| ANTH domain | |||||||||
|---|---|---|---|---|---|---|---|---|---|
 Clathrin assembly lymphoid myeloid leukemia (CALM) protein | |||||||||
| Identifiers | |||||||||
| Symbol | ANTH | ||||||||
| Pfam | PF07651 | ||||||||
| InterPro | IPR011417 | ||||||||
| OPM superfamily | 38 | ||||||||
| OPM protein | 1hfa | ||||||||
| CDD | cd03564 | ||||||||
| |||||||||
TheANTH domain is a membrane binding domain that shows weak specificity forPtdIns(4,5)P2. It was found inAP180 (homologous to CALM[1]) endocytotic accessory protein that has been implicated in the formation of clathrin-coated pits. The domain is involved in phosphatidylinositol 4,5-bisphosphate binding and is a universal adaptor for nucleation of clathrin coats.[2][3]
Its structure is a solenoid of 9 helices. The PtdIns(4,5)P2 binding residues are spread over several helices at the tip of the structure. The PtdIns(4,5)P2 binding sequence is Kx9Kx(K/R)(H/Y).
An ANTH domain is also found inHIP1 andHIP1R, and the PtdIns(4,5)P2 binding sequence is conserved.