| Alt. symbols | ParA | ||||||
| UniProt | Q6IET3 | ||||||
| |||||||
Crescentin is aprotein which is abacterial relative of theintermediate filaments found ineukaryotic cells. Just astubulins andactins, the other majorcytoskeletal proteins, haveprokaryotic homologs in, respectively, theFtsZ andMreB proteins, intermediate filaments are linked to the crescentin protein. Some of itshomologs are erroneously labelledChromosome segregation protein ParA. Thisprotein family is found inCaulobacter andMethylobacterium.
Crescentin was discovered in 2009 byChristine Jacobs-Wagner inCaulobacter crescentus (nowvibrioides), an aquatic bacterium which uses itscrescent-shaped cells for enhanced motility.[1] The crescentin protein is located on theconcave face of these cells and appears to be necessary for their shape, asmutants lacking the protein form rod-shaped cells.[2] To influence the shape of theCaulobacter cells, thehelices of crescentin filaments associate with thecytoplasmic side of thecell membrane on one lateral side of the cell. This induces a curved cell shape in younger cells, which are shorter than the helical pitch of crescentin, but induces a spiral shape in older, longer cells.[3]
Like eukaryotic intermediate filaments, crescentin organizes into filaments and is present in a helical structure in the cell. Crescentin is necessary for both shapes of theCaulobacter prokaryote (vibroid/crescent-shape and helical shape, which it may adopt after a long stationary phase). The crescentin protein has 430 residues; its sequence mostly consists of a pattern of 7 repeated residues which form a coiled-coil structure. TheDNA sequence of the protein has sections very similar to the eukaryotickeratin andlamin proteins, mostly involving the coiled-coil structure. Ausmees et al. (2003) proved that, like animal intermediate filament proteins, crescentin has a central rod made up of four coiled-coil segments.[4] Both intermediate filament and crescentin proteins have a primary sequence including four α-helical segments along with non-α-helical linker domains. An important difference between crescentin and animal intermediate filament proteins is that crescentin lacks certain consensus sequence elements at the ends of the rod domain which are conserved in animal lamin and keratin proteins.[5]
The protein has been divided into a few subdomains organized similarly to eukaryotic IF proteins.[6] Not every researcher is convinced that it is a homolog of intermediate filaments, suggesting instead that the similarity might have arisen via convergent evolution.[7] (Indeed, the Cryo-EM structure of CreS does not display the proposed eukaryotic-like interruption in the rod; see next paragraph.)
A number ofCryo-EM structures of crescentin were published in late 2023. The researchers used ananobody that tags onto one specific part of the filament, so that it's easier to tell where each unit of the filament begins and ends. Two chains of the crescentin molecule pair together into a dimeric coil. Two coils come together side-by-side into a strand. Each strand is paired at its head and tail by another strand, so that it continues like a chain. Two chains of strands pair together side-by-side into a filament. Like eukaryotic intermediate filamenets, the CreS filament is octameric and lacks overall polarity. However, CreS does not show a linker domain in the middle but instead presents as a continuous rod.[8]
Eukaryotic intermediate filament proteins assemble into filaments of 8–15 nm within the cell without the need for energy input, that is, no need forATP orGTP. Ausmees et al. continued their crescentin research by testing whether the protein could assemble into filaments in this mannerin vitro. They found that crescentin proteins were indeed able to form filaments about 10 nm wide, and that some of these filaments organized laterally into bundles, just as eukaryotic intermediate filaments do.[4] The similarity of crescentin protein to intermediate filament proteins suggests anevolutionary linkage between these two cytoskeletal proteins.
Like eukaryotic intermediate filaments, the filament built from crescentin is elastic. Individual proteins dissociate slowly, making the structure somewhat stiff and slow to remodel. Strain does not induce hardening of the structure, unlike eukaryotic IFs that do.[9]