Bismuth sulfite agar is a type ofagar media used to isolateSalmonella species. It usesglucose as a primary source ofcarbon. Bismuth andbrilliant green (dye) both inhibitgram-positive growth.Bismuth sulfite agar tests the ability to useferrous sulfate and convert it tohydrogen sulfide.

Bismuth sulfite agar typically contains (w/v):^[1]

1.6%bismuth sulfite Bi₂(SO₃)₃

1.0% pancreaticdigest ofcasein

1.0% pancreatic digest of animaltissue

1.0%beef extract

1.0%glucose

0.8% dibasicsodium phosphate

0.06%ferrous sulfate • 7 water

pH adjusted to 7.7 at 25 °C

This medium is boiled for sterility, notautoclaved.

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